Experience

ExcellGene SA

• Switzerland
• Biotechnology Research
• 1 - 100 Employee

• DSP Engineer

  • Jun 2016 - Present

  -Purification at small and medium scale of recombinant proteins produced in mammalian cells.-Design and implementation of DSP processes using chromatographic methods: Affinity, SEC, IEX, HIC. Equipments: Amersham Biosciences AKTA FPLC, AKTA avant 150, AKTA pilot. Software: Unicorn versions 4 and 7.-Column packing: tricorn, HiScale.-Buffer exchange and concentration using TFF systems. -Purification at small and medium scale of recombinant proteins produced in mammalian cells.-Design and implementation of DSP processes using chromatographic methods: Affinity, SEC, IEX, HIC. Equipments: Amersham Biosciences AKTA FPLC, AKTA avant 150, AKTA pilot. Software: Unicorn versions 4 and 7.-Column packing: tricorn, HiScale.-Buffer exchange and concentration using TFF systems.



ExcellGene SA

• Switzerland
• Biotechnology Research
• 1 - 100 Employee

• Laboratory Assistant

  • Sep 2012 - Oct 2015

  - Molecular biology: Construction, amplification and purification of plasmids, agarose gel electrophoresis, quantification of nucleic acids, usage of cloning softwares- Cell culture: Cells freezing and thawing, cell counting with a hemocytometer, maintenance and amplification of cells in suspension and adherents, stable and transient transfection, culture monitoring by flow cytometry and NOVA- Analytics: ELISA, Polyacrylamide gel electrophoresis - Molecular biology: Construction, amplification and purification of plasmids, agarose gel electrophoresis, quantification of nucleic acids, usage of cloning softwares- Cell culture: Cells freezing and thawing, cell counting with a hemocytometer, maintenance and amplification of cells in suspension and adherents, stable and transient transfection, culture monitoring by flow cytometry and NOVA- Analytics: ELISA, Polyacrylamide gel electrophoresis