Jonathan Klonowski

Biotechnology Consultant at Special Competitive Studies Project - SCSP
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Chicago, Illinois, United States, US

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Experience

    • United States
    • Research Services
    • 1 - 100 Employee
    • Biotechnology Consultant
      • Sep 2023 - Present

      Fleshing out the details in SCSP's "National Action Plan for U.S. Leadership in Biotechnology," a biotechnology policy roadmap for a coordinated effort among academia, the private sector, and government to establish U.S. leadership in this critical technology through 2030, alongside our allies and partners, from a national security perspective. Fleshing out the details in SCSP's "National Action Plan for U.S. Leadership in Biotechnology," a biotechnology policy roadmap for a coordinated effort among academia, the private sector, and government to establish U.S. leadership in this critical technology through 2030, alongside our allies and partners, from a national security perspective.

    • President Emeritus
      • Jan 2023 - Present

    • President
      • Jul 2020 - Jan 2023

    • Board Member
      • Sep 2018 - Jul 2020

      Using open minded, inclusive and evidence based research, science policy bridges the gap between scientific discoveries and its impact on our society. PSPG’s aim is to be an impartial outlet for people to understand, analyze, and utilize science as a means to impact society. As a group of professional biomedical students, this often means the communication of science through writing for non-scientific audiences and advocacy for both graduate students and science and as a whole.

    • United States
    • Higher Education
    • 700 & Above Employee
    • 2022-2023 PCGC and CDDRC Research Fellow
      • Nov 2022 - Present

      2022-2023 Pediatric Cardiac Genomics Consortium and Cardiovascular Development Data Resource Center Research Fellow: Role of Nonsense-Mediated mRNA Decay (NMD) Escaping Variants in the Pathogenesis of Congenital Heart Disease.

    • Graduate Student Researcher
      • Jan 2018 - Present

      Studying how patients with Congenital Heart Disease (CHD) have ciliary and Hedgehog signaling defects that may have contributed to CHD pathogenesis using a combination of Molecular Biology, Cell Biology, Genomics, and Systems Biology approaches.

    • Ruth L. Kirschstein National Research Service Award (F31) Fellow
      • Apr 2020 - Nov 2022

      Project Title: Derepression of Hedgehog Signaling in Congenital Heart Disease Patients

    • Graduate Rotation Student
      • Jul 2017 - Jan 2018

      Integrative Systems Biology Program:Focused on teaching bioinformatical approaches to the life sciences in order to address disease in a way that is in tune with the clinician's outlook, in order to spark meaningful translational research.

    • United States
    • Public Policy Offices
    • 1 - 100 Employee
    • Diversity, Equity, and Inclusion Committee
      • Mar 2019 - Jan 2023

      The National Science Policy Network's mission is to catalyze the engagement of early career scientists and engineers in policy making by training the next generation of leaders, fostering community, and advocating for the role of science in policy. The National Science Policy Network's mission is to catalyze the engagement of early career scientists and engineers in policy making by training the next generation of leaders, fostering community, and advocating for the role of science in policy.

    • United States
    • Political Organizations
    • Member
      • Dec 2018 - Jul 2020

      Student Advocates for Graduate Education (SAGE) is an entirely student run organization dedicated to representing graduate and professional students across the nation. SAGE is comprised of member groups from public research universities across the nation. The mission of Student Advocates for Graduate Education (SAGE) is to: IMPROVE the quality of graduate student life at their own campuses. PROMOTE access, quality, and opportunity for graduate and professional students at the federal level. Show less

    • United States
    • Hospitals and Health Care
    • 700 & Above Employee
    • Research Assistant
      • Jan 2017 - Jun 2017

      ¥ Experimentation with Vero Cell line. Maintenance of Vero cell lines and using them for experiments such as plaque assays, infections, transfections, and CrisprCas9 experiments. ¥ Establishment of Crispr technology for the laboratory. Rational design of and use of HDR mediated knockouts for inquiries into coronavirus infection. ¥ Employment of fluorescence microscopy. Immunofluorescent labeling of cells, observing results under a fluorescent microscope and analyzing the data. ¥ Studying Avian Infectious Bronchitis Virus and SARS proteins as a models for the study of coronaviruses. ¥ Observing the operation of Flow Cytometry and Electron Microscope. Show less

    • United States
    • Hospitals and Health Care
    • 700 & Above Employee
    • Research Assistant
      • Nov 2015 - Mar 2016

      • DNA extraction, amplification, isolation, insertion into plasmids with standard ligation and LR reactions. • Expression and isolation of proteins via bacterial cell transformation, IPTG induced protein expression, cell lysis via sonication or French press, and isolation via column chromatography or magnetic beads. • Culturing Hela cell and HEK293T cells. Including cell counting and cell lysis in addition to exposure to transfection, siRNA knockdown, and CRISPR/Cas9 knockout. • Practice of Western blotting to analyze disturbances in relative protein concentrations. Including Coomassie blue staining, stripping western blots, ELC luminal-based detection, and exposures. • Examining the roll of proteins in a Zebrafish model. Consisting of zebrafish husbandry, embryo collection, microinjection of embryos with Morphalino or CRISPR/Cas9, and subsequent documentation of development. • Maintenance of liquid nitrogen storage tank, preparation of reagents for the entire laboratory and other shared laboratory upkeep duties. Show less

    • Research Assistant
      • Aug 2014 - Jun 2015

      • Utilized biochemical methods and cell-based assays to expand knowledge of proprotein convertase proteolytic mechanisms and their relation to Human Papilloma Virus. • Created genetically engineered recombinant proteins, transfected their genes into cells and over-expressed the protein using virus in order to create stock protein for kinetic analysis. • Isolated recombinant proteins through high-pressure filtration, silica gel column, gel column, high performance liquid chromatography, and ion exchange chromatography for HPV based assays. • Employed a spectrophotometer to monitor kinetic behavior, with and without the presence of inhibitors, of five different proprotein converses and mutants exercising fluorescence marker substrates to kinetically characterize these proteins. • Constructed HPV assays consisting of Human Embryonic Kidney Cells (HEK293T) and HPV psudovirions with a green fluorescence reporter gene to clarify the role of each proprotein convertase in respects to HPV cell entry. • Performed laboratory calculations, prepared solutions/dilutions and cultured bacterial, insect, and human cells. Show less

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