Experience

Viroclinics-DDL

• Netherlands
• Biotechnology Research
• 200 - 300 Employee

• Technician BSL3

  • Oct 2020 - Present



EVBox

• Netherlands
• Appliances, Electrical, and Electronics Manufacturing
• 500 - 600 Employee

• Customer Support Representative

  • Jan 2020 - Apr 2020



ProQR Therapeutics

• Netherlands
• Biotechnology Research
• 100 - 200 Employee

• Researcher

  • Feb 2016 - Jan 2020

  Various techniques all for the detection of RNA based medicins and its effects. iPSC culture (incl. differentiation) Cell culture Transfection RNA extraction (real-time) PCR Digital Droplet PCR Western Blot Immunochemistry (cyto + histo) In Situ Hybridization (confocal) Microscopy Various techniques all for the detection of RNA based medicins and its effects. iPSC culture (incl. differentiation) Cell culture Transfection RNA extraction (real-time) PCR Digital Droplet PCR Western Blot Immunochemistry (cyto + histo) In Situ Hybridization (confocal) Microscopy



BPRC

• Netherlands
• Research
• 1 - 100 Employee

• Research Technician

  • Apr 2013 - Feb 2016

  Various PCR for viral detection and PCR assay development Sequencing Western blot for the detection of antibodies of respiratory viruses in serum Magnetic bead assay development on Luminex Viral load assays with real-time PCR DNA/RNA isolation of various samples (tissues, swabs, blood i.a.) Antibody testing with ELISA Cloning Hepatocyte isolation for Malaria research Mosquito dissection Various PCR for viral detection and PCR assay development Sequencing Western blot for the detection of antibodies of respiratory viruses in serum Magnetic bead assay development on Luminex Viral load assays with real-time PCR DNA/RNA isolation of various samples (tissues, swabs, blood i.a.) Antibody testing with ELISA Cloning Hepatocyte isolation for Malaria research Mosquito dissection



Erasmus MC

• Netherlands
• Hospitals and Health Care
• 700 & Above Employee

• Research Technician / Intern

  • Nov 2011 - Apr 2013

  Identification of genes involved in the genesis and progression of esophageal adenocarcinoom. Mainly looking at genes that are over expressed Data analysis using Partek and CNAG 2.0. Primer design, PCR, sequencing, fragment analysis, reverse transcriptase PCR and immunohistochemistry MPLA and qPCR (Taqman Assays) to indicate the amplification on DNA level Supervise and guide fourth year intern Creation of a database with Microsoft Access to keep track of the research that is done on esophageal cancer in the department Show less



PathoFinder B.V.

• Netherlands
• Medical Equipment Manufacturing
• 1 - 100 Employee

• Intern

  • Sep 2010 - Jan 2011

  Development of a multiplex kit for the detection of pathogens by means of PCR and MLPA Primer/ probe design, PCR, Real-time PCR, fragment analyisis Development of a multiplex kit for the detection of pathogens by means of PCR and MLPA Primer/ probe design, PCR, Real-time PCR, fragment analyisis