Experience

Janssen Vaccines & Prevention B.V.

• Netherlands
• Pharmaceutical Manufacturing
• 1 - 100 Employee

• Senior Scientist

  • Nov 2014 - Present

  Managing of late-stage discovery and early development activities, i.e., transfer of vaccine candidates to Cell Line Development and CMC support (Flu, RSV, …). Operational and technical lead for external projects with research institutes, academic partners, potential business partners, and contract research organizations. Assist in antigen design and responsible for antigen screening and production, writing patents and scientific papers. Skills: Operational project management of late-stage discovery and early development activities, Antigen screening, Scientific publications, Patent writing. Show less



Crucell

• Biotechnology Research
• 1 - 100 Employee

• Scientist, Influenza Vaccine Discovery

  • Jan 2012 - Oct 2014

  Leading team of technicians responsible for production (USP & DSP) and characterization of proteins for pre-clinical evaluation. Design of viral fusion protein antigens, scientific publications and patents writing. Skills: Line management, Antigen design, Protein expression and biophysical characterization, Patent writing, Scientific publications. Leading team of technicians responsible for production (USP & DSP) and characterization of proteins for pre-clinical evaluation. Design of viral fusion protein antigens, scientific publications and patents writing. Skills: Line management, Antigen design, Protein expression and biophysical characterization, Patent writing, Scientific publications.



UMC Utrecht

• Netherlands
• Hospitals and Health Care
• 700 & Above Employee

• Postdoctoral Researcher

  • Sep 2007 - Dec 2011

  Identified and characterized Staphylococcus aureus and Pseudomonas aeruginosa secreted proteins by which the pathogens manage to escape the human immune system. Contributed to collaborative projects with AM-Pharma and Intervet/Schering-Plough Animal Health and developed a plug and play plasmid system for rapid evaluation of bacterial expression variables. Prof. Dr. Jos van Strijp, Infection & Immunity Skills: Molecular biology (cloning, site directed mutagenesis), Protein expression (bacterial systems) and purification, Protein characterization and biological assays (2D gel electrophoresis, ITC, MS, FACS), Antigen design. Show less



Utrecht University

• Netherlands
• Higher Education
• 700 & Above Employee

• PHD Student

  • Jul 2002 - Dec 2006

  Thesis: Structural studies on the enzymes of the complement convertases; factor B, C2a and the inhibitor SCIN The structures of recombinantly produced complement Factor B, C2a and Staphylococcal complement inhibitor protein were elucidated by X-ray crystallography. In combination with site-directed mutagenesis studies new insights in the structure and function of the central enzyme complex of the complement activation cascade were obtained. Prof. Dr. Piet Gros, Bijvoet Centre for Biomolecular Research Skills: Molecular biology (cloning, site directed mutagenesis), Protein expression (mammalian systems) and purification, Protein crystallization, X-ray dataset collection, Structure determination and interpretation. Show less