Jean de Lima

Master's Degree Student in Immunology at Institute of Biomedical Sciences (ICB-USP)
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Contact Information
us****@****om
(386) 825-5501
Location
Basel, Basel, Switzerland, CH
Languages
  • Portuguese Native or bilingual proficiency
  • English Native or bilingual proficiency
  • Spanish Professional working proficiency
  • French Professional working proficiency

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Credentials

  • Eurpoean Congress of Immunology
    European Federation of Immunological Societies
    Mar, 2020
    - Oct, 2024
  • Nature Conference: Next Gen Immunology 2020
    Weizmann Institute of Science
    Feb, 2020
    - Oct, 2024
  • Flow Cytometry Course
    CHUV / Centre hospitalier universitaire vaudois
    Jan, 2020
    - Oct, 2024
  • Britsh Society Immunology Congress
    British Society for Immunology
    Dec, 2019
    - Oct, 2024
  • Inflamma V: Brazilian Society of Inflammation
    CEPID CRID
    Jun, 2018
    - Oct, 2024
  • Biotechnology Bacherol
    Pontifical Catholic University of Paraná
    Feb, 2018
    - Oct, 2024
  • Practical Course in Molecular Biology of Malignant Transformation
    University of São Paulo
    Jan, 2018
    - Oct, 2024
  • Bioinformatic course - R language course
    Federal Unvesity of Paraná
    Jan, 2016
    - Oct, 2024
  • Course of Tools and Techniques in Molecular Biology
    Pontifical Catholic University of Paraná
    Jul, 2015
    - Oct, 2024

Experience

    • Brazil
    • Higher Education
    • 100 - 200 Employee
    • Master's Degree Student in Immunology
      • Jul 2018 - Present

      The project being carried out is related to the functions of sirtuins in the immune response in the context of obesity and organ transplantation. Technical qualities In vivo models: Skin Transplantation; Diet-induced obesity; DTH; EAE and Melanoma (B16) model. In vitro and ex vivo models: Generation of BMDCS, BMDM and PBMCs; Lymphoid cells culture; Flow Cytometry; ELISA; qPCR; standard PCR; Western Blot; CRISPR assay; Mitotracker assay; Mitosox assay; LC-MS assay and Seahorse XF Cell Mito Stress. Show less

    • Switzerland
    • Research Services
    • 700 & Above Employee
    • Student Internship
      • Sep 2019 - Apr 2020

      The project carried out is related to the investigation of the effect of the genetic deletion of sirtuins by CRISPR-cas9 on dendritic cells. Technical Qualities In vivo models: Skin Transplantation; Diet-induced obesity; DTH; EAE and Melanoma (B16) model. In vitro and ex vivo models: Generation of BMDCS, BMDM and PBMCs; Lymphoid cells culture; Flow Cytometry; ELISA; qPCR; standard PCR; Western Blot; CRISPR assay The project carried out is related to the investigation of the effect of the genetic deletion of sirtuins by CRISPR-cas9 on dendritic cells. Technical Qualities In vivo models: Skin Transplantation; Diet-induced obesity; DTH; EAE and Melanoma (B16) model. In vitro and ex vivo models: Generation of BMDCS, BMDM and PBMCs; Lymphoid cells culture; Flow Cytometry; ELISA; qPCR; standard PCR; Western Blot; CRISPR assay

    • Non-profit Organizations
    • 1 - 100 Employee
    • Student Ambassador
      • Mar 2020 - Mar 2020

      The position of student ambassador was related to the student representative, organizing discussions and promoting speeches at the European Congress on Immunology The position of student ambassador was related to the student representative, organizing discussions and promoting speeches at the European Congress on Immunology

Education

  • Pontifical Catholic University of Paraná
    Biotechnologist, Immunology
    2014 - 2017

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