Gowri Poigaialwar
Project Assistant II at Aravind Medical Research Foundation- Claim this Profile
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Bio
Credentials
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Hands-on Data Analysis for Metabolic Profiling
Imperial College LondonNov, 2021- Oct, 2024
Experience
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Aravind Medical Research Foundation
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Research Services
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1 - 100 Employee
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Project Assistant II
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Jul 2018 - Present
Project: Translational Genomics of Paediatric Eye Diseases • Screened the complete mitochondrial genome of LHON patients using Sanger and targeted enrichment; Prediction of risk group by phylogenetic analysis • Developed transmitochondrial cybrid cells as a model system to study LHON by fusing Rho-0 cells with patient platelets • Validated the mitochondrial DNA mutations using the Cybrid cells by functional assays (ATP, ROS and mitochondrial membrane potential measurement) Project: Translational Genomics of Paediatric Eye Diseases • Screened the complete mitochondrial genome of LHON patients using Sanger and targeted enrichment; Prediction of risk group by phylogenetic analysis • Developed transmitochondrial cybrid cells as a model system to study LHON by fusing Rho-0 cells with patient platelets • Validated the mitochondrial DNA mutations using the Cybrid cells by functional assays (ATP, ROS and mitochondrial membrane potential measurement)
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Centre for Stem Cell Research ,Department of Biotechnology,Christian Medical College
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India
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Higher Education
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1 - 100 Employee
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Research Intern
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Dec 2017 - May 2018
Project: CRISPR aided genome editing to study beta Hemoglobinopathies • CRISPR mediated knockdown of BCL11A enhancer region in the human erythroid progenitors • Guide RNA designing and cloning into cas9 expressing vector backbone • Lipofection of plasmids into HEK 293T Cells to produce Lentivirus • Sorting of transfected cells using Fluorescent Activated Cell Sorting (FACS) • Transduction of virus in HUDEP-2 to create mutations in the target sites of BCL11A enhancer • Sorting the transduced cells by FACS for the selection marker • Detecting the indels by performing T7 endonuclease assay Show less
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Jawaharlal Nehru University
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India
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Higher Education
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700 & Above Employee
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Research Intern
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Dec 2015 - May 2016
Project: Study of transcription factors (IRF-1 and IRF-2) in Mouse Bone Marrow Cells • Isolation and culture of mouse bone marrow cells (mBMCs) • Cell cycle analysis of mBMCs by Fluorescent Activated Cell Sorting • Localization of transcription factors IRF-1 and IRF-2 in mBMCs using immunocytochemistry • Measurement of expression of IRF-1 and IRF-2 in BMCs using RT-PCR Project: Study of transcription factors (IRF-1 and IRF-2) in Mouse Bone Marrow Cells • Isolation and culture of mouse bone marrow cells (mBMCs) • Cell cycle analysis of mBMCs by Fluorescent Activated Cell Sorting • Localization of transcription factors IRF-1 and IRF-2 in mBMCs using immunocytochemistry • Measurement of expression of IRF-1 and IRF-2 in BMCs using RT-PCR
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Summer Research Student
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May 2015 - Jun 2015
Project: Identification and Quantification of Human Trabecular Meshwork Stem Cells • Isolation of human Trabecular Meshwork (TM) tissue from the cadaver corneoscleral button • Isolation of TM cells by collagenase treatment and preparation of cytospin smears • Cryosectioning of fresh tissue segments (cornea and limbus) • Hematoxylin and eosin staining of tissue sections, Giemsa staining of cytospin smear • Localization and quantification of TM stem cells (TMSCs) using stem cell markers Show less
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Education
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Bharathidasan University
Master of Technology - MTech, Biotechnology
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