JJ Hum
Assay Development Scientist at Boreal Genomics- Claim this Profile
Click to upgrade to our gold package
for the full feature experience.
Topline Score
Bio
Experience
-
Boreal Genomics
-
Canada
-
Biotechnology Research
-
1 - 100 Employee
-
Assay Development Scientist
-
Nov 2021 - Present
• Performed research/development work on Natera’s linked target capture (LTC) technology, a fast and effective alternative to classic hybridization capture for targeted sequencing • Designed LTC assay architectures for duplex capture sequencing • Contributed to development and final performance evaluation of a novel diagnostic liquid comprehensive genomic profiling assay, including LTC library preparation and summary statistics analysis for 305 samples • Investigated LTC assay workflow improvements involving PCR parameter optimization and enzyme additions
-
-
-
BC Centre for Disease Control
-
Canada
-
Hospitals and Health Care
-
200 - 300 Employee
-
Public Health Technologist - Mycobacteriology
-
Mar 2021 - Nov 2021
• Processed clinical samples, prepared microscopy slides, set up liquid and solid cultures• Performed nucleic acid extraction and real-time PCR• Improved lab efficiency by writing Microsoft Excel VBA macros for formatting and interpreting real-time PCR results, and for summarizing production period statistics
-
-
Public Health Technologist - Molecular Services
-
Jul 2020 - Mar 2021
• Designed and evaluated real-time multiplex PCR assays for detection of SARS-CoV-2 variants• Performed COVID-19 swab/media validation studies including design and analysis of viral stability experiments, sterility experiments, and writeup of validation reports• Evaluated performance of commercial SARS-CoV-2 nucleic acid assays and antigen assays• Managed inventory and performed quality control for PCR reagents• Participated in COVID-19 response team:o Performed nucleic acid extraction and real-time PCR on clinical sampleso Performed Illumina whole genome sequencing on clinical samples
-
-
-
BC Centre for Disease Control
-
Canada
-
Hospitals and Health Care
-
200 - 300 Employee
-
Co-op Student
-
May 2019 - Aug 2019
• Performed nucleic acid extraction and real-time PCR on blood samples • Designed a real-time PCR assay for multiplex detection of Ehrlichia and Anaplasma species in tick midgut and mouse tissue samples, evaluated assay performance on clinical samples and drafted a validation report o Abstract and poster presentation were accepted to the 2020 AMMI-CACMID Annual Conference • Performed nucleic acid extraction and real-time PCR on blood samples • Designed a real-time PCR assay for multiplex detection of Ehrlichia and Anaplasma species in tick midgut and mouse tissue samples, evaluated assay performance on clinical samples and drafted a validation report o Abstract and poster presentation were accepted to the 2020 AMMI-CACMID Annual Conference
-
-
-
BC Centre for Disease Control
-
Canada
-
Hospitals and Health Care
-
200 - 300 Employee
-
Co-op Student
-
May 2018 - Dec 2018
• Extracted nucleic acids from 10-30+ clinical samples per day, performed Taqman real-time PCR on the extracts, troubleshot unusual results • Effectively coordinated with other technicians to ensure that both clinical runs and research projects were completed in a timely manner • Updated laboratory databases and quality control forms with high accuracy and attention to detail in preparation for review by the Diagnostic Accreditation Program • Drafted a validation report for a new assay, created guides for future co-op students, gave educational presentations • Researched methods for molecular detection of Helicobacter pylori: o Investigated the detection of tetracycline and clarithromycin resistance in various sample types using Taqman and LightCycler real-time PCR o Explored specificity and sensitivity issues with detecting H. pylori in clinical stool samples using Taqman and LightCycler probes
-
-
-
UBC Zoology - Wood Lab
-
Vancouver, Canada Area
-
Research Assistant
-
May 2017 - Sep 2017
• Established an experimental arena for fish behavioural trials by rigorously testing various layouts for salinity and dissolved oxygen levels • Analyzed trial data using Ethovision® XT, GraphPad Prism 7 and Excel • Cared for research fish by maintaining tank salinity and feeding • Created a poster presentation for UBC’s Multidisciplinary Undergraduate Research Conference • Established an experimental arena for fish behavioural trials by rigorously testing various layouts for salinity and dissolved oxygen levels • Analyzed trial data using Ethovision® XT, GraphPad Prism 7 and Excel • Cared for research fish by maintaining tank salinity and feeding • Created a poster presentation for UBC’s Multidisciplinary Undergraduate Research Conference
-
-
Education
-
The University of British Columbia
Bachelor of Science - BS, Honours Biotechnology -
British Columbia Institute of Technology
Diploma, Biotechnology