Experience

Azenta Life Sciences

• United States
• Biotechnology Research
• 700 & Above Employee

• Shift Lead

  • Jan 2023 - Present

• Scientist 1

  • Sep 2021 - Jan 2023



ACGT

• United States
• Biotechnology Research
• 1 - 100 Employee

• Molecular Biologist

  • Feb 2020 - Sep 2021

  • Manage and execute multiple GLP, GMP, CAP, GLP Validation, Legal, Enhanced, non-regulatory sequencing projects and ensure their timely completion. • Perform laboratory procedures following SOPs under GLP, GMP and clinical regulations for DNA & RNA extraction, plasmid purification, Gel electrophoresis, PCR primer design, PCR, qPCR, DNA & RNA library construction, Next Generation sequencing, Sanger sequencing and Sanger Data analysis. • Optimize and troubleshoot experimental workflows and… Show more • Manage and execute multiple GLP, GMP, CAP, GLP Validation, Legal, Enhanced, non-regulatory sequencing projects and ensure their timely completion. • Perform laboratory procedures following SOPs under GLP, GMP and clinical regulations for DNA & RNA extraction, plasmid purification, Gel electrophoresis, PCR primer design, PCR, qPCR, DNA & RNA library construction, Next Generation sequencing, Sanger sequencing and Sanger Data analysis. • Optimize and troubleshoot experimental workflows and equipment. • Analyze DNA sequences and chromatograms for reactions and tabulate results as per regulations. • Maintaining accurate laboratory records and complete electronic laboratory notebooks. • Independently analyze and summarize results for internal review and written technical reports. • Conduct Operation Qualification/Performance Qualification of laboratory equipments using SOPs in compliance with GLP/GMP guidelines by US FDA and EPA, CAP regulations. • Responsible for assigned lab duties, maintaining lab stocks, solutions, reagents, supplies and sample/reagent inventory. Show less • Manage and execute multiple GLP, GMP, CAP, GLP Validation, Legal, Enhanced, non-regulatory sequencing projects and ensure their timely completion. • Perform laboratory procedures following SOPs under GLP, GMP and clinical regulations for DNA & RNA extraction, plasmid purification, Gel electrophoresis, PCR primer design, PCR, qPCR, DNA & RNA library construction, Next Generation sequencing, Sanger sequencing and Sanger Data analysis. • Optimize and troubleshoot experimental workflows and… Show more • Manage and execute multiple GLP, GMP, CAP, GLP Validation, Legal, Enhanced, non-regulatory sequencing projects and ensure their timely completion. • Perform laboratory procedures following SOPs under GLP, GMP and clinical regulations for DNA & RNA extraction, plasmid purification, Gel electrophoresis, PCR primer design, PCR, qPCR, DNA & RNA library construction, Next Generation sequencing, Sanger sequencing and Sanger Data analysis. • Optimize and troubleshoot experimental workflows and equipment. • Analyze DNA sequences and chromatograms for reactions and tabulate results as per regulations. • Maintaining accurate laboratory records and complete electronic laboratory notebooks. • Independently analyze and summarize results for internal review and written technical reports. • Conduct Operation Qualification/Performance Qualification of laboratory equipments using SOPs in compliance with GLP/GMP guidelines by US FDA and EPA, CAP regulations. • Responsible for assigned lab duties, maintaining lab stocks, solutions, reagents, supplies and sample/reagent inventory. Show less



University of Cincinnati

• United States
• Higher Education
• 700 & Above Employee

• Microbiology/ Molecular biology Laboratory Technician

  • Aug 2019 - Feb 2020

• Graduate Teaching Assistant

  • Aug 2016 - Feb 2020

  -Have been awarded as an outstanding teaching assistant for the upper-level class in 2018. -Microbiology teaching assistant under Dr.Richard Karp for the courses of 4011 (microbiology for majors) and 2188 (microbiology for nursing). - My role as a teaching assistant involved but was not limited to demonstrating and assisting students in carrying out basic microbiology techniques. I was also responsible for designing and grading quizzes and grading weekly lab reports. - Techniques… Show more -Have been awarded as an outstanding teaching assistant for the upper-level class in 2018. -Microbiology teaching assistant under Dr.Richard Karp for the courses of 4011 (microbiology for majors) and 2188 (microbiology for nursing). - My role as a teaching assistant involved but was not limited to demonstrating and assisting students in carrying out basic microbiology techniques. I was also responsible for designing and grading quizzes and grading weekly lab reports. - Techniques include: >Autoclave, Laminar flow cabinet and incubators >Basic microbiology techniques: Media making, pour and spread plates and streaking for isolation. >Aseptic handling of cultures and media. >Growth curve, serial dilution, and plate counts. >Culturing aerobic and anaerobic bacteria (Gas Pak Jars) >Phage titer and synthetic epidemic >Staining techniques: Gram stain, Acid-fast stain, and Endospore stain. >Using Compound and Phase contrast microscope. >Setting and observing Winogradsky columns. >Minimum Inhibitory Concentration(MIC) and testing antibiotic resistance using Kirby-Bauer test (Disk diffusion). >Environmental sampling: Isolating and identifying an unknown environmental bacteria using a combination of microscopic and biochemical tests following Bergey's Manual.

• Graduate Research Assistant

  • Aug 2015 - Jul 2016

  -Completed analysis of the wood and seed samples from an Archaeological site called Nagaur located in India. A full report on which is available in the Lentz lab. -Co-author on a manuscript where I contributed to Statistical analysis and Data interpretation on a paper authored by Dr. Lentz. Attached below is the link to the research paper: http://www.sciencedirect.com/science/article/pii/S2352409X16302863



EXPORT INSPECTION AGENCY, LABORATORY-PILOT TEST HOUSE

• Mumbai, India

• Quality Control Analyst

  • Jul 2013 - Feb 2015

  As a quality control analyst, I carried out microbial and chemical analysis of food samples and ensured their confirmation to the safety standards laid down in the Food Safety and Standards Authority of India (FSSAI) which operates on similar lines as FDA. I had the following duties as a Quality control Analyst: -Carrying out quality tests on food samples as per the requirements and SOP's of FSSAI and following the protocols of Good Laboratory Practices. -Completing quality reports and… Show more As a quality control analyst, I carried out microbial and chemical analysis of food samples and ensured their confirmation to the safety standards laid down in the Food Safety and Standards Authority of India (FSSAI) which operates on similar lines as FDA. I had the following duties as a Quality control Analyst: -Carrying out quality tests on food samples as per the requirements and SOP's of FSSAI and following the protocols of Good Laboratory Practices. -Completing quality reports and confirming their compliance with the necessary quality standards. -Reporting samples found non-complaint to the quality standards and generating follow up reports. -Training team members on the procedures of food testing and the importance of GLP. Instruments handled: pH meter, quantifying pesticides in fruits using Gas chromatography, UV spectrophotometry, aflatoxin analysis using solid-phase extraction cartridges, protein content, alcohol content using distillation etc. Microbiology techniques: Total and Aerobic plate count, yeast and mold, coliforms, testing for specific bacteria E.coli, S. aureus, B. cereus, L. monocytogenes, Salmonella spp., Vibrio spp., Shigella spp., etc. as per the protocols of USFDA-BAM. Show less As a quality control analyst, I carried out microbial and chemical analysis of food samples and ensured their confirmation to the safety standards laid down in the Food Safety and Standards Authority of India (FSSAI) which operates on similar lines as FDA. I had the following duties as a Quality control Analyst: -Carrying out quality tests on food samples as per the requirements and SOP's of FSSAI and following the protocols of Good Laboratory Practices. -Completing quality reports and… Show more As a quality control analyst, I carried out microbial and chemical analysis of food samples and ensured their confirmation to the safety standards laid down in the Food Safety and Standards Authority of India (FSSAI) which operates on similar lines as FDA. I had the following duties as a Quality control Analyst: -Carrying out quality tests on food samples as per the requirements and SOP's of FSSAI and following the protocols of Good Laboratory Practices. -Completing quality reports and confirming their compliance with the necessary quality standards. -Reporting samples found non-complaint to the quality standards and generating follow up reports. -Training team members on the procedures of food testing and the importance of GLP. Instruments handled: pH meter, quantifying pesticides in fruits using Gas chromatography, UV spectrophotometry, aflatoxin analysis using solid-phase extraction cartridges, protein content, alcohol content using distillation etc. Microbiology techniques: Total and Aerobic plate count, yeast and mold, coliforms, testing for specific bacteria E.coli, S. aureus, B. cereus, L. monocytogenes, Salmonella spp., Vibrio spp., Shigella spp., etc. as per the protocols of USFDA-BAM. Show less



Ipca Laboratories Limited

• India
• Pharmaceutical Manufacturing
• 700 & Above Employee

• Project Intern

  • May 2012 - Jul 2012

  Intern in the Biotechnology (R & D) department at IPCA Laboratories, Mumbai under Dr. Umesh Luthra. >Part of a team working towards optimizing the production of a confidential biological molecule from a fungal strain, using a pilot-scale fermenter. >Monitored the bioreactor with respect to its dissolved oxygen, pH and temperature readings. >Collected broth samples at predetermined intervals from the bioreactor and measured the glucose, nitrogen, and sulfur concentration using… Show more Intern in the Biotechnology (R & D) department at IPCA Laboratories, Mumbai under Dr. Umesh Luthra. >Part of a team working towards optimizing the production of a confidential biological molecule from a fungal strain, using a pilot-scale fermenter. >Monitored the bioreactor with respect to its dissolved oxygen, pH and temperature readings. >Collected broth samples at predetermined intervals from the bioreactor and measured the glucose, nitrogen, and sulfur concentration using UV spectrophotometric techniques. >Purified broth samples as per SOP and analyzed using High-Performance Liquid Chromatography. Show less Intern in the Biotechnology (R & D) department at IPCA Laboratories, Mumbai under Dr. Umesh Luthra. >Part of a team working towards optimizing the production of a confidential biological molecule from a fungal strain, using a pilot-scale fermenter. >Monitored the bioreactor with respect to its dissolved oxygen, pH and temperature readings. >Collected broth samples at predetermined intervals from the bioreactor and measured the glucose, nitrogen, and sulfur concentration using… Show more Intern in the Biotechnology (R & D) department at IPCA Laboratories, Mumbai under Dr. Umesh Luthra. >Part of a team working towards optimizing the production of a confidential biological molecule from a fungal strain, using a pilot-scale fermenter. >Monitored the bioreactor with respect to its dissolved oxygen, pH and temperature readings. >Collected broth samples at predetermined intervals from the bioreactor and measured the glucose, nitrogen, and sulfur concentration using UV spectrophotometric techniques. >Purified broth samples as per SOP and analyzed using High-Performance Liquid Chromatography. Show less



Mithibai College

• Education Administration Programs
• 1 - 100 Employee

• Summer Internship

  • May 2010 - Jun 2010

  The internship was held by the Microbiology dept. at Mithibai College, Mumbai, and completed under the guidance of Dr. Dileep Kamat. It aided in the understanding of the importance of identifying antibiotic producing microbes, isolation and culturing techniques, industrial scale production and downstream purification processes. The process and stages of animal and human clinical trials for drug approval. The internship was held by the Microbiology dept. at Mithibai College, Mumbai, and completed under the guidance of Dr. Dileep Kamat. It aided in the understanding of the importance of identifying antibiotic producing microbes, isolation and culturing techniques, industrial scale production and downstream purification processes. The process and stages of animal and human clinical trials for drug approval.