Experience

Netherlands Forensic Institute NFI

• Netherlands
• Research
• 400 - 500 Employee

• Onderzoeksmedewerker

  • Jul 2022 - Present



Erasmus MC

• Netherlands
• Hospitals and Health Care
• 700 & Above Employee

• Research Technician Molecular Diagnostics - Pathology

  • Jul 2011 - Jun 2022

  Molecular profiling of FFPE tumor tissue samples to determine the molecular characteristics of the tumor. Personalized medicine is often based on specific mutations found in these tumors. These therapies are often used for the treatment of lung, colon, breast and ovarian cancer. The lab also investigates whether multiple tumors are clonally related, we perform Lynch diagnostics and assist in the molecular grading of neurological cancers. To keep up with new developments in the medical world, new assays are frequently tested and implemented. Overview of techniques most used by me: * Next- generation sequencing (IonTorrent platform) e.g. Mutation and Copy Number analysis, Tumor Mutational Burden (TMB) analysis. * RNA sequencing (ArcherDx) e.g. gene fusion analysis * Lynch syndrome diagnostics e.g. IHC, MSI analysis, promotor methylation (MS-MLPA) * Bisulfite conversion based assays e.g. methylationarray, methylation specific PCR. Show less



Leiden University Medical Center

• Netherlands
• Hospitals and Health Care
• 700 & Above Employee

• Intern Department of experimental Haematology

  • Nov 2010 - Jun 2011

  Identifying new T-cell target epitopes and the first steps to an In Vitro model of invasive Aspergillosis. Techniques: Cell culture, ELISA, Fluorscent- Activated Cell Sorting (FACS) and Magnetic- Activated Cell sorting (MACS) Identifying new T-cell target epitopes and the first steps to an In Vitro model of invasive Aspergillosis. Techniques: Cell culture, ELISA, Fluorscent- Activated Cell Sorting (FACS) and Magnetic- Activated Cell sorting (MACS)



Erasmus MC

• Netherlands
• Hospitals and Health Care
• 700 & Above Employee

• Intern Department of Pathology

  • Feb 2010 - Jul 2010

  Improving Lynch syndrome diagnostics. Development of an SNP based assay to determine LOH status of the MLH1 gene. Techniques: multiplex SNaPshot analysis and Sanger sequencing Improving Lynch syndrome diagnostics. Development of an SNP based assay to determine LOH status of the MLH1 gene. Techniques: multiplex SNaPshot analysis and Sanger sequencing



Erasmus MC

• Netherlands
• Hospitals and Health Care
• 700 & Above Employee

• Intern Department of Paediatric Oncology/Heamatology

  • Sep 2008 - Jun 2009

  L-Asparaginase resistance in infant MML- rearranged acute lymphatic leukemia and the involvement of the sphingolipid metabolism. Techniques: Western Blotting, Real- Time PCR, Reverse Transcriptase PCR, Cell culture, Cytotoxicity assay (MTT). L-Asparaginase resistance in infant MML- rearranged acute lymphatic leukemia and the involvement of the sphingolipid metabolism. Techniques: Western Blotting, Real- Time PCR, Reverse Transcriptase PCR, Cell culture, Cytotoxicity assay (MTT).



Maastricht University

• Netherlands
• Higher Education
• 700 & Above Employee

• Intern Department of surgery

  • Sep 2007 - Mar 2008

  TLR4/MD2 involvement in gut immunity. Techniques used: Immunohistochemistry, conventional PCR, Cell culture and Cloning TLR4/MD2 involvement in gut immunity. Techniques used: Immunohistochemistry, conventional PCR, Cell culture and Cloning