Experience

Hokkaido University

• Japan
• Higher Education
• 400 - 500 Employee

• Assistant Professor

  • Oct 2018 - Present

  My present research work is on understanding DNA gyrase-mediated drug resistance mechanism of fluoroquinolones on M. avium. The mycobacterium fluoroquinolone resistance protein (mfpA) has been shown to protect DNA gyrase from fluoroquinolones and eventually increase the MIC of the fluoroquinolones. I have cloned and expressed M. avium gyrA, gyrB, mfpA WT, and mfpA-frameshift proteins, and will be soon starting DNA supercoiling assay with different fluoroquinolones to understand the role of mfpA proteins in fluoroquinolone resistance to M. avium.Earlier in my two year position in Prof. Hiroyuki Yamaguchi lab at Faculty of Health Sciences, Hokkaido University, I focused on elucidating the molecular mechanism of growth dynamics of Chlamydia trachomatis in hypoxia.

• JSPS Postdoctoral fellow

  • Oct 2017 - Sep 2018

  Developed a simple visual methyl green based dry loop-mediated isothermal amplification method for early detection of Mycobacterium tuberculosis from clinical samples (Thapa et al. Tuberculosis 2019; 117:1-6).



National Trust for Nature Conservation

• Nepal
• Non-profit Organization Management
• 1 - 100 Employee

• Veterinary Officer

  • Jul 2009 - Mar 2013

  Wildlife health management and research works. Wildlife health management and research works.



Tribhuvan University, Institute of Agriculture and Animal Science, Rampur Campus Chitwan

• Nepal
• Education Administration Programs
• 1 - 100 Employee

• Research Associate

  • Dec 2007 - Jun 2009

  I worked in a team of national and international experts for elephant tuberculosis care and management. We developed a Nepal Elephant Tuberculosis Control and Management Action Plan (2011-2015), a first of its kind in elephant range counties https://ntnc.org.np/sites/default/files/doc_publication/2018-11/Nepal%20Elephant%20TB%20Control%20and%20Mgt%20Action%20Plan.pdf. I worked in a team of national and international experts for elephant tuberculosis care and management. We developed a Nepal Elephant Tuberculosis Control and Management Action Plan (2011-2015), a first of its kind in elephant range counties https://ntnc.org.np/sites/default/files/doc_publication/2018-11/Nepal%20Elephant%20TB%20Control%20and%20Mgt%20Action%20Plan.pdf.