Joost Wijnakker
Research PhD at Hubrecht Institute, Developmental Biology and Stem Cell Research- Claim this Profile
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Dutch Native or bilingual proficiency
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English Full professional proficiency
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French Elementary proficiency
Topline Score
Bio
Credentials
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Bachelor of science
TU Delft -
International Baccalaureate
Maaslandcollege
Experience
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Hubrecht Institute, Developmental Biology and Stem Cell Research
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Netherlands
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Research Services
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200 - 300 Employee
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Research PhD
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Jun 2021 - Present
Lab of Prof. Dr. J.C. Hans Clevers Research area: Material driven regeneration Hubrecht institute, MDR Research Project: Producing new biomaterials for epithelial organoid formation Lab of Prof. Dr. J.C. Hans Clevers Research area: Material driven regeneration Hubrecht institute, MDR Research Project: Producing new biomaterials for epithelial organoid formation
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Hubrecht Institute, Developmental Biology and Stem Cell Research
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Netherlands
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Research Services
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200 - 300 Employee
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Research Internship - Minor project
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Sep 2020 - Feb 2021
Lab of Dr. Katharina Sonnen, Junior Group Leader Research area: Signal dynamics in tissue development Hubrecht institute, Utrecht Project: Notch signaling dynamics in the intestinal epithelium KEY ACHIEVEMENTS ----------------------------------------------------------- > Planned and executed multiple lines in investigation to meet deadlines. > Set up Journal Club for the group and presentations for the organoid meeting > Generated and analyzed fluorescent reporters (different approaches/techniques) > Optimized and wrote my own protocols. > Prepared data in digital lab book: elabjournal. RELEVANT TECHNIQUES ----------------------------------------------------------- ~ Intestinal Organoid culture ~ Confocal microscope ~ Analyzed development of organoids from one single stem cell ~ Cloning for CRISPaint plasmids ~ Complementation-reporter of fluorescent protein Achilles ~ Llamatag reporter ~ Organoid electroporation ~ Cell culture (3T3 fibroblasts) ~ ImageJ analysis Show less
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The Netherlands Cancer Institute
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Netherlands
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Research Services
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400 - 500 Employee
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Research Internship - Master thesis
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Feb 2019 - Dec 2019
Lab of Dr. William Faller, Junior Group Leader Research area: RNA translation and Cancer Oncogenomics department Project: Understanding the functionality of cyclin D2 in the intestinal crypt-villus using intestinal organoids KEY ACHIEVEMENTS ----------------------------------------------------------- > Planned and executed multiple lines in investigation to meet deadlines. > Set up Journal Clubs for the group and ordered lab material when colleague was on holiday. > Took effectively control of the project after my supervisor unexpectedly left the group. > Generated and synthesized large quantities of data. > Optimized and wrote protocols that are still be used in the lab nowadays. > Organized and planned additional experiments with collaborators in different labs. > Prepared concise and clear written data in lab book and thesis that is reliable and will be used for scientific publication. > Presented data in lunch meeting to Peter Sicinski (Dana-Farber Cancer Institute, Cell-cycle researcher). RELEVANT TECHNIQUES ----------------------------------------------------------- ~ Intestinal Organoid culture ~ Colony forming assay (analyzing single stem cells) ~ qRT-PCR ~ Western Blot ~ Seahorse mitochondrial stress test ~ DNA lysis ~ Resazurin assay ~ Immunofluorescence (IF) ~ RNAscope (Performed at Amsterdam UMC) ~ FACS (Edu Click-it proliferation assay performed at Amsterdam UMC) ~ Immunoprecipitation (IP) ~ Immunohistochemistry (IHC) quantification Show less
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Leiden Science - Universiteit Leiden
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Netherlands
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Higher Education
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100 - 200 Employee
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Research Internship - Bachelor Thesis
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Apr 2018 - Aug 2018
Lab of Prof. Dr. Mario van der Stelt Research area: Molecular physiology Leiden institute of Chemistry Project: Cloning, expression and characterization of engineered kinases for general chemical genetics strategies. KEY ACHIEVEMENTS ----------------------------------------------------------- > Involved and pitched ideas in research group meetings. > Effectively working, clear lab notes and data that was reliable and could be used for publishing. > Comprehended background of advanced kinase research and collaborated on the completion of data used in supervisor’s paper published in Nature Communications. RELEVANT TECHNIQUES ----------------------------------------------------------- ~ Cell culture (Hek293T and U2Os) ~ Gateway recombination ~ Primer design, digestion, ligation and cloning in E.coli ~ PCR ~ Plasmid isolation ~ Cell lysis ~ Covalent probe labeling in SDS-gel ~ Target engagement ~ Click chemistry ~ Western Blot Show less
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Education
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2018 - 2021
Universiteit Leiden
Master of Science - MS, Life science and Technology -
2017 - 2018
Erasmus University Rotterdam
Minor, Geneeskunde -
2015 - 2018
Delft University of Technology
Bachelor's degree, Life science & technology -
2015 - 2018
Leiden University
Bachelor's degree, Life science & technology -
2009 - 2015
Maaslandcollege
VWO-tt, Natuur & techniek
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