Hanna Zielinska
Bioprocess Scientist 2 and Biological Safety Officer at eXmoor Pharma Concepts Ltd- Claim this Profile
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Polish Native or bilingual proficiency
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English Full professional proficiency
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Bio
Experience
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eXmoor Pharma
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United Kingdom
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Biotechnology Research
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1 - 100 Employee
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Bioprocess Scientist 2 and Biological Safety Officer
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Apr 2021 - Present
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Bioprocess Scientist 1 and Biological Safety Officer
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Sep 2019 - Mar 2021
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University of Bristol
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United Kingdom
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Higher Education
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700 & Above Employee
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Research Technician
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Jan 2018 - Aug 2019
Project: Prostate Cancer- Exercise and Metformin trial. Main duties included: •Collection and handling of and extraction of DNA/RNA/protein from blood and tumour biopsies. •Peripheral blood mononuclear cells (PBMCs) isolation from blood. •Tissue processing and microtomy. •Immunohistochemistry. •Tissue scoring. •Student supervision. Project: Prostate Cancer- Exercise and Metformin trial. Main duties included: •Collection and handling of and extraction of DNA/RNA/protein from blood and tumour biopsies. •Peripheral blood mononuclear cells (PBMCs) isolation from blood. •Tissue processing and microtomy. •Immunohistochemistry. •Tissue scoring. •Student supervision.
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Cabot Learning Federation
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United Kingdom
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Education Administration Programs
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100 - 200 Employee
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Science Technician at Digitech Studio School
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Jan 2017 - Jan 2018
Main duties included: •Preparing practical classes and supporting the teacher throughout the practicals. •Advising teachers and pupils on H&S aspects of their classroom experiments. •Assisting with maintaining standards of H&S in the science department in line with CLEAPSS and HSE recommendations. •Responsibility for risk assessments and COSHH forms and carrying out safety checks. Main duties included: •Preparing practical classes and supporting the teacher throughout the practicals. •Advising teachers and pupils on H&S aspects of their classroom experiments. •Assisting with maintaining standards of H&S in the science department in line with CLEAPSS and HSE recommendations. •Responsibility for risk assessments and COSHH forms and carrying out safety checks.
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University of Bristol
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United Kingdom
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Higher Education
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700 & Above Employee
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PhD Student
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Sep 2013 - Dec 2016
Project title: Hyperglycemia and fibronectin: the criminal partnership during breast cancer progression.Faculty prize for Best Doctoral Research Thesis 2017/2018.Practical skills gained:Tissue culture:• Proficient in all essential tissue culture techniques.• Experience in culturing many different types of cell lines.• 3D organoid culture.• Production and use of lentivirus, including making stable shRNA expressing cell lines.• Transfection and transduction techniques.Assay development:Development, optimization and validation of:▪ siRNA knockdown assays.▪ Functional assays including proliferation, migration and invasion.▪ Intracellular signalling assays.▪ Metabolic assays including glucose uptake, lactate and citrate production.▪ ELISA. Proteomics:▪PAGE, western blotting and immunoprecipitation.Other:• Fluorescent and confocal microscopy.• Digital holographic microscopy.• All basic molecular biology skills such as preparation of DNA, restriction digests, PCR and qPCR.• ddPCR• Radioactive and non-radioactive proliferation and metabolic assays.• Subcellular fractionation.
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Research Technician
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Sep 2012 - Aug 2013
Project title: The role of epithelial splicing regulatory protein 1 (ESRP1) in tumor growth and metastasis.
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Education
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University of Bristol
Doctor of Philosophy - PhD, Molecular Biology -
University of the West of England
Bachelor's degree, Pharmaceutical Sciences