Fayeza Md Siraj, PhD
GMO and Allergen Analyst level 4 at BVAQ- Claim this Profile
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Bio
Credentials
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CPR/AED/First Aid
St John AmbulanceAug, 2023- Nov, 2024 -
Approved Arrangement Accreditation for AA Accredited Persons (Classes 1-8)
Australian Government Department of Agriculture, Water and the EnvironmentMay, 2023- Nov, 2024 -
Biosecurity Awareness
Australian Government Department of Agriculture, Water and the EnvironmentMay, 2023- Nov, 2024
Experience
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BVAQ
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Australia
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Food and Beverage Services
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100 - 200 Employee
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GMO and Allergen Analyst level 4
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Mar 2021 - Present
• Utilizing LIMS for tasks such as receiving samples, generating batches, and raising queries. • Employing different methods to extract samples based on ELISA KIT specifications. • Formulating buffers for both simple and enhanced extraction processes. • Conducting ELISA for a range of tests including gluten, total milk, egg, crustacea, hazelnut, various tree nuts, almond, sesame, peanut, walnut, lysozyme, and fish. • Performing ELISA testing with a variety of kits, such as Biofront, Immunolab Sensispec, Romer Fisher, Neogen, and Gliadin. • Operating Thunderbolt for Gluten testing. • Utilizing the Magellan ELISA plate reader to analyze results. • Creating in-house quality control materials for assays using raw materials to use as positive controls. • Handling biohazard and quarantine samples. • Regularly inspecting lab equipment to ensure proper functioning and maintenance. • Executing DNA extraction of milk samples using the complete 16 patho-proof mastitis kit. • Running PCR assays using ABI7500 software to identify micoplasma bovus and 15 other species in cow milk. • Performing DNA extraction from seeds, processed foods, oils, etc. using methods like Qiaxen, CTAB, and Haxene for GMO testing. • Managing seed counts, documentation, and conducting GMO tests as required by MPI standards. • Conducting PCR for GMO testing through Triple screen, Quattro, and Das methods Using AriaMax. • Compiling Laboratory Investigation Reports (LIR) and CAPA (Corrective and Preventive Action) reports. Calibration: • Calibrating various types of pipettes, including single-channel, automatic, or manual multi-channel pipettes. • Performing balance calibration across a range of volumes using standard weights. • Calibrating heat blocks using probes to ensure accurate tube heating. • Conducting nano calibrations to assess DNA quality and quantity. • Performing calibration on the ABI7500 PCR machine. Show less
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DTS Food Assurance
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Australia
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Food and Beverage Services
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1 - 100 Employee
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Microbiologist
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Mar 2016 - Feb 2021
Job Role ● Experienced and familiar working in a NATA accredited laboratory. ● Sampling powder/perishable food in a laminar hood following aseptic technique. ● Prepare composite sample from bulk numbers, quarantine sample preparation. ● Prepare samples using media and incubate to test . ● Environmental swabbing and monitoring. ● Placing orders for QC approved products, Inventory, Stock take. ● Maintaining OHS and SOPs, following MSDS and PPE. ● LabWare LIMS and ELN: data entry and result verification. Show less
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Konkuk University
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South Korea
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Higher Education
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200 - 300 Employee
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Postdoctoral Researcher
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Oct 2014 - Apr 2015
As a postdoctoral researcher, I focused on microarray analysis of tissues (Thymus, Spleen, Liver, Kidney) obtained from CMAH-KO pigs. The objective was to compare the gene ontology with that of wild-type pigs. Given the structural and genetic similarities between pigs and humans, pigs are considered potential candidates for organ transplantation in humans. Following the microarray analysis, I conducted in-silico investigations using DAVID software to identify specific differential gene ontology and KEGG pathways associated with immune-related disorders. Subsequently, I performed experimental validation studies based on the results of the in-silico analysis. Show less
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Kyung Hee University
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South Korea
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Ph.D
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2011 - 2014
● Cell culture and cell handling ● Cell viability checking via MTT, XTT tests and then reading plate in the ELISA reader. ● Preparing media for cell lines or solutions for experimental works. ● Disease specific assays on adipocyte cell line. Eg. Oil Red O staining, TG assay ● Treatment of medicinal plant on adipocytes to check efficacy. ● RNA isolation, cDNA synthesis, RT-PCR, qRT-PCR, agarose gel electrophoresis ● Experimental data analysis, scientific manuscript preparation and journal submission, Powerpoint presentation of regular work flow Show less
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Education
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Kyung Hee University
Doctor of Philosophy - PhD, Biotechnology -
University of Asia Pacific
Masters in Pharmaceutical Technology, Pharmacy -
University of Asia Pacific
Bachelor's degree, Bachelor of Pharmacy