Frédéric Kohler

Cell Culture Associate at ExcellGene SA
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Contact Information
us****@****om
(386) 825-5501
Location
Sion, Valais, Switzerland, CH

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Experience

    • Switzerland
    • Biotechnology Research
    • 1 - 100 Employee
    • Cell Culture Associate
      • Jul 2021 - Present

    • United States
    • Biotechnology
    • 100 - 200 Employee
    • research Officer
      • Jan 2021 - Jun 2021

    • Germany
    • Biotechnology Research
    • 700 & Above Employee
    • BPS USP Technician
      • May 2020 - Dec 2020

    • Switzerland
    • Higher Education
    • 300 - 400 Employee
    • Master thesis - Engineering of a host cell line for manufacturing of recombinant proteins
      • Aug 2019 - Mar 2020

      - Vectors construction - Cultivation of different CHO cell lines - Adaptation, routine cultivation and batch culture in different media - Phenotype selection of cells in order to avoid clumping - Optimisation of transfection parameters - Screen of positive transformants - Vectors construction - Cultivation of different CHO cell lines - Adaptation, routine cultivation and batch culture in different media - Phenotype selection of cells in order to avoid clumping - Optimisation of transfection parameters - Screen of positive transformants

    • Switzerland
    • Higher Education
    • 400 - 500 Employee
    • Bachelor thesis - SUMO Protease Production and activity testing”
      • May 2018 - Aug 2018

      - Different vectors construction. One for the expression of the CBTb tagged with the SUMO protein in E. coli and another for the expression of the SUMO-protease in Pichia Pastoris. - Construction of a strain in E. coli producing the SUMO-CBTb - Screen of positive clone and batch cultivation - DSP on the desire protein - Construction of a strain in Pichia Pastoris producing the SUMO-protease - Screen of positive transformant using a BioLector - Different vectors construction. One for the expression of the CBTb tagged with the SUMO protein in E. coli and another for the expression of the SUMO-protease in Pichia Pastoris. - Construction of a strain in E. coli producing the SUMO-CBTb - Screen of positive clone and batch cultivation - DSP on the desire protein - Construction of a strain in Pichia Pastoris producing the SUMO-protease - Screen of positive transformant using a BioLector

  • Viteos SA
    • La Chaux-de-Fonds, Canton de Neuchâtel, Suisse
    • Apprentissage de laborantin en biologie
      • Aug 2010 - Aug 2013

      - Sampling and water analysis - Wastewater and purified water analysis - Participation in method development - Sampling and water analysis - Wastewater and purified water analysis - Participation in method development

  • Galvarex SA
    • Saint Imier
    • Apprenti galvanoplaste
      • Aug 2009 - Aug 2010

      - Production of electroplated endless wires tubes and watchmaking - Preparation and maintenance in the electroplating workshop - Production and regulation of the production line of mandrel - Machine operator on a centerless grinding machine - Production of electroplated endless wires tubes and watchmaking - Preparation and maintenance in the electroplating workshop - Production and regulation of the production line of mandrel - Machine operator on a centerless grinding machine

Education

  • HES-SO Haute école spécialisée de Suisse occidentale
    Master of Science in Life Sciences, Applied Biosciences
    2018 - 2020
  • HES-SO Valais-Wallis
    Bachelor of Science - BS, Biotechnologie
    2015 - 2018
  • Ceff Saint-Imier
    Maturité professionelle technique
    2014 - 2015
  • EF Executive Language Institute
    B2 FCE, English
    2013 - 2014
  • CPLN, Neuchâtel
    CFC de laborantin en biologie, Biochimie, biologie moléculaire, microbiologie, ...
    2010 - 2013
  • CIFOM La Chaux-de-Fonds
    Electrochimie
    2009 - 2010

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