Floriane BRETHEAU, PhD

Professionnelle de recherche at Cirris
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Contact Information
us****@****om
(386) 825-5501
Location
CA

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Experience

    • United States
    • Appliances, Electrical, and Electronics Manufacturing
    • 1 - 100 Employee
    • Professionnelle de recherche
      • Apr 2021 - Present

      Professionnelle de recherche en recherche fondamentale clinique Professionnelle de recherche en recherche fondamentale clinique

    • PhD Student
      • Oct 2015 - Apr 2021

      PhD project: Inhibition of interleukine-1 alpha as a neuroprotective therapy for spinal cord injury Main techniques: - Transgenic mouse models (15 different models) - Microscopy (2-photon, confocal,...) - Flow-cytometry - Primary cell culture (monocytes, astrocytes, oligodendrocytes, microglia, endothelial cells) PhD project: Inhibition of interleukine-1 alpha as a neuroprotective therapy for spinal cord injury Main techniques: - Transgenic mouse models (15 different models) - Microscopy (2-photon, confocal,...) - Flow-cytometry - Primary cell culture (monocytes, astrocytes, oligodendrocytes, microglia, endothelial cells)

    • France
    • Higher Education
    • 1 - 100 Employee
    • Engineer student
      • Sep 2012 - Jun 2015

      Multidiscplinary formation in Biotechnology, Master degree Multidiscplinary formation in Biotechnology, Master degree

    • Switzerland
    • Biotechnology Research
    • 700 & Above Employee
    • Intern in Pharma Research and Early Development, Infectious Diseases and Immunology Group
      • Jul 2014 - Feb 2015

      Industrial work placement, Roche Innovative Center Zurich, Schlieren, Switzerland Main project: Set up of functional immune cellular based assays to study and characterize B cell dysfunction in chronic hepatitis B, focusing on B cell phenotype, activation, proliferation and differentiation, using 10 color flow cytometry. Main techniques: - PBMCs and B cells isolation from human blood - 10-color flow cytometry - proliferation, activation and differentiation assays - ELISA Industrial work placement, Roche Innovative Center Zurich, Schlieren, Switzerland Main project: Set up of functional immune cellular based assays to study and characterize B cell dysfunction in chronic hepatitis B, focusing on B cell phenotype, activation, proliferation and differentiation, using 10 color flow cytometry. Main techniques: - PBMCs and B cells isolation from human blood - 10-color flow cytometry - proliferation, activation and differentiation assays - ELISA

    • France
    • Pharmaceutical Manufacturing
    • Work Placement
      • Jun 2013 - Aug 2013

      Industrial work placement Fluofarma , specialized in high throughput content screening, Pessac, France Projects: Set up of a Tissue Micro Array composed by 60 different Human Tumorous Cell lines and protein expression study in murine glioblastoma by immunohistochemistry. Main techniques: - Cell culture - Immunohistochemistry Industrial work placement Fluofarma , specialized in high throughput content screening, Pessac, France Projects: Set up of a Tissue Micro Array composed by 60 different Human Tumorous Cell lines and protein expression study in murine glioblastoma by immunohistochemistry. Main techniques: - Cell culture - Immunohistochemistry

    • Work Placement
      • Jun 2011 - Jul 2011

      - Study of obesogenic molecules impact on Danio rerio model - Study of obesogenic molecules impact on Danio rerio model

Education

  • Université Laval
    PhD Student, Molecular medicine
    2015 - 2020
  • ENSTBB
    Diplôme d'ingénieur, Biotechnologies
    2012 - 2015

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