Frédéric Kohler
Cell Culture Associate at ExcellGene SA- Claim this Profile
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Bio
Experience
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ExcellGene SA
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Switzerland
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Biotechnology Research
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1 - 100 Employee
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Cell Culture Associate
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Jul 2021 - Present
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Ichnos Sciences
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United States
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Biotechnology
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100 - 200 Employee
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research Officer
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Jan 2021 - Jun 2021
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Merck Life Science
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Germany
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Biotechnology Research
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700 & Above Employee
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BPS USP Technician
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May 2020 - Dec 2020
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HES-SO Haute école spécialisée de Suisse occidentale
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Switzerland
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Higher Education
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300 - 400 Employee
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Master thesis - Engineering of a host cell line for manufacturing of recombinant proteins
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Aug 2019 - Mar 2020
- Vectors construction - Cultivation of different CHO cell lines - Adaptation, routine cultivation and batch culture in different media - Phenotype selection of cells in order to avoid clumping - Optimisation of transfection parameters - Screen of positive transformants - Vectors construction - Cultivation of different CHO cell lines - Adaptation, routine cultivation and batch culture in different media - Phenotype selection of cells in order to avoid clumping - Optimisation of transfection parameters - Screen of positive transformants
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HES-SO Valais-Wallis
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Switzerland
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Higher Education
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400 - 500 Employee
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Bachelor thesis - SUMO Protease Production and activity testing”
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May 2018 - Aug 2018
- Different vectors construction. One for the expression of the CBTb tagged with the SUMO protein in E. coli and another for the expression of the SUMO-protease in Pichia Pastoris. - Construction of a strain in E. coli producing the SUMO-CBTb - Screen of positive clone and batch cultivation - DSP on the desire protein - Construction of a strain in Pichia Pastoris producing the SUMO-protease - Screen of positive transformant using a BioLector - Different vectors construction. One for the expression of the CBTb tagged with the SUMO protein in E. coli and another for the expression of the SUMO-protease in Pichia Pastoris. - Construction of a strain in E. coli producing the SUMO-CBTb - Screen of positive clone and batch cultivation - DSP on the desire protein - Construction of a strain in Pichia Pastoris producing the SUMO-protease - Screen of positive transformant using a BioLector
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Viteos SA
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La Chaux-de-Fonds, Canton de Neuchâtel, Suisse
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Apprentissage de laborantin en biologie
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Aug 2010 - Aug 2013
- Sampling and water analysis - Wastewater and purified water analysis - Participation in method development - Sampling and water analysis - Wastewater and purified water analysis - Participation in method development
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Galvarex SA
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Saint Imier
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Apprenti galvanoplaste
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Aug 2009 - Aug 2010
- Production of electroplated endless wires tubes and watchmaking - Preparation and maintenance in the electroplating workshop - Production and regulation of the production line of mandrel - Machine operator on a centerless grinding machine - Production of electroplated endless wires tubes and watchmaking - Preparation and maintenance in the electroplating workshop - Production and regulation of the production line of mandrel - Machine operator on a centerless grinding machine
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Education
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HES-SO Haute école spécialisée de Suisse occidentale
Master of Science in Life Sciences, Applied Biosciences -
HES-SO Valais-Wallis
Bachelor of Science - BS, Biotechnologie -
Ceff Saint-Imier
Maturité professionelle technique -
EF Executive Language Institute
B2 FCE, English -
CPLN, Neuchâtel
CFC de laborantin en biologie, Biochimie, biologie moléculaire, microbiologie, ... -
CIFOM La Chaux-de-Fonds
Electrochimie