Jeff Duryea Jr
Research Assistant II at The Jackson Laboratory- Claim this Profile
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Bio
Experience
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The Jackson Laboratory
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United States
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Biotechnology Research
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700 & Above Employee
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Research Assistant II
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Jul 2019 - Present
Working to implement Crispr-cas9 technology at Jax Labs and integrate reporters to track gene editing via homology directed repair and non-homologous end joining, under the direction of Dr. Steve Murray and Dr. Kathy Snow. Working to implement Crispr-cas9 technology at Jax Labs and integrate reporters to track gene editing via homology directed repair and non-homologous end joining, under the direction of Dr. Steve Murray and Dr. Kathy Snow.
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Vanderbilt University School of Medicine
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United States
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Higher Education
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200 - 300 Employee
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Research Assistant III
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May 2011 - Nov 2018
Christopher Wright Lab • Worked on a variety of projects studying mouse and human pancreas organogenesis during fetal, postnatal and juvenile periods to study the pathogenesis of diabetes • Well versed in a variety of techniques aimed at studying pancreatic function and development, such as PCR, immunofluorescence, immunohistochemistry, tissue processing and tissue management, and tissue clearing protocols • Played major role in a resource generation project aimed at the development and characterization of antibodies. Antibodies, generated against difficult antigens, were used by pancreatic research community and commercial enterprises • Maintained and managed multiple mouse colonies for a variety of projects. Characterized mouse lines containing different Cre LoxP inducible systems and developed complex multi-gene mating schemes Show less
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Vanderbilt University
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United States
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Higher Education
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700 & Above Employee
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Research Assistant
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2010 - 2011
Ellen Fanning Lab • Helped support studies on SV40 eukaryotic DNA replication and molecular components involved. Solely responsible for producing fusion proteins used in pull down assays to map the replication fork complex and mapping binding sites of essential proteins • Generated large number of fusion proteins with different tags under various conditions to maximize amount of protein generated from culturing and purification Ellen Fanning Lab • Helped support studies on SV40 eukaryotic DNA replication and molecular components involved. Solely responsible for producing fusion proteins used in pull down assays to map the replication fork complex and mapping binding sites of essential proteins • Generated large number of fusion proteins with different tags under various conditions to maximize amount of protein generated from culturing and purification
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University of Alabama at Huntville
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Huntsville, Alabama Area
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Lab Assistant
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2009 - 2010
Luis Cruz-Vera Lab • Studied ribosomal subunits 30S/50S involvement in translational control within E. coli • Gained understanding of molecular mechanism and components involved in pause effect caused during translation of tryptophan, causing accumulation of intermediary products called peptidyl-tRNAs Luis Cruz-Vera Lab • Studied ribosomal subunits 30S/50S involvement in translational control within E. coli • Gained understanding of molecular mechanism and components involved in pause effect caused during translation of tryptophan, causing accumulation of intermediary products called peptidyl-tRNAs
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University of Alabama at Huntsville
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Huntsville, Alabama Area
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Lab Specialist
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2008 - 2009
Debra Moriarity Lab • In collaboration with UAH Chemistry Department, tested potential of plant extracts, taken from tropical plant species, for therapeutic cancer treatments. • Worked with breast cancer cell lines MCF-7 and MDA-MB-231, plate readers and MTT assays to determine cytotoxicity effects of extracts on cancer cell lines. Debra Moriarity Lab • In collaboration with UAH Chemistry Department, tested potential of plant extracts, taken from tropical plant species, for therapeutic cancer treatments. • Worked with breast cancer cell lines MCF-7 and MDA-MB-231, plate readers and MTT assays to determine cytotoxicity effects of extracts on cancer cell lines.
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Education
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University of Alabama at Huntsville
Bachelor of Science - BS, Molecular Biology