Experience

Integra Therapeutics
• Spain
• Biotechnology Research
• 1 - 100 Employee
• Lab manager at Integra Therapeutics

  • Jan 2022 - Present


PRBB (Barcelona Biomedical Research Park)

• Spain
• Research Services
• 1 - 100 Employee

• • Jul 2018 - Dec 2021

• • Feb 2018 - Jul 2018

  Master thesis in Dr. Marc Güell’s Laboratory on Translational Synthetic Biology at the Universitat Pompeu Fabra collaborating in the mammalian genome editing and gene therapy ongoing project. The research goal is to search for alternatives to precisely re-write genomes safely and efficiently, which can further be applied on disease therapies. Specifically, my master thesis is related on the generation of programmable gene delivery tools based on CRISPR-Cas9 adapted for in vivo gene editing.



VIB-UGent

• Biotechnology Research
• 1 - 100 Employee

• Practicum Student

  • Feb 2017 - Jul 2017

  Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 is a powerful technology for genome editing. To increase the efficiency and ease of recovering edited plants, efforts are focused on optimizing the delivery of CRISPR reagents to plants. The promoter used to drive the expression Cas9 is important for the recovery of heritable mutations in Arabidopsis. The induced-mutation rates using four different constitutive promotors (ZmUbi, RPS5, 35SP and PcUbi) have been evaluated. The gene phytoene desaturase (PDS) of Arabidopsis thaliana, Nicotiana tabacum and N. benthamiana was chosen as PDS mutants are easily recognized by their chlorophyll deficiency. Genomic DNA was extracted and PDS targets were Sanger sequenced and analysed in TIDE to estimate mutation frequency. These results will allow us to determine the optimal promoter for future use. Since the generation of transgenic plants is a relatively slow procedure, results from this analysis will be correlated with ongoing experiments using agro-infiltration and cell cultures to see if these faster and simpler methods are suitable models for determining gene editing efficacy. Show less