Jien Nee Tai
Scientist I at Chugai Pharmabody Research- Claim this Profile
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Bio
Experience
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Chugai Pharmabody Research
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Singapore
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Biotechnology
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1 - 100 Employee
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Scientist I
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Jan 2022 - Present
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Senior Research Associate
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Jan 2020 - Dec 2021
Automation unit- Developed and optimized high throughput automated 30mL - 750mL transfection and purification process for antibodies on Biomek automated liquid handler- Characterize antibodies with HPLC-MS, SDS-PAGE, CE-SDS, SEC, and endotoxin testing- Develop automated solutions for other units in CPR- Increase efficiency by constantly challenging status quo and implementing Microsoft Excel VBA in processes- Manage team of 3 lab technicians
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Research Associate
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Nov 2018 - Jan 2020
Protein Production unit- Conducted 30mL to 2L scale transient expression of proteins in mammalian cell lines- Purified antibodies and antigens with high purity using affinity, ion exchange, and size exclusion chromatography on AKTA FPLC systems- Developed purification strategies for difficult proteins- Analyzed proteins with SDS-PAGE, HPLC-SEC, and cIEF
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Research Assistant
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Aug 2018 - Nov 2018
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The Phi Beta Kappa Society
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United States
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Higher Education
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100 - 200 Employee
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Member
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Dec 2015 - Present
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A*STAR - Agency for Science, Technology and Research
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Singapore
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Research Services
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700 & Above Employee
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Analytical Intern
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Aug 2017 - May 2018
• Investigated the glycome of recombinant glycoprotein, alpha-1 antitrypsin, produced in CHO cells for QbD implementation in biologics manufacturing, supervised by Dr. Terry Nguyen-Khuong & Prof. Pauline Rudd • Purified recombinant protein using AKTA FPLC system • Designed & conducted glycomics experiments using novel techniques, analysed with UPLC-MS/MS • Analysed data using Waters UNIFI software & statistical analyses • Investigated the glycome of recombinant glycoprotein, alpha-1 antitrypsin, produced in CHO cells for QbD implementation in biologics manufacturing, supervised by Dr. Terry Nguyen-Khuong & Prof. Pauline Rudd • Purified recombinant protein using AKTA FPLC system • Designed & conducted glycomics experiments using novel techniques, analysed with UPLC-MS/MS • Analysed data using Waters UNIFI software & statistical analyses
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President
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Aug 2015 - May 2016
• Pioneered online handover system to support yearly transitions of executive committees • Liaised with regional leaders to organise annual Midwest Games for over 100 Singaporean students across 6 universities • Collaborated with other international student groups for campus-wide cross-cultural events • Pioneered online handover system to support yearly transitions of executive committees • Liaised with regional leaders to organise annual Midwest Games for over 100 Singaporean students across 6 universities • Collaborated with other international student groups for campus-wide cross-cultural events
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Undergraduate Research Assistant
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Sep 2013 - May 2016
• Investigated ACAT-inhibiting drug, Avasimibe, as a clinically-viable long-term treatment for currently untreatable prostate cancer, supervised by Dr. Ji-Xin Cheng • Completed independent Honours thesis on combination therapies for castration-resistant prostate cancer • Performed in vitro experiments on prostate cancer cell lines & in vivo experiments with tumour xenograft mouse models • Worked in multicultural and cross-disciplinary environment • Investigated ACAT-inhibiting drug, Avasimibe, as a clinically-viable long-term treatment for currently untreatable prostate cancer, supervised by Dr. Ji-Xin Cheng • Completed independent Honours thesis on combination therapies for castration-resistant prostate cancer • Performed in vitro experiments on prostate cancer cell lines & in vivo experiments with tumour xenograft mouse models • Worked in multicultural and cross-disciplinary environment
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Eli Lilly and Company
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United States
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Pharmaceutical Manufacturing
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700 & Above Employee
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Manufacturing and Quality Intern
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Jun 2015 - Aug 2015
• Developed a gas chromatography method for the in-process QC lab that saved 20 man-hours/week • Utilised DOE and statistical analyses to streamline and improve laboratory testing process • Authored technical report and presented conclusions to senior management • Worked in high pressure, manufacturing cGMP environment • Developed a gas chromatography method for the in-process QC lab that saved 20 man-hours/week • Utilised DOE and statistical analyses to streamline and improve laboratory testing process • Authored technical report and presented conclusions to senior management • Worked in high pressure, manufacturing cGMP environment
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Purdue Science Student Council
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United States
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Higher Education
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1 - 100 Employee
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Committee Head, Campus & Community Outreach
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Jan 2014 - Dec 2014
• Organised initiatives on campus for student and community engagement in science • Networked with other campus organisations to promote the College of Science • Organised initiatives on campus for student and community engagement in science • Networked with other campus organisations to promote the College of Science
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Education
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National University of Singapore
Master of Science (MSc), Industrial Chemistry -
Technical University Munich
Master of Science (MSc), Industrial Chemistry -
Purdue University
Bachelor of Science (BS), Chemistry (ACS), Minor in Economics -
University of Leeds
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Burnsville High School, MN
High School