Jade Stair
Research Technician at Seattle Institute for Biomedical and Clinical Research- Claim this Profile
Click to upgrade to our gold package
for the full feature experience.
Topline Score
Bio
Experience
-
Seattle Institute for Biomedical and Clinical Research
-
United States
-
Research Services
-
1 - 100 Employee
-
Research Technician
-
Mar 2020 - Present
-
-
-
Western Washington University
-
United States
-
Higher Education
-
700 & Above Employee
-
Undergraduate Researcher
-
Mar 2019 - Dec 2019
Advisor: Dr. Lina Dahlberg* Wrote and received full funding for a Research and Sponsored Programs grant provided by Western Washington University* Investigated the role of the ERAD pathway on the regulation of the glutamate receptor GLR-1 in C. elegans* Performed western blotting to compare GLR-1::GFP protein abundance between different ERAD mutant strains * Used the SapTrap method for designing CRISPR-Cas reagents; designed sgRNA and homology arm constructs for recombination around the marc-6 gene locus* Collaborated with other lab members to troubleshoot experiments and maintain stock solutions and reagents
-
-
Cell Biology Lab
-
Mar 2019 - Jun 2019
Authentic research-based course (BIOL 484) focusing on quantitative microscopy* Gained knowledge and experience in Koehler illumination, brightfield, phase contrast, and fluorescence microscopy as well as deconvolution* Compared wild type and Rsp1Δ Tetrahymena thermophila ciliary phenotypes* Investigated the effects of cytochalasin D on actin and primary cilia formation in mouse fibroblasts (NIH 3T3 cells)* Prepared figures of results in Inkscape
-
-
Advanced Cellular and Molecular Biology Lab
-
Jan 2019 - Mar 2019
Authentic research-based course (BIOL 487) focusing on ERAD and neuronal protein regulation* Investigated the effects of ERAD machinery mutations on the regulation of the glutamate receptor GLR-1 in C. elegans* Engineered a transgenic C. elegans strain and confirmed its genotype using PCR* Collaborated with another research lab to examine the role of NMDA and AMPA-type glutamate receptors on learning and memory * Gained experience with western blotting, RNA isolation, and cDNA synthesis* Prepared and presented a final poster as well as a µPub write up of results
-
-
-
-
Janitor
-
Aug 2018 - Aug 2019
* Cleaned, vacuumed, and dusted areas of commercial buildings to maintain an organized and professional environment for clients* Sanitized and scrubbed restroom fixtures including toilets, counters, sinks, and mirrors* Emptied trash, recycling, and restocked restroom supplies as needed * Cleaned, vacuumed, and dusted areas of commercial buildings to maintain an organized and professional environment for clients* Sanitized and scrubbed restroom fixtures including toilets, counters, sinks, and mirrors* Emptied trash, recycling, and restocked restroom supplies as needed
-
-
-
-
Sales Associate
-
Jun 2017 - Sep 2017
* Watered and cared for outdoor plants* Arranged plant displays to maintain visual appeal* Assisted customers in locating and transporting items * Watered and cared for outdoor plants* Arranged plant displays to maintain visual appeal* Assisted customers in locating and transporting items
-
-
Education
-
Western Washington University
Bachelor of Science - BS, Cell/Cellular and Molecular Biology