Nicholas Garcia

PhD Student at University of Colorado Anschutz Medical Campus
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Contact Information
us****@****om
(386) 825-5501
Location
Denver, Colorado, United States, US

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Experience

    • United States
    • Higher Education
    • 700 & Above Employee
    • PhD Student
      • Aug 2021 - Present

    • United States
    • Hospitals and Health Care
    • 700 & Above Employee
    • Technical Research Assistant II
      • Jul 2019 - May 2021

      • Supported post-doctoral investigators in the Wesemann lab in the Division of Allergy and Clinical Immunology by performing data analysis and visualization, sequence optimization, and other bioinformatic tasks• Analyzed V segment use, somatic hypermutation, and clonal expansion of human and mouse Ig repertoire deep sequencing datasets• Identified isotype-specific differential gene expression in human B cell 10x dataset• Investigated impact of IgA deficiency on gut microflora and virome composition and abundance in datasets generated from human and mouse stool samples• Modeled protein stability of Ig and VLR based antibodies and identified structural features that differentiate VLR and Ig antibodies and limit the potential for VLR somatic hypermutation and affinity maturation in vivo and• Developed R package (unpublished) to facilitate automated analysis of protein stability modeling using Foldx• Cloned and expressed Ig and VLR based antibodies to validate computational stability predictions using fluorescence based stability assay• Carried out basic bench work in support of various lab projects• Promoted from Technical Research Assistant I July 2020

    • United States
    • Higher Education
    • 700 & Above Employee
    • User Services Senior Student Team Lead
      • Aug 2015 - Jun 2019

      • Managed team of students responsible for responding to phone and walk-in IT service requests from faculty, staff, and other students• Provided campus-wide support on a variety of technical issues, including monitor and network setup• Increased number of service requests handled by student team from 5 to 50 percent by growing team from 2 to 15 members• Developed training resources and reference material for the Student Help Desk Team• Assisted with new team member onboarding and orientation, and led training sessions in conjunction with the professional staff• Generated team work schedules and completed bi-weekly time sheets• Promoted from Student Team Member June 2017

    • Union College Research Fellow
      • Jun 2018 - Aug 2018

      • Developed mobile robotics platform to augment the teaching and research capabilities of the Union College Social Robotics Lab• Utilized on-site machining resources and off-the-shelf electronic components to design an inexpensive mobile robot capable of omni-directional movement• Employed the Robot Operating System to enable semi-autonomous navigation, environment mapping, and 3D vision

    • Union College Research Fellow
      • Jun 2017 - Aug 2017

      • Implemented computational pipeline to process and analyze extracellular recordings of dragonfly nerve cords• Utilized MATLAB to convert LabChart output to a format compatible with the spike sorting software Combinato• Developed method for creation of glass suction electrodes to allow simultaneous extra/intracellular recording

Education

  • University of Colorado Anschutz Medical Campus
    Doctor of Philosophy - PhD, Biomathematics, Bioinformatics, and Computational Biology
    2021 - 2027
  • Union College
    Bachelor of Science - BS, Bioinformatics
    2015 - 2019

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