Experience

Gatehouse Bio

• United States
• Biotechnology Research
• 1 - 100 Employee

• Research Associate II

  • Apr 2022 - Present

• Research Associate II

  • Apr 2022 - Present



Brigham and Women's Hospital

• United States
• Hospitals and Health Care
• 700 & Above Employee

• Research Technician II

  • Sep 2016 - Apr 2022

  Characterization of the novel filament-forming stress-inducible protein Yor289w • Treated Saccharomyces cerevisiae with Unfolded Protein Response inducing reagents and measured upregulation by Western blot, RT-PCR, and confocal microscopy The role of Ubp3 in stress-induced gene expression • Mutated putative ubiquitination sites of Arr3 to confirm them via spotting assay and cycloheximide chase assay • Identified a role for Ubp3 in arsenite-induced transcription of Arr2 and… Show more Characterization of the novel filament-forming stress-inducible protein Yor289w • Treated Saccharomyces cerevisiae with Unfolded Protein Response inducing reagents and measured upregulation by Western blot, RT-PCR, and confocal microscopy The role of Ubp3 in stress-induced gene expression • Mutated putative ubiquitination sites of Arr3 to confirm them via spotting assay and cycloheximide chase assay • Identified a role for Ubp3 in arsenite-induced transcription of Arr2 and Arr3 by RT-PCR The molecular basis of supersized lipid droplets (SLDs) • Analyzed the formation of SLDs by making genetic manipulations and imaging with confocal microscopy • Determined the composition of SLDs by thin layer chromatography Show less Characterization of the novel filament-forming stress-inducible protein Yor289w • Treated Saccharomyces cerevisiae with Unfolded Protein Response inducing reagents and measured upregulation by Western blot, RT-PCR, and confocal microscopy The role of Ubp3 in stress-induced gene expression • Mutated putative ubiquitination sites of Arr3 to confirm them via spotting assay and cycloheximide chase assay • Identified a role for Ubp3 in arsenite-induced transcription of Arr2 and… Show more Characterization of the novel filament-forming stress-inducible protein Yor289w • Treated Saccharomyces cerevisiae with Unfolded Protein Response inducing reagents and measured upregulation by Western blot, RT-PCR, and confocal microscopy The role of Ubp3 in stress-induced gene expression • Mutated putative ubiquitination sites of Arr3 to confirm them via spotting assay and cycloheximide chase assay • Identified a role for Ubp3 in arsenite-induced transcription of Arr2 and Arr3 by RT-PCR The molecular basis of supersized lipid droplets (SLDs) • Analyzed the formation of SLDs by making genetic manipulations and imaging with confocal microscopy • Determined the composition of SLDs by thin layer chromatography Show less



Brandeis University

• United States
• Higher Education
• 700 & Above Employee

• Research Assistant

  • Feb 2014 - Aug 2016

  • Monitored levels of autophagy in different strains of yeast under DNA damaging conditions • Organized and presented my results in lab group meetings and in the 2014 Summer SciFest poster session

• Senior thesis

  • Sep 2015 - May 2016

  The role of Hog1 and its regulators in genotoxin-induced targeted autophagy • Characterized the role of Hog1, which was implicated in genotoxin-induced targeted autophagy to shed light on how to regulate the homologous pathways in humans to prevent cancerous cells from developing

• Research Assistant

  • May 2015 - Jul 2015

  Division of Science Summer Undergraduate Research fellowship program Characterization of Pph3 in DNA damage-induced autophagy • Characterized Pph3 in DNA damage-induced autophagy to study how the proteolysis systems affect the ability of Saccharomyces cerevisiae to perform mitosis even with an irreparable DNA double strand break, modeling cancerous cells • Presented my results at the end of summer in the SciFest V poster session