Helle Knudsen
Laborant at Gubra- Claim this Profile
Click to upgrade to our gold package
for the full feature experience.
-
English Full professional proficiency
-
Swedish Elementary proficiency
-
German Limited working proficiency
Topline Score
Bio
Camilla Thormod Hjort
Jeg har arbejdet sammen med Helle på Santaris pharma gennem 5 år og giver hende mine varmeste anbefalinger. Helle er en skøn pige med masser af humør. Hun er en super dygtig laborant der for ting til at ske.
Emiliano Cló
I was the Senior Scientist associated with Helle throughout her employment at Novo Nordisk. We worked together within the Research Chemistry VP area. Helle proved her worth in the lab every day by consistently providing results of sound quality. She was engaged in an array of diversified project tasks spanning from insulin modification to peptide synthesis to site-selective protein modification. She has very good practical skills in the lab and she is able to manage several parallel project activities at once. Moreover, she is an experienced used of HPLC/UPLC systems (both analytical and preparative) and was superuser of Waters UPLC systems. She is well acquainted with protein purification and the use of GE Åkta FPLC systems (SEC, IXC, affinity). She has a very positive attitude to work and social situations alike which makes her a valuable team player .
Camilla Thormod Hjort
Jeg har arbejdet sammen med Helle på Santaris pharma gennem 5 år og giver hende mine varmeste anbefalinger. Helle er en skøn pige med masser af humør. Hun er en super dygtig laborant der for ting til at ske.
Emiliano Cló
I was the Senior Scientist associated with Helle throughout her employment at Novo Nordisk. We worked together within the Research Chemistry VP area. Helle proved her worth in the lab every day by consistently providing results of sound quality. She was engaged in an array of diversified project tasks spanning from insulin modification to peptide synthesis to site-selective protein modification. She has very good practical skills in the lab and she is able to manage several parallel project activities at once. Moreover, she is an experienced used of HPLC/UPLC systems (both analytical and preparative) and was superuser of Waters UPLC systems. She is well acquainted with protein purification and the use of GE Åkta FPLC systems (SEC, IXC, affinity). She has a very positive attitude to work and social situations alike which makes her a valuable team player .
Camilla Thormod Hjort
Jeg har arbejdet sammen med Helle på Santaris pharma gennem 5 år og giver hende mine varmeste anbefalinger. Helle er en skøn pige med masser af humør. Hun er en super dygtig laborant der for ting til at ske.
Emiliano Cló
I was the Senior Scientist associated with Helle throughout her employment at Novo Nordisk. We worked together within the Research Chemistry VP area. Helle proved her worth in the lab every day by consistently providing results of sound quality. She was engaged in an array of diversified project tasks spanning from insulin modification to peptide synthesis to site-selective protein modification. She has very good practical skills in the lab and she is able to manage several parallel project activities at once. Moreover, she is an experienced used of HPLC/UPLC systems (both analytical and preparative) and was superuser of Waters UPLC systems. She is well acquainted with protein purification and the use of GE Åkta FPLC systems (SEC, IXC, affinity). She has a very positive attitude to work and social situations alike which makes her a valuable team player .
Camilla Thormod Hjort
Jeg har arbejdet sammen med Helle på Santaris pharma gennem 5 år og giver hende mine varmeste anbefalinger. Helle er en skøn pige med masser af humør. Hun er en super dygtig laborant der for ting til at ske.
Emiliano Cló
I was the Senior Scientist associated with Helle throughout her employment at Novo Nordisk. We worked together within the Research Chemistry VP area. Helle proved her worth in the lab every day by consistently providing results of sound quality. She was engaged in an array of diversified project tasks spanning from insulin modification to peptide synthesis to site-selective protein modification. She has very good practical skills in the lab and she is able to manage several parallel project activities at once. Moreover, she is an experienced used of HPLC/UPLC systems (both analytical and preparative) and was superuser of Waters UPLC systems. She is well acquainted with protein purification and the use of GE Åkta FPLC systems (SEC, IXC, affinity). She has a very positive attitude to work and social situations alike which makes her a valuable team player .
Experience
-
Gubra
-
Denmark
-
Biotechnology Research
-
100 - 200 Employee
-
Laborant
-
Jan 2022 - Present
-
-
-
CytoVac AB
-
Biotechnology Research
-
1 - 100 Employee
-
Laboratory Technician - QC & R&D
-
Jan 2019 - Dec 2021
Responsible for in-house Quality Control Analysis Development, validation and performance of in-house Quality Control Analysis (FACS, Endotoxin, In vitro Potency assay) Preparation of Working Cell Bank Gene expression analysis - qPCR Performing and reviewing of GMP documentation and reports Training of laboratory personnel according to GMP Data processing and reporting Responsible for all purchases in the organization including contact to suppliers Responsible for in-house Quality Control Analysis Development, validation and performance of in-house Quality Control Analysis (FACS, Endotoxin, In vitro Potency assay) Preparation of Working Cell Bank Gene expression analysis - qPCR Performing and reviewing of GMP documentation and reports Training of laboratory personnel according to GMP Data processing and reporting Responsible for all purchases in the organization including contact to suppliers
-
-
-
Xcelcyte A/S
-
Hoersholm, Denmark
-
Laboratory Technician - QC, R&D and Supply Chain
-
Jul 2020 - Jul 2021
(Xcelcyte A/S rented offices and laboratory facilities at CytoVac A/S. As a part of this agreement, CytoVac A/S employees were hired to work part time in Xcelcyte A/S) Responsible for establishment of ordering system for laboratory supplies Responsible for all purchases in the organization including contact to suppliers and establishment of supplier contracts ELISA analysis of serum cytokines Coupling of antibodies to Dynabeads Data processing, documentation and reporting (Xcelcyte A/S rented offices and laboratory facilities at CytoVac A/S. As a part of this agreement, CytoVac A/S employees were hired to work part time in Xcelcyte A/S) Responsible for establishment of ordering system for laboratory supplies Responsible for all purchases in the organization including contact to suppliers and establishment of supplier contracts ELISA analysis of serum cytokines Coupling of antibodies to Dynabeads Data processing, documentation and reporting
-
-
-
Novo Nordisk
-
Denmark
-
Pharmaceutical Manufacturing
-
700 & Above Employee
-
Laboratory Technician
-
Dec 2015 - Jan 2019
Laboratory Technician, Global Research Technology - Research Chemistry. Peptide and Protein Chemistry : o Manual and automated solid –phase peptide synthesis o Protein bioconjugation with peptides, nucleic acids and small molecules. o Insulin chemistry o Small molecule linker synthesis Purification and characterization : o FPLC; size-exclusion, ion-exchange and affinity chromatography with Äkta Purifier and Avant instruments o HPLC; method development experience on reverse-phase Agilent analytical HPLC, Waters preparative HPLC, Gilson preparative HPLC o UPLC; SuperUser of Waters Acquity o LC-MS; skilled in MS data analysis o SDS Gel electrophoresis; NuPAGE SDS-PAGE gel system • Extensive data processing, documentation and reporting. Member of the UPLC SuperUser Team : Responsible for maintenance of UPLC equipment, methods and protocols within the department and in teamwork across the function. Member of the MilliQ water systems SuperUser Team : Responsible for maintenance of MilliQ systems Show less
-
-
-
Bavarian Nordic
-
Denmark
-
Pharmaceutical Manufacturing
-
700 & Above Employee
-
Laboratory Technician
-
Feb 2015 - Nov 2015
Laboratory Technician in QC - Chemistry and VirusTitration. QC Chemistry : Chemical analysis of raw materials and products. Measurement of pH and Appearance, Residual Moisture (Karl Fischer Titration), Total Protein (ELISA) and Residual Benzonase (ELISA). Performing and reviewing of GMP documentation QC Virus Titration : Titration of poxviruses using ELISA based detection Seeding of CEF-cells, virus titration, staining and data calculation. Performing of GMP documentation Show less
-
-
-
-
Lab tech
-
May 2008 - Nov 2014
Departement of Oligoproduction : formulation of oligoes to in vivo and in vitro studies. • Analytical Chemistry : Oligonucleotidecontent in tissue by HPLC and ELISA • In vitro studies : Cell culture experiments studying mechanism and potency of target-specific anti-sense oligonucleotides (by unassisted uptake and transfection), Isolation of RNA from cells, followed by cDNA synthesis and qPCR • In vivo studies: o Isolation of RNA from different kinds of tissue, followed by cDNA synthesis and qPCR o Analysis of serum proteins and serum lipids by FPLC, tissue lipid extraction, HPTLC and colorimetric assays o Gel electrophoresis and Western blot analysis o Serum cytokine analysis by ELISA, including method validation and optimization of assays for serum from different species • Extensive data processing and reporting • Laboratory coordinator for Metabolic Diseases Show less
-
-
-
Precisense A/S
-
Medical Equipment Manufacturing
-
1 - 100 Employee
-
Lab tech
-
2003 - 2008
Laboratory technician, Organic Synthesis and Protein Chemistry Participated in setting up an organic chemistry laboratory (buying equipment, established collaboration with suppliers etc) • Organic synthesis of dyes and fluorophores • Purification and characterization of organic compounds by HPLC, TLC, NMR and MS • Synthesis and characterization of amino dextrans • Responsible for all the daily routines in the lab, purchase of equipment, consumer goods • and contact to suppliers • Data processing and reporting Participated in offsetting up a Protein Chemistry laboratory (buying equipment, established collaboration with suppliers etc) Development and optimization of ELISA assays Purification and staining of proteins and amino dextrans Dialysis Main focus on Purification of proteins and dextans by chromatographic principles at ÄktaPrime equipment (ion exchange and gel filtration). Method development, optimization and maintenance of columns and equipment Data processing and reporting Appointed safety representative in 2005 and participated in establishment of a security organization and preparation of a workplace risk assessment “APV” Show less
-
-
-
Lundbeck
-
Denmark
-
Pharmaceutical Manufacturing
-
700 & Above Employee
-
Lab tech
-
2001 - 2003
Laboratory technician in department of Combinatorial Chemistry • Solid Phase- and parallel synthesis of compound libraries on different kinds of automatized equipment • Combinatorial Chemistry • Organic synthesis, purification and characterization by TLC, NMR and MS • Processing of data in internal data systems Laboratory technician in department of Combinatorial Chemistry • Solid Phase- and parallel synthesis of compound libraries on different kinds of automatized equipment • Combinatorial Chemistry • Organic synthesis, purification and characterization by TLC, NMR and MS • Processing of data in internal data systems
-
-
-
LEO Pharma
-
Denmark
-
Pharmaceutical Manufacturing
-
700 & Above Employee
-
Lab tech
-
1998 - 2001
Laboratory technician at Department of Medicinal Chemistry. • Organic synthesis • Semi automatic parallel synthesis • Purification methods (HPLC, LC, extraction methods etc.) • Characterization of synthesis products by TLC, NMR and MS. Laboratory technician at Department of Medicinal Chemistry. • Organic synthesis • Semi automatic parallel synthesis • Purification methods (HPLC, LC, extraction methods etc.) • Characterization of synthesis products by TLC, NMR and MS.
-
-
-
-
Lab tech
-
1997 - 1997
Laboratory Technician, Maternity cover. • Spectrophotometric analysis of soil, water and other environmental samples • Sample preparation • PData processing GMP/GLP • Any other jobs around the laboratory Laboratory Technician, Maternity cover. • Spectrophotometric analysis of soil, water and other environmental samples • Sample preparation • PData processing GMP/GLP • Any other jobs around the laboratory
-
-
Education
-
Laborantskolen i Hovedstadsområdet
Lab tech, Chemistry