Experience

Universal Sequencing Technology

• United States
• Biotechnology
• 1 - 100 Employee

• Sr. Scientist

  • Nov 2018 - Present

  Greater San Diego Area



ChemoTactics, Inc

• Chief Scientist & COO

  • Apr 2013 - Nov 2018

  San Diego • Protein production o Designed and generated wild type- and modified chemokine expression constructs. o Developed and optimized protein purification protocol with different chromatographic methods (metal-affinity, ion-exchange, hydrophobic interaction, size exclusion) using gravity column, FPLC, and HPLC. o Optimized chemokine refolding from inclusion bodies. o Maintained mammalian tissue culture for cellular assays. o Maintained bacterial strains for protein… Show more • Protein production o Designed and generated wild type- and modified chemokine expression constructs. o Developed and optimized protein purification protocol with different chromatographic methods (metal-affinity, ion-exchange, hydrophobic interaction, size exclusion) using gravity column, FPLC, and HPLC. o Optimized chemokine refolding from inclusion bodies. o Maintained mammalian tissue culture for cellular assays. o Maintained bacterial strains for protein expression. • Protein characterization o Evaluated the product purity by silver-staining and HPLC. o Validated the molecular weight by MOLDI-TOF mass spectrometry. o Conducted endotonxin assay by LAL (Limulus amebocyte lysate) method. o Conducted biotinylation quantitation assay by HABA-avidin-based method. o Determined the product potency and efficacy by calcium flux and TransWell migration. o Compared the activities of wild type- and modified chemokines. • Product development o Developed and optimized new labeling protocols for biotinylated and fluorescent chemokines. o Participated in the development of applications for the purified reagents in microscopy and flow cytometry. • Database maintenance o Documented the purification steps and assay results for every product. o Created and organized the stock registries for mammalian cell lines and bacterial strains. Show less



Exeligen Scientific, Inc.

• Senior Scientist

  • Jun 2015 - May 2017

  Carlsbad, California • Started up the laboratory operation. • Established and implement the standard operation protocols for manufacturing the generic CRISPR-based genome-editing products. • Developed and optimized the in-vitro and cellular quality control assays. • Engineering a new generation of CRISPR-based genome-editing tool. • Participated in the development of microbiome-based diabetes treatment tools.



UC San Diego

• United States
• Higher Education
• 700 & Above Employee

• Visiting Scholar

  • Apr 2013 - Oct 2013

  La Jolla



UC Santa Barbara

• Higher Education
• 700 & Above Employee

• Research Associate

  • Jan 2012 - May 2012

• Graduate Research Assistant

  • Jul 2005 - Mar 2012

  Advisor: Professor Frederick W. Dahlquist, University of California, Santa Barbara • Designed and generated E.coli chemotaxis protein constructs (CheA P1, CheA P2, CheY and CheW and their mutants) and developed their purification protocols. • Solved a three-protein complex structure (E.coli CheA P1, P2 domains and CheY) using solution NMR techniques. • Studied kinetics of phosphotransfer from P1 domain to CheY using stopped-flow fluorescence technique to elucidate the role of P2 and… Show more Advisor: Professor Frederick W. Dahlquist, University of California, Santa Barbara • Designed and generated E.coli chemotaxis protein constructs (CheA P1, CheA P2, CheY and CheW and their mutants) and developed their purification protocols. • Solved a three-protein complex structure (E.coli CheA P1, P2 domains and CheY) using solution NMR techniques. • Studied kinetics of phosphotransfer from P1 domain to CheY using stopped-flow fluorescence technique to elucidate the role of P2 and the linker between P1 and P2 on the phosphotransfer. • Studied the dynamics of E. coli CheW using NMR and its stability using circular dichroism (CD) to examine the role of a proposed salt bridge. Mentorship: • Trained an intern for collaborative work with the Zhulin laboratory at University of Tennessee

• Teaching Assistant

  • Sep 2004 - Mar 2011

  Lectured, supervised and demonstrated experiments with safety instruction to 24 undergraduates.



University of Oklahoma

• United States
• Higher Education
• 700 & Above Employee

• Undergraduate Research Assistant

  • Jun 2002 - May 2004

  Advisor: Bruce A. Roe, University of Oklahoma, Norman • Involved in mouse and Medicago DNA sequencing projects in The Advanced Center for Genome Technology.