Head of Cell Biology Group

• Sep 2021 - Present

Project Manager

• May 2022 - Present

Head of Cell and Molecular Biology Group

• Jun 2020 - Sep 2021

Head of Molecular Biology Group

• Nov 2018 - Jun 2020

Molecular Biology Specialist

• Apr 2018 - Nov 2018

Main responsibilities:- Design and execution of cloning strategies for mammalian and prokaryotic expression vectors.- Supervision of cloning in CRO department of the company.- Employment of various cloning techniques and implementation of new solutions to enhance work efficiency.- Design of cloning pipelines and bacterial cloning platform.- Managing molecular biology lab.Core skills:- Cloning using various techniques - restriction, LIC, Gibson Assembly, Golden Gate, RF-cloning and others.- Mutagenesis by site-directed mutagenesis.- Proficiency in Geneious software package.- Project management and small group management (up to 3 people) Show less

Postdoctoral Research Fellow

• May 2016 - Apr 2018

Project: Investigation of ROS formation during mitotic arrest and its impact on cell survival. During my work in School of Medicine Research Group I: • Used various FACS-based measurements of ROS to investigate ROS formation during mitotic arrest. • Examined how ROS accumulation impacts DNA damage response through γH2AX formation using immunofluorescence and FISH techniques. • Investigated the influence of ROS formation during mitotic arrest on cell survival through live-cell imaging and other cell-based assays. Show less

PhD Fellow

• Oct 2011 - Jan 2016

Project: Twist oncogenes drive adaptive response towards oxidative and hypoxic stress. I worked full time in HCV and Cancer group, where I: • Identified molecular mechanism of Twist protection against ROS induced cell death (Floc'h,Kolodziejski et al 2013). • Discovered Twist’s non-cell autonomous, protective effect on cell survival under hypoxia (Kolodziejski et al in preparations) • Trained and mentored 2 students: designed and supervised their research projects. Project: Twist oncogenes drive adaptive response towards oxidative and hypoxic stress. I worked full time in HCV and Cancer group, where I: • Identified molecular mechanism of Twist protection against ROS induced cell death (Floc'h,Kolodziejski et al 2013). • Discovered Twist’s non-cell autonomous, protective effect on cell survival under hypoxia (Kolodziejski et al in preparations) • Trained and mentored 2 students: designed and supervised their research projects.

Voluntary Research Intern

• 2007 - 2011

Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University. I was accepted as a lab member on a competitive basis and during 4 years I developed following projects: • Constructed and produced AVV, adeno- and retroviral particles for HO-1 transgene expression. • Established several mammalian cell based models through viral infection. • Optimized protocol for sinusoidal endothelial cells isolation from mouse liver. Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University. I was accepted as a lab member on a competitive basis and during 4 years I developed following projects: • Constructed and produced AVV, adeno- and retroviral particles for HO-1 transgene expression. • Established several mammalian cell based models through viral infection. • Optimized protocol for sinusoidal endothelial cells isolation from mouse liver.

Erasmus Research Fellow

• Mar 2010 - Jul 2010

Research internship in A.I. Virtanen Institute funded by Socrates-Erasmus programme. Project: Oncolytic properties of IL-4 enhanced Semliki Forest viral vectors. Research internship in A.I. Virtanen Institute funded by Socrates-Erasmus programme. Project: Oncolytic properties of IL-4 enhanced Semliki Forest viral vectors.

Summer Research Student

• Sep 2009 - Oct 2009

Research internship in Department of Genetic Control of Organ Development and Regeneration funded by Cicerone programme. Project: Characterization of norrin expression pattern at different stages of chick embryo development by in situ hibridization. Research internship in Department of Genetic Control of Organ Development and Regeneration funded by Cicerone programme. Project: Characterization of norrin expression pattern at different stages of chick embryo development by in situ hibridization.