Experience

Woodstock Sterile Solutions

• United States
• Pharmaceutical Manufacturing
• 100 - 200 Employee

• Sr. Microbiologist

  • Dec 2020 - Present

  Description Pending Description Pending



Catalent Pharma Solutions

• United States
• Pharmaceutical Manufacturing
• 700 & Above Employee

• Microbiologist II

  • Dec 2014 - Present

  General Duties: - Proficient in sterility, bioburden, endotoxin (gel clot, turbidimetric, and chromogenic), growth promotion, and microbial identification (gram stain and dna sequencing) test methods and method validations. - Executor of media reads and reconciliation, growth promotion of both liquid and plated media types, and compressed gas and steam sampling in Grade A/B Manufacturing Suites. - Investigation author and/or co-author for the following types: bioburden OOS, sterility OOS, objectionable organism recovery, viable and non-viable environmental onitoring OOS, miscellaneous Manufacturing related deviations, etc. - Has been the change control sponsor for biennial reviews, onboarding of new equipment, and process improvements. Additional Duties and Responsibilities: - Qualified Internal Auditor for Catalent, Woodstock. , designated sterility SME, protocol writing for method validations, EMPQs on sterility isolator systems, and equipment qualification. - Onboarding of New Equipment for 21 CFR compliance and building redundant systems - Frequent Trainer for: Sterility, Bioburden, Microbial Identification, Media Fill Reads, and Growth Promotion in addition to the “Aseptic University” series of lectures and hands on training to qualify employees to perform Grade A/B gowning. Miscellaneous Accomplishments: - Gown Certified to perform work in grade A/B spaces - Attended PDA Training for Aseptic Practices December 2017 - Site representative at Catalent, Morrisville as Sterility SME (September 17-October 5th, 2018) and Quality/Technical Reviewer (September 16th to September 27th, 2019) Show less



WILD Flavors, Inc.

• Micro Lab Technician

  • 2008 - 2014

  Inspection and testing of raw materials, finished product, and the plant environment for microbial content and risk assessment including but not limited to bacteria, yeast, mold, and environmental listeria. Verified sanitation cleaning practices through ATP testing, visual inspection, and microbial testing using a "zone" system to assign risk potential. Did statistical analysis of environmental data as part of trend analysis and to verify efficacy of the sanitation program as a OPRP. In addition, I was a member of the Food Safety Team from 2011 to current, participated in audits, helped prep the plant for ISO 9000 certification and helped train others in format and also in maintaining these records (specifically for Micro and QA labs). Did all purchasing of supplies for both micro and QA and kept track of pricing and total monthly spenditures to ensure lab stayed within designated budgets. Show less



Cornell University

• United States
• Higher Education
• 700 & Above Employee

• Research Assistant

  • Aug 2005 - Jul 2006

  - John T Lis lab: Preliminary work was begun to amplify an RNA Aptamer which had previously been shown to biochemically block TATA binding protein (TBP) mediated transcription using PCR and SDS-PAGE. - William Brown's lab: Began preliminary work on identifying the origin of Lipid bodies/fat micelles by loading HeLa cells with an excess of oleic acid. The project goal was to optimize viewing conditions of lipid body formation with a scanning disc confocal microscope. - John T Lis lab: Preliminary work was begun to amplify an RNA Aptamer which had previously been shown to biochemically block TATA binding protein (TBP) mediated transcription using PCR and SDS-PAGE. - William Brown's lab: Began preliminary work on identifying the origin of Lipid bodies/fat micelles by loading HeLa cells with an excess of oleic acid. The project goal was to optimize viewing conditions of lipid body formation with a scanning disc confocal microscope.



University of Illinois Urbana-Champaign

• United States
• Higher Education
• 700 & Above Employee

• Research Lab Technician

  • Jun 2002 - Dec 2004

  - Used sterile technique to maintain human growth fibroblast cells for infection by various parasites and general lab use. - Cryopreservation of various mutant parasite samples and/or molecularly mutated yeast and bacteria via the SPF (slow programmable freezing) technique using liquid nitrogen. - Created, subcloned, and maintained several Toxoplasma gondii and Trypanosoma brucei strains. - Assisted Dr. Shuhong Luo's research on Ca2+-ATPase, an energy dependent transport channel. - Assisted Dr. Silvia N.J. Moreno (Principal Investigator) and Dr. Peter Rohloff in a large-scale comparison of several T. gondii pyrophosphatase knockout mutants. - Continued Dr. Luo’s research on a putative soluble pyrophosphatase by conducting preliminary localization studies, biochemical analysis, and enzymatic assays of said protein. - Presented research results at various conferences and symposiums Luo S., Rholoff P., Cox J., Uyemura S. A., Docampo R.: Trypanosoma brucei plasma membrane-type Ca2+-ATPase 1 (TbPMC1) and 2 (TbPMC2) genes encode functional Ca2+-ATPases localized to the acidocalcisomes and plasma membrane, and essential for Ca2+ homeostasis and growth. J Biol Chem 279: 14427-14439 (2004). Show less



Pierce Biotechnology

• Biotechnology
• 1 - 100 Employee

• Intern

  • May 2003 - Aug 2003

  Aiding in the work of two projects. THe first was the efficient attachment of biotin to antibodies and the improved recovery yield of biotinylated antibodies for alter use. The second was the creation of both yeast and bacterial subspeies that can product Protein L and the purification of said protein from small and large scale cultures to determine if production yields could be increased. Direct contribution to the project was the development of an ELISA assay to verify functionality of purified protein. Strachan E., Mallia K. A., Cox J. M., Antharavally B., Desai S., Sykaluk L., O’Sullivan V., Bell P. A.: Solid-phase biotinylation of antibodies. J. Mol. Recognit. 17: 268-276 (2004). Show less