PHD Student

• Sep 2021 - Present

Conditioning agent/preparator

• Feb 2021 - Aug 2021

Zootechnician/Engineer assistant

• Oct 2020 - Dec 2020

Laboratory animal house maintenance, animal monitoring, technical support for students' practicums. Technical support to a PhD project: histology (cryostat slicing, staining), dissections of rats. Laboratory animal house maintenance, animal monitoring, technical support for students' practicums. Technical support to a PhD project: histology (cryostat slicing, staining), dissections of rats.

Student Internship

• Feb 2020 - Aug 2020

Fundamental research. Subject 1 : Treatment of Parkinson’s disease by simultaneous blockade of DAT and D2-receptors. - Parkinson's model : intracerebral injection (stereotaxy) of 6-OHDA, to have a partial unilateral model of parkinsonian akinesia. - Behavioral test : CatWalk test, Stepping and Cylinder tests, to quantify akinesia and locomotion. - Toxicity test : to evaluate the lethal dose. - Immunohistochemistry : to confirm the validity of this model (reduction of dopamine neurons in the substantia nigra and striatum). - Data processing : statistical analysis. Report writing and oral presentation of this project. Subject 2 : Defining the brain circuits that interface hunger state with reward signaling to guide food consumption. Do food cue-modulated PVT (Thalamic Paraventricular Nucleus) neurons project to the nucleus accumbens ? - Optogenetic method (stereotaxy): for selective neuronal activation. - Single neuron neurophysiology. - Electrophysiology (stereotaxy): recordings of acurate nucleus, PVT and nucleus accumbens activities. - Behavioral test with conditioning : to follow neuronal responses to food cues. - Immunohistochemistry : to follow c-Fos expression. - Data processing : statistical analysis, to compare the effects of each drugs. Show less

Store Cashier

• Jun 2017 - Jan 2020

Student Internship

• Apr 2019 - Aug 2019

Fundamental research. Topic: Microglial reactivity: the impact of alpha-synuclein. - Primary cell cultures of glial cells; cell cultures of microglia. - Cell cultures treatments (pro-inflammatory mediators, alpha-synuclein). - Cell cytotoxicity analysis. - Analysis of alpha-synuclein structural profil. - Analyses of cell morphology, phagocytosis, gene expression, protein expression, and cell signalling pathways. Report writing and oral presentation. Fundamental research. Topic: Microglial reactivity: the impact of alpha-synuclein. - Primary cell cultures of glial cells; cell cultures of microglia. - Cell cultures treatments (pro-inflammatory mediators, alpha-synuclein). - Cell cytotoxicity analysis. - Analysis of alpha-synuclein structural profil. - Analyses of cell morphology, phagocytosis, gene expression, protein expression, and cell signalling pathways. Report writing and oral presentation.

Store Cashier

• Jun 2016 - Aug 2016