Experience

Genesys Diagnostics, Inc

• United States
• Biotechnology Research
• 1 - 100 Employee

• Next Generation Sequencing Technologist/Molecular Lab Technologist

  • Aug 2019 - Present

  Responsible for oversight of all NGS sequencing operations, from accessioning of samples, checking billing status, sample extraction, sample quality control, library preparation, dilution, loading of sequencers & sequencing itself, data management post-run, bioinformatics/variant analysis in Golden Helix visualization software, and final variant interpretation and reporting to Laboratory Sequencing Director. Also responsible for oversight of all aspects related to SARS-CoV2-2019 (COVID-19)… Show more Responsible for oversight of all NGS sequencing operations, from accessioning of samples, checking billing status, sample extraction, sample quality control, library preparation, dilution, loading of sequencers & sequencing itself, data management post-run, bioinformatics/variant analysis in Golden Helix visualization software, and final variant interpretation and reporting to Laboratory Sequencing Director. Also responsible for oversight of all aspects related to SARS-CoV2-2019 (COVID-19) testing, including personnel training, sample accessioning, extraction, testing, resulting, and final case sign-out. ● Collaborated with technical supervisor to ensure proper protocols were followed by all laboratory staff ● Utilized analytical reasoning to fully validate a Cardiology Sequencing panel for Illumina’s NGS platform, including performing all experiments, data analysis, and writing up of a 50 page validation summary; upon completion, lab can now test for inherited cardiac conditions. ● Continued processing of specimens for inherited cancer testing panels. ● Out of necessity, leveraged my ability to learn new responsibilities in order to quickly acquire the necessary knowledge base to both analyze sample variant call files (.vcf files) in Golden Helix software to assist in data analysis, variant calling/visualization, final variant interpretation and writing up of final patient reports for director sign off. ● Along with the rest of the team, have tested approximately 110,000 samples for Covid-19 since mid-March of 2020 utilizing the Luminex NxTag Covid panel and the Luminex MagPix instrument. Show less Responsible for oversight of all NGS sequencing operations, from accessioning of samples, checking billing status, sample extraction, sample quality control, library preparation, dilution, loading of sequencers & sequencing itself, data management post-run, bioinformatics/variant analysis in Golden Helix visualization software, and final variant interpretation and reporting to Laboratory Sequencing Director. Also responsible for oversight of all aspects related to SARS-CoV2-2019 (COVID-19)… Show more Responsible for oversight of all NGS sequencing operations, from accessioning of samples, checking billing status, sample extraction, sample quality control, library preparation, dilution, loading of sequencers & sequencing itself, data management post-run, bioinformatics/variant analysis in Golden Helix visualization software, and final variant interpretation and reporting to Laboratory Sequencing Director. Also responsible for oversight of all aspects related to SARS-CoV2-2019 (COVID-19) testing, including personnel training, sample accessioning, extraction, testing, resulting, and final case sign-out. ● Collaborated with technical supervisor to ensure proper protocols were followed by all laboratory staff ● Utilized analytical reasoning to fully validate a Cardiology Sequencing panel for Illumina’s NGS platform, including performing all experiments, data analysis, and writing up of a 50 page validation summary; upon completion, lab can now test for inherited cardiac conditions. ● Continued processing of specimens for inherited cancer testing panels. ● Out of necessity, leveraged my ability to learn new responsibilities in order to quickly acquire the necessary knowledge base to both analyze sample variant call files (.vcf files) in Golden Helix software to assist in data analysis, variant calling/visualization, final variant interpretation and writing up of final patient reports for director sign off. ● Along with the rest of the team, have tested approximately 110,000 samples for Covid-19 since mid-March of 2020 utilizing the Luminex NxTag Covid panel and the Luminex MagPix instrument. Show less



PerkinElmer

• United States
• Biotechnology Research
• 700 & Above Employee

• Lab Technician

  • Feb 2018 - May 2019

  In this CLIA, CAP-accredited clinical laboratory I conduct multiple procedures including sample quality control, NGS library preparation and sequencing, Sanger sequencing, and MLPA assays, ensuring that our samples meet minimum laboratory requirements, including final quantification, dilution, and producing sample sheets for sequencing runs. Due to the nature of both the laboratory and my own expertise, I am often asked to perform unique assessments, and I always enjoy training my colleagues in… Show more In this CLIA, CAP-accredited clinical laboratory I conduct multiple procedures including sample quality control, NGS library preparation and sequencing, Sanger sequencing, and MLPA assays, ensuring that our samples meet minimum laboratory requirements, including final quantification, dilution, and producing sample sheets for sequencing runs. Due to the nature of both the laboratory and my own expertise, I am often asked to perform unique assessments, and I always enjoy training my colleagues in new laboratory techniques and procedures to expand company-wide skill set and add flexibility. Among my duties is verifying the most critical stage of processing, final quantification, dilution, sample sheet production, and actual sample pooling before a sequencing run, an important role as runs cost an average of $20,000 to $25,000. ● Collaborated with team of four to process and sequence nearly 2000 clinical genetics samples weekly. ● Expanded expertise on new instruments and protocols including “boutique” clinical tests, trained colleagues in newly acquired expertise. ● Performed pilot experiments to validate RNA sequencing using Agilent’s SureSelect Strand-Specific RNA Library prep for multiplexed Illumina sequencing protocol with goal of validating for clinical lab setting. ● Leveraged extensive expertise and excellent written communication skills in authoring or modifying SOPs for a variety of protocols, translating complex procedures into actionable guidance. ● Selected by management to lead visiting team from Australia through full process of technologies, walked team through each stage and responding to questions, receiving very positive feedback from team after tour and training. ● Demonstrated flexibility and multi-tasking when called on to assist or take over from colleague needed elsewhere, readily adapting to new role and requirements. Show less In this CLIA, CAP-accredited clinical laboratory I conduct multiple procedures including sample quality control, NGS library preparation and sequencing, Sanger sequencing, and MLPA assays, ensuring that our samples meet minimum laboratory requirements, including final quantification, dilution, and producing sample sheets for sequencing runs. Due to the nature of both the laboratory and my own expertise, I am often asked to perform unique assessments, and I always enjoy training my colleagues in… Show more In this CLIA, CAP-accredited clinical laboratory I conduct multiple procedures including sample quality control, NGS library preparation and sequencing, Sanger sequencing, and MLPA assays, ensuring that our samples meet minimum laboratory requirements, including final quantification, dilution, and producing sample sheets for sequencing runs. Due to the nature of both the laboratory and my own expertise, I am often asked to perform unique assessments, and I always enjoy training my colleagues in new laboratory techniques and procedures to expand company-wide skill set and add flexibility. Among my duties is verifying the most critical stage of processing, final quantification, dilution, sample sheet production, and actual sample pooling before a sequencing run, an important role as runs cost an average of $20,000 to $25,000. ● Collaborated with team of four to process and sequence nearly 2000 clinical genetics samples weekly. ● Expanded expertise on new instruments and protocols including “boutique” clinical tests, trained colleagues in newly acquired expertise. ● Performed pilot experiments to validate RNA sequencing using Agilent’s SureSelect Strand-Specific RNA Library prep for multiplexed Illumina sequencing protocol with goal of validating for clinical lab setting. ● Leveraged extensive expertise and excellent written communication skills in authoring or modifying SOPs for a variety of protocols, translating complex procedures into actionable guidance. ● Selected by management to lead visiting team from Australia through full process of technologies, walked team through each stage and responding to questions, receiving very positive feedback from team after tour and training. ● Demonstrated flexibility and multi-tasking when called on to assist or take over from colleague needed elsewhere, readily adapting to new role and requirements. Show less



UConn Health

• United States
• Hospitals and Health Care
• 700 & Above Employee

• Lab Manager/Research Assistant II

  • Sep 2015 - Feb 2018

  This position involved managing multiple aspects of daily lab operations including proactive inventory management and prioritizing the general workflow, facilitating effective research procedures by organizing lab setup and consumables. To ensure the utility of animal stock I conducted mouse husbandry activities, monitoring timed matings to assist in gathering embryos to profile for epigenetic marks. In addition, I conducted all basic molecular biology tasks and performed cloning experiments to… Show more This position involved managing multiple aspects of daily lab operations including proactive inventory management and prioritizing the general workflow, facilitating effective research procedures by organizing lab setup and consumables. To ensure the utility of animal stock I conducted mouse husbandry activities, monitoring timed matings to assist in gathering embryos to profile for epigenetic marks. In addition, I conducted all basic molecular biology tasks and performed cloning experiments to insert guide RNAs into plasmids for downstream use in gene editing. ● Advanced research agenda through support of graduate student and post-doctoral fellow research. ● Maintained web-based LIMS to track samples, antibodies, consumables, protocols in addition to accurate records of all experimental activities. ● Utilized kits compatible with Illumina’s MiSeq and Next-Seq platforms for sample library preparation, loaded instruments into quality-controlled libraries. ● Co-authored: “High Resolution Epigenomic Atlas of Craniofacial Development,” published in Cell Rep. 2018 May 1;23(5):1581-1597. doi: 10.1016/j.celrep.2018.03.129. Show less This position involved managing multiple aspects of daily lab operations including proactive inventory management and prioritizing the general workflow, facilitating effective research procedures by organizing lab setup and consumables. To ensure the utility of animal stock I conducted mouse husbandry activities, monitoring timed matings to assist in gathering embryos to profile for epigenetic marks. In addition, I conducted all basic molecular biology tasks and performed cloning experiments to… Show more This position involved managing multiple aspects of daily lab operations including proactive inventory management and prioritizing the general workflow, facilitating effective research procedures by organizing lab setup and consumables. To ensure the utility of animal stock I conducted mouse husbandry activities, monitoring timed matings to assist in gathering embryos to profile for epigenetic marks. In addition, I conducted all basic molecular biology tasks and performed cloning experiments to insert guide RNAs into plasmids for downstream use in gene editing. ● Advanced research agenda through support of graduate student and post-doctoral fellow research. ● Maintained web-based LIMS to track samples, antibodies, consumables, protocols in addition to accurate records of all experimental activities. ● Utilized kits compatible with Illumina’s MiSeq and Next-Seq platforms for sample library preparation, loaded instruments into quality-controlled libraries. ● Co-authored: “High Resolution Epigenomic Atlas of Craniofacial Development,” published in Cell Rep. 2018 May 1;23(5):1581-1597. doi: 10.1016/j.celrep.2018.03.129. Show less



The Rothberg Institute For Childhood Diseases

• Guilford, CT

• Laboratory Manager (Biology), Research Associate

  • May 2011 - Sep 2015

  At the Rothberg Institute I was charged with coordinating lab operations, supervising a staff of four, ensuring our compliance with all relevant safety standards, ordering supplies, directing equipment maintenance, and adhering to project budgets. My research contributions included constructing DNA and RNA libraries that incorporated a variety of approaches aligned with identified experimental objectives, and I processed research and clinical samples and conducted quality control measures… Show more At the Rothberg Institute I was charged with coordinating lab operations, supervising a staff of four, ensuring our compliance with all relevant safety standards, ordering supplies, directing equipment maintenance, and adhering to project budgets. My research contributions included constructing DNA and RNA libraries that incorporated a variety of approaches aligned with identified experimental objectives, and I processed research and clinical samples and conducted quality control measures incorporating sonication methods, monitored project process, and summarized projects in project reports to management. ● Served in field testing role for Ion Torrent technology, exploring limits and utility for assay improvement. ● Constructed IRB approved study investigating genetic basis to performing high-level mathematics, drafted research proposal, recruited project participants, and maintained contact throughout study. ● Conducted multiple reviews of literature to determine optimal methodologies and protocols to achieve research objectives aligned with latest development in the field. Show less At the Rothberg Institute I was charged with coordinating lab operations, supervising a staff of four, ensuring our compliance with all relevant safety standards, ordering supplies, directing equipment maintenance, and adhering to project budgets. My research contributions included constructing DNA and RNA libraries that incorporated a variety of approaches aligned with identified experimental objectives, and I processed research and clinical samples and conducted quality control measures… Show more At the Rothberg Institute I was charged with coordinating lab operations, supervising a staff of four, ensuring our compliance with all relevant safety standards, ordering supplies, directing equipment maintenance, and adhering to project budgets. My research contributions included constructing DNA and RNA libraries that incorporated a variety of approaches aligned with identified experimental objectives, and I processed research and clinical samples and conducted quality control measures incorporating sonication methods, monitored project process, and summarized projects in project reports to management. ● Served in field testing role for Ion Torrent technology, exploring limits and utility for assay improvement. ● Constructed IRB approved study investigating genetic basis to performing high-level mathematics, drafted research proposal, recruited project participants, and maintained contact throughout study. ● Conducted multiple reviews of literature to determine optimal methodologies and protocols to achieve research objectives aligned with latest development in the field. Show less