Curation Scientist I

• Apr 2023 - Present

Medical Researcher

• Oct 2020 - Apr 2023

Perform quality control assessment for Next-Generation Sequencing and other molecular diagnostic assays. Classify detected variants according to the internal and professional guidelines. Draft clinical lab report according to internal and professional standards; submit draft reports to a laboratory director for final review and sign-out. Review literature and databases to evaluate gene-disease association. Review and update genetic variations based on new information.

Lab Technician II

• Mar 2020 - Oct 2020

Prepare COVID-19 clinical samples for an FDA-authorized RT-PCR test and an NGS test in a BSL-2 laboratory. Manage the DNA extraction team to operate smoothly by establishing and troubleshooting procedures, maintaining equipment, and recruiting/training employees. Validate clinical trial testing kits for COVID-19. Perform clinical laboratory test procedures following SOP and QA procedures.

Research Technician I

• Mar 2018 - Feb 2020

Researched on introducing conditional silencing system for Mycobacterium tuberculosis (Mtb) and identification of unknown gene functions. Revised protocol independently and performed genetic modifications and in vivo/in vitro phenotypic screenings. Skills: Multisite Gateway cloning in E. coli for generation of Mtb strains, DNA isolation, DNA sequencing, PCR amplification, gene swapping, recombination, transformation, mutant construction, chromosomal DNA extraction… Show more Researched on introducing conditional silencing system for Mycobacterium tuberculosis (Mtb) and identification of unknown gene functions. Revised protocol independently and performed genetic modifications and in vivo/in vitro phenotypic screenings. Skills: Multisite Gateway cloning in E. coli for generation of Mtb strains, DNA isolation, DNA sequencing, PCR amplification, gene swapping, recombination, transformation, mutant construction, chromosomal DNA extraction, immunoblot, promoter activity assay using reporter systems, growth/spot assay, CFU detection, lipid extraction, thin-layer chromatography, MIC assay, and mouse handling, including Mtb aerosol infections and isolation of organs. Worked proficiently in sterile environments and BSL3/ABSL3 facilities. Prepared and maintained detailed records, and summarized reports of all procedures and results, including graphs, scientific calculations, and statistical analysis charts. Trained new employees. Managed laboratory consumables and equipment and organized lab environments. Show less Researched on introducing conditional silencing system for Mycobacterium tuberculosis (Mtb) and identification of unknown gene functions. Revised protocol independently and performed genetic modifications and in vivo/in vitro phenotypic screenings. Skills: Multisite Gateway cloning in E. coli for generation of Mtb strains, DNA isolation, DNA sequencing, PCR amplification, gene swapping, recombination, transformation, mutant construction, chromosomal DNA extraction… Show more Researched on introducing conditional silencing system for Mycobacterium tuberculosis (Mtb) and identification of unknown gene functions. Revised protocol independently and performed genetic modifications and in vivo/in vitro phenotypic screenings. Skills: Multisite Gateway cloning in E. coli for generation of Mtb strains, DNA isolation, DNA sequencing, PCR amplification, gene swapping, recombination, transformation, mutant construction, chromosomal DNA extraction, immunoblot, promoter activity assay using reporter systems, growth/spot assay, CFU detection, lipid extraction, thin-layer chromatography, MIC assay, and mouse handling, including Mtb aerosol infections and isolation of organs. Worked proficiently in sterile environments and BSL3/ABSL3 facilities. Prepared and maintained detailed records, and summarized reports of all procedures and results, including graphs, scientific calculations, and statistical analysis charts. Trained new employees. Managed laboratory consumables and equipment and organized lab environments. Show less

Graduate Student Research Assistant

• Nov 2016 - Dec 2017

Completed Master’s Thesis: Characterizing the Function of LprG and Ag85A Involved in Mycobacterial Outer Membrane Biosynthesis. Researched in identification of proteins related to lipid transport of Mycobacterium Smegmatis (Msm) and mechanism of outer membrane biogenesis. Established confocal/epifluorescence microscopy protocols and trained an undergraduate research volunteer. Analyzed protein localization, growth rate, and outer membrane phenotype using a… Show more Completed Master’s Thesis: Characterizing the Function of LprG and Ag85A Involved in Mycobacterial Outer Membrane Biosynthesis. Researched in identification of proteins related to lipid transport of Mycobacterium Smegmatis (Msm) and mechanism of outer membrane biogenesis. Established confocal/epifluorescence microscopy protocols and trained an undergraduate research volunteer. Analyzed protein localization, growth rate, and outer membrane phenotype using a biochemical labeling method. Performed microscopic imaging analyses with Excel, ImageJ, and Oufti. Provided data that was published.

Graduate Student Research Assistant

• Sep 2016 - Nov 2016

Contributed to research for central neural mechanisms in normoxia and hypoxia respiratory control and respiratory rhythm and pattern. Performed experiments on mice, including anesthesia, recording the respiratory rates and patterns and isolation of organs.

Research Assistant

• Aug 2013 - Dec 2013

Contributed to a study for investigating epitopes inducing Korean clade HIV-1 common neutralizing antibodies. Assisted with molecular biology techniques, including DNA isolation, DNA electrophoresis, PCR amplification, maintaining cultured cells, transfection and transformation. Contributed to a study for investigating epitopes inducing Korean clade HIV-1 common neutralizing antibodies. Assisted with molecular biology techniques, including DNA isolation, DNA electrophoresis, PCR amplification, maintaining cultured cells, transfection and transformation.