Experience

AGS-TX

• France
• Biotechnology Research
• 1 - 100 Employee

• Lab engineer

  • May 2021 - Present

  Training: - in the preparation of extracellular vesicles (EV) from µalgal culture. - in cloning by module and by complete assembly. - in the transformation of µalgae. - in Endo and Exo loading of µalgae. -in the use of NTA, FACs, TEM, ... Training: - in the preparation of extracellular vesicles (EV) from µalgal culture. - in cloning by module and by complete assembly. - in the transformation of µalgae. - in Endo and Exo loading of µalgae. -in the use of NTA, FACs, TEM, ...



PHARNEXT

• France
• Biotechnology Research
• 1 - 100 Employee

• Research Engineer

  • Apr 2008 - Jan 2021

  Creation of the Pharnext laboratory with a focus on RT-qPCR. - Development of real-time quantitative PCR on Lightcycler 480 Instrument II from cellular and tissue samples (mRNA and miRNA). - Development of more than 160 single or multi-species biomarkers. Animal Experimentation: - Isolation on rat of soleus muscles, sciatic nerves, plasma, brains... References: Together with other in vitro and in vivo assays, allowed to start a phase IIa on a mix of 3 molecules (PXT3003) for CMT1A disease and to successfully complete a phase III biologically. Show less



NAUTILUS BIOTECH

• France
• Biotechnology Research

• Research Assistant

  • Jan 2007 - Mar 2008

  Expertise in biochemistry : - Production and selection of native protein variants, with the objective of improving in vivo stability (extended half-life) or change of administration (oral route; increased resistance to proteolysis in both blood and intestines). -Adaptation of the ELISA serodiagnostic test: changes in range, sensitivity, media used, etc. -Development of new electrophoresis methods and protein analyses (SDS-PAGE), studies of antigen/antibody interactions (Western Blot, ELISA), colorimetric assays. -Work in L2 level laboratory on Cell Biology techniques. Expertise in molecular biology: - Directed mutagenesis and cloning. - Proteolysis to screen and detect the most resistant mutants. Quality assurance : - studies of Repeatability and linearity of immunological techniques for validation. - Drafting and validation of standard protocols (SOPs). Show less



SERONO GENETICS INSTITUTE S.A.

• France
• Environmental Services

• Laboratory manager

  • 2002 - 2006

  Laboratory and team management -Laboratory manager with a team of 7 people. -Implementation of a platform for large-scale cloning of secreted protein genes in expression vectors (RNA extraction, Reverse transcriptase, enzymatic and recombinant cloning, vector modification...). References: More than 1200 different protein genes cloned in several expression vectors. - Several patents: "Erythropoetin polypeptides and uses thereof": (US PTO 60/753,706; European Patent Office 05111265), (USPTO 60/753,668; European Patent Office 05111262.1). BCMA polypeptides and uses thereof " BCMA polypeptides and uses thereof " Patent N° 04293108.9-2401... Show less



Genset Corporation

• United States
• Biotechnology

• Research Engineer

  • 1996 - 2002

  2001-2002 : Research Engineer: cDNA bank screening, bank manager, human genome map curation. 2000-2001 : Project manager : (SNP project) 1996-1999 : Development and production of BACs sub clones. -Interface between the different production teams. Cartography: Curation of large-scale genomic maps. -Sequencing of regions of the human genome (hepatocarcinoma, prostate cancer, schizophrenia...). References : more than 7000 BACs under cloned and more than 20000 new SNPs identified. Show less



CEPH - Fondation Jean Dausset

• Biotechnology
• 1 - 100 Employee

• Senior Research Development Technician

  • 1990 - 1996

  1993-1996: Mapping of the human genome (Ilya CHUMAKOV) under the direction of Daniel COHEN. 1990-1993 : Sequencing of an H.L.A. region on human chromosome 6 (François IRIS). 1993-1996: Mapping of the human genome (Ilya CHUMAKOV) under the direction of Daniel COHEN. 1990-1993 : Sequencing of an H.L.A. region on human chromosome 6 (François IRIS).


Army Hospital Center
• Head of the bacteriology department

  • 1989 - 1990