Joan Russo
Técnico de Laboratorio de Investigación at Cima Universidad de Navarra- Claim this Profile
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Bio
Experience
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Cima Universidad de Navarra
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Spain
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Research Services
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100 - 200 Employee
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Técnico de Laboratorio de Investigación
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Oct 2020 - Present
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Royal Devon & Exeter Hospital
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United Kingdom
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Medical Practices
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1 - 100 Employee
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Medical Laboratory Technician
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Mar 2017 - May 2020
Medical Laboratory Technician in the Immunology, Haematology and Blood Transfusion departments. My duties are: temperature monitoring; analysers’ reagent level monitoring; deep-cleaning and maintenance of laboratory and analysers; maximization of sample workflow by collecting samples from reception area, prioritizing urgent samples and samples with outstanding tests; performing audits and quality control assessments; communication of urgent results to medical staff; and covering nightshifts… Show more Medical Laboratory Technician in the Immunology, Haematology and Blood Transfusion departments. My duties are: temperature monitoring; analysers’ reagent level monitoring; deep-cleaning and maintenance of laboratory and analysers; maximization of sample workflow by collecting samples from reception area, prioritizing urgent samples and samples with outstanding tests; performing audits and quality control assessments; communication of urgent results to medical staff; and covering nightshifts providing out-of-hours service for Haematology and Blood Transfusion departments. Specific duties to Haematology department: maintenance and sample loading of Beckman & Coulter® DxH800 Haematology Analyser and Werfen® ACL TOP 750 CTS Analyser; performing manual tests such as blood films, glandular fever test, malaria PCR-based testing, malaria blood film staining, sickle cell test and bone marrow iron-staining. Currently transitioning to Sysmex® NX900 and SP-50 analysers. Specific duties to Immunology Department: maintenance and sample processing using Werfen® Quanta-Lyser 2, ThermoFisher® scientific Phadia 250 and BD FACSCalibur™; and manual ELISA assays for detection of autoimmune antibodies against skin and endomysium. Specific to Blood Transfusion Department: maintenance and sample processing using Immucor® NEO Automated Blood Testing Analyser; monitoring and ordering blood components using NHS Online Blood Ordering System as per demand; dealing with challenging telephone request some of which require routine or urgent issuing of blood components to patients.
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Medical Laboratory Assistant
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Sep 2016 - Mar 2017
Medical Laboratory Assistant at the Blood Sciences department with UKAS accreditation. My duties were: reception, identification and acceptance of samples in accordance with local and NHS policies; booking in of samples and clinical detail in electronic systems; dealing with telephone enquiries and communication of results to medical staff; and cleaning and maintenance of laboratory equipment and facilities.
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University of Exeter
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United Kingdom
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Higher Education
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700 & Above Employee
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MSc Research Project
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Jan 2019 - Aug 2019
Titled “Integration of functional and structural analyses in the interpretation of missense variants in KCNQ family”. Data-driven project which consisted in comparative analysis of missense variants for all human KCNQ family members using Xenopus laevis KCNQ1 protein (the only available protein model in any specie for KCNQ). Once structural and functional conservation was determined, statistical analysis of pathological missense variants revealed accumulation of pathological missense variants… Show more Titled “Integration of functional and structural analyses in the interpretation of missense variants in KCNQ family”. Data-driven project which consisted in comparative analysis of missense variants for all human KCNQ family members using Xenopus laevis KCNQ1 protein (the only available protein model in any specie for KCNQ). Once structural and functional conservation was determined, statistical analysis of pathological missense variants revealed accumulation of pathological missense variants in such structurally and functionally conserved regions compared to non-pathological missense variants, even revealing ‘hotspot’ amino-acid sites. 3D visualization of these ‘hotspot’ amino acid sites in X. laevis KCNQ1 protein model using PyMOL provided useful insight of the pathogenic effect they might have at a functional level. Show less Titled “Integration of functional and structural analyses in the interpretation of missense variants in KCNQ family”. Data-driven project which consisted in comparative analysis of missense variants for all human KCNQ family members using Xenopus laevis KCNQ1 protein (the only available protein model in any specie for KCNQ). Once structural and functional conservation was determined, statistical analysis of pathological missense variants revealed accumulation of pathological missense variants… Show more Titled “Integration of functional and structural analyses in the interpretation of missense variants in KCNQ family”. Data-driven project which consisted in comparative analysis of missense variants for all human KCNQ family members using Xenopus laevis KCNQ1 protein (the only available protein model in any specie for KCNQ). Once structural and functional conservation was determined, statistical analysis of pathological missense variants revealed accumulation of pathological missense variants in such structurally and functionally conserved regions compared to non-pathological missense variants, even revealing ‘hotspot’ amino-acid sites. 3D visualization of these ‘hotspot’ amino acid sites in X. laevis KCNQ1 protein model using PyMOL provided useful insight of the pathogenic effect they might have at a functional level. Show less
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Biotools B&M Labs, S.A.
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Spain
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Biotechnology
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1 - 100 Employee
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Logistic and Production Assistant
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Apr 2014 - Nov 2014
As the Logistic and Production Assistant my duties were: reception of customers’ orders; preparation and packaging of products from ready-to-use stock and generating orders’ documentation via SAP®; reception and organization of stock in the warehouse; cleaning and maintenance of equipment and facilities, including pressurised room for sterile reagent production. As the Logistic and Production Assistant my duties were: reception of customers’ orders; preparation and packaging of products from ready-to-use stock and generating orders’ documentation via SAP®; reception and organization of stock in the warehouse; cleaning and maintenance of equipment and facilities, including pressurised room for sterile reagent production.
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Centro de Biología Molecular Severo Ochoa
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Cantoblanco, Madrid.
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Research Laboratory Technician
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Jan 2014 - Jul 2014
As a Laboratory Technician in the microbiology team led by Jose Berenguer and Aurelio Hidalgo my duties were: preparation and sterilization of buffers, chemical solutions and liquid/solid culture media; cleaning and maintenance of laboratory equipment and facilities; reception and organization of stock; supporting main research projects by carrying out molecular biology techniques such as: DNA/RNA extraction from bacteria cultures and DNA/RNA purification from agarose gel; primer design and PCR… Show more As a Laboratory Technician in the microbiology team led by Jose Berenguer and Aurelio Hidalgo my duties were: preparation and sterilization of buffers, chemical solutions and liquid/solid culture media; cleaning and maintenance of laboratory equipment and facilities; reception and organization of stock; supporting main research projects by carrying out molecular biology techniques such as: DNA/RNA extraction from bacteria cultures and DNA/RNA purification from agarose gel; primer design and PCR amplification with T. aquaticus DNA polymerase and P. furiosus DNA polymerase for high fidelity amplification; determination of DNA/RNA concentration and quality control parameters; as well DNA electrophoresis, southern blot and SDS-PAGE electrophoresis. Show less As a Laboratory Technician in the microbiology team led by Jose Berenguer and Aurelio Hidalgo my duties were: preparation and sterilization of buffers, chemical solutions and liquid/solid culture media; cleaning and maintenance of laboratory equipment and facilities; reception and organization of stock; supporting main research projects by carrying out molecular biology techniques such as: DNA/RNA extraction from bacteria cultures and DNA/RNA purification from agarose gel; primer design and PCR… Show more As a Laboratory Technician in the microbiology team led by Jose Berenguer and Aurelio Hidalgo my duties were: preparation and sterilization of buffers, chemical solutions and liquid/solid culture media; cleaning and maintenance of laboratory equipment and facilities; reception and organization of stock; supporting main research projects by carrying out molecular biology techniques such as: DNA/RNA extraction from bacteria cultures and DNA/RNA purification from agarose gel; primer design and PCR amplification with T. aquaticus DNA polymerase and P. furiosus DNA polymerase for high fidelity amplification; determination of DNA/RNA concentration and quality control parameters; as well DNA electrophoresis, southern blot and SDS-PAGE electrophoresis. Show less
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Centro de Biología Molecular Severo Ochoa
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Cantoblanco, Madrid
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Bachelor of Science Research Project
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Jan 2013 - Jul 2013
Titled "Statistical analysis of prokaryotic Argonaute protein implication in bacterial transformation efficiency of Thermus thermophilus". Techniques used: bacterial growth in selective and non-selective solid/liquid culture media, DNA extraction, plasmid insert ligation, bacterial transformation, DNA electrophoresis, colony forming-unit count and statistical analysis using Fisher distribution, ANOVA table and Bonferroni correction. Titled "Statistical analysis of prokaryotic Argonaute protein implication in bacterial transformation efficiency of Thermus thermophilus". Techniques used: bacterial growth in selective and non-selective solid/liquid culture media, DNA extraction, plasmid insert ligation, bacterial transformation, DNA electrophoresis, colony forming-unit count and statistical analysis using Fisher distribution, ANOVA table and Bonferroni correction.
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Centro de Biología Molecular Severo Ochoa
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Cantoblanco, Madrid.
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Research Assistant
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Jun 2012 - Aug 2012
Research assistant in a microbiology laboratory specialised in thermophilic microorganisms such as Thermus thermophilus. The aim of the External Practical (optional university module) was to obtain a Escherichia coli strain and Thermus thermophilus strain transformed with T. thermophilus DNA polymerase gene to maximize the production of T. thermophilus DNA polymerase and purify it for posterior enzymatic assays. Techniques used: primer design, PCR amplification, plasmid insert ligation… Show more Research assistant in a microbiology laboratory specialised in thermophilic microorganisms such as Thermus thermophilus. The aim of the External Practical (optional university module) was to obtain a Escherichia coli strain and Thermus thermophilus strain transformed with T. thermophilus DNA polymerase gene to maximize the production of T. thermophilus DNA polymerase and purify it for posterior enzymatic assays. Techniques used: primer design, PCR amplification, plasmid insert ligation, bacterial transformation, bacterial growth on selective media, SDS-PAGE electrophoresis and his-tag protein purification. Show less Research assistant in a microbiology laboratory specialised in thermophilic microorganisms such as Thermus thermophilus. The aim of the External Practical (optional university module) was to obtain a Escherichia coli strain and Thermus thermophilus strain transformed with T. thermophilus DNA polymerase gene to maximize the production of T. thermophilus DNA polymerase and purify it for posterior enzymatic assays. Techniques used: primer design, PCR amplification, plasmid insert ligation… Show more Research assistant in a microbiology laboratory specialised in thermophilic microorganisms such as Thermus thermophilus. The aim of the External Practical (optional university module) was to obtain a Escherichia coli strain and Thermus thermophilus strain transformed with T. thermophilus DNA polymerase gene to maximize the production of T. thermophilus DNA polymerase and purify it for posterior enzymatic assays. Techniques used: primer design, PCR amplification, plasmid insert ligation, bacterial transformation, bacterial growth on selective media, SDS-PAGE electrophoresis and his-tag protein purification. Show less
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Education
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University of Exeter
Master, Genome Sciences/Genomics -
Universidad Autónoma de Madrid
Grado en Biología, Biología Molecular y Celular