Experience

Hanyang University

• South Korea
• Higher Education
• 400 - 500 Employee

• Associate Professor

  • Apr 2022 - Present

• Research Associate Professor

  • Sep 2020 - Apr 2022

  Mass cytometry applications based on antibody conjugated biomarkers Profiling of the heterogeneous immune cells in human peripheral blood mononuclear cells  Profiling drug efficacy performance in human peripheral blood mononuclear cells Profiling double-heterogeneities of Oncology- all types of Cancers, Liver diseases- NAFLD, Infectious diseases – Sepsis, Multiple myeloma etc., Drug dose-response relationships at single-cell level Toxicity of nanoparticles or nano-drug interacting with human immune cells sc-RNAseq analysis – 10x chromium analysis Identification of cellular transcriptomic and proteins expression at single cell level Cell type identification based on gene expression of single cell Differently expressed genes (DEG) analysis at single cell level Interpretation of gene regulatory networks and molecular pathways across the cells Good knowledge and hands-on experience in various software including, FlowSOM, Cytobank, 10X chromium Loupe, Network analysis, Molecular pathway analysis (Kegg pathway finder, Reactome pathway finder), Venn diagram and other basic office applications. Identified various chemical, biological, and nano-based drugs for various cancers using in vitro/in vivo studies Show less



Kyung Hee University

• South Korea
• Higher Education
• 700 & Above Employee

• Research Assistant Professor

  • Apr 2018 - Sep 2020

   Identification, characterization and evaluation of various chemical, biological, and nano-based drugs for various cancers/ inflammation using in vitro/in vivo studies Characterization of chemical, biological, and green synthesized nanoparticles  Thin layer chromatography  Column chromatography  FT-IR, HPLC, LC-MS  GC, GC-MS, EI-MS  NMR, 2D-NMR (DEPT, COSY, HMBC, HSQC)  NTA  XRD  FE-TEM  FE-SEM Anti-proliferation assay for various types of cancer  MTT assay  Live/Dead cell detection Metastasis analysis  Colony forming assay  Wound healing assay Pharmacognosy effect  Toxicity assessment of various drugs using Wistar albino rat model Inflammatory pathways using in vitro/in vivo studies  ROS detection  Antioxidant  Autophagy pathway  mTOR/PI3K/AKT mediated pathway  p53 related pathway  Cytokines mediated pathway  MAPK/ERK signaling pathway  JAK/STAT signaling pathway Anti-cancer related molecular pathway analysis  Apoptosis mediated pathway  Autophagy pathway  p53 related pathway  Fatty acid metabolism  mTOR/PI3K/AKT mediated pathway Show less



Seoul National University

• Higher Education
• 700 & Above Employee

• Research Assistant Professor

  • Sep 2012 - Dec 2017

  Anti-proliferation  MTT assay  Live/Dead cell detection Metastasis  Colony forming assay  Wound healing assay Fluorescence staining techniques to detect cell death mechanism  Hoechst staining analysis  Propidium (PI) staining analysis  ROS staining analysis  Mito and Lyso tracker staining analysis Cell imaging analysis to detect live and death cells  Bright-field microscopy  Confocal fluorescence microscopy  Scanning Electron Microscopy (SEM)  Transmission Electron Microscopy (TEM) Molecular biology experiments  DNA fragmentation assay  mRNA gene expression-PCR, RT-PCR, qRT-PCR  Protein expression- SDS-PAGE, Western-Blot, ELISA Cell sorting techniques  Cell cycle- FACS  Cell death detection-FACS II aria Isolation of bioactive compounds  Medicinal plants  Plant essential oils  Edible Insects Characterization techniques  Thin layer chromatography  Column chromatography  FT-IR, HPLC, LC-MS  GC, GC-MS, EI-MS  NMR, 2D-NMR (DEPT, COSY, HMBC, HSQC) Show less