Eshna Jash
Early Career Leadership Program at Genetics Society of America- Claim this Profile
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Bio
Experience
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Genetics Society of America
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United States
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Research Services
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1 - 100 Employee
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Early Career Leadership Program
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May 2022 - Present
o Responsible for hosting and editing “Genetics in our world” podcast interviewing scientists about their research and helping them communicate their findings with a wider audience o Creating and maintaining resources for scientists interested in science communication
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University of Michigan
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United States
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Higher Education
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700 & Above Employee
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Doctoral Researcher
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Sep 2018 - Present
Ann Arbor, Michigan, United States 1) Identifying direct targets of a C. elegans transcription factor (conserved in mammals) that is important for embryogenesis • Performed RNAseq and ChIPseq to identify targets of the transcription factor. Validated potential targets using RT-qPCR, RNAi and immunostaining experiments • Used CRISPR-Cas9 to generate mutants with endogenously-tagged proteins for immunoprecipitation experiments • Established pipelines in the lab for mRNA-seq analysis using R and python, as well as methods… Show more 1) Identifying direct targets of a C. elegans transcription factor (conserved in mammals) that is important for embryogenesis • Performed RNAseq and ChIPseq to identify targets of the transcription factor. Validated potential targets using RT-qPCR, RNAi and immunostaining experiments • Used CRISPR-Cas9 to generate mutants with endogenously-tagged proteins for immunoprecipitation experiments • Established pipelines in the lab for mRNA-seq analysis using R and python, as well as methods for RNA extraction, cDNA conversion and RT-qPCR • Received Youngman Research Fellowship for Fall 2022, and Rackham Graduate Research Fellowship for Fall 2019 2) Transcriptomic analysis of C. elegans embryos to show novel function for a gene in regulating cellular differentiation and plasticity • Performed RNAseq and GSEA analysis in mutants to show regulation of HMTs that are important for sex determination and cellular differentiation in early embryogenesis • Further characterized changes in epigenetic marks in these mutants using immunostaining 3) Effects of nuclear RNAi in C. elegans dosage compensation • Performed RT-qPCR to measure transcriptional changes in genes important for dosage compensation in mutants with defective nuclear RNAi pathways
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Graduate Student Instructor
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Aug 2019 - Mar 2022
Ann Arbor o Winter 2022: BIO306-Genetics Lab. Taught lab classes, graded assignments, exams, lab reports, and held office hours o Fall 2019: BIO305-Introduction to Genetics. Taught discussion sections for students, helped write quizzes and held office hours o Summer 2020: BIO225-Animal Physiology, helped transition class materials such as quizzes and lectures online, including editing and transcription of video lectures
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Vertex Pharmaceuticals
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United States
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Biotechnology Research
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700 & Above Employee
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Computational Genomics Intern
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May 2023 - Aug 2023
Boston, Massachusetts, United States - Analysed large-scale RNAseq datasets for differential gene expression and differential splicing - Performed statistical analyses and created data visualizations - Developed analysis plan and presented results to cross-functional teams
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Nucleate
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United States
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Biotechnology Research
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100 - 200 Employee
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Founding Member - Michigan Chapter
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Jan 2022 - Jun 2022
Ann Arbor, Michigan, United States Nucleate is a start-up accelerator based out of Boston • As a founding member for the Michigan chapter, I interfaced with various academic and business entities in the area (eg. professors, tech transfer office, VCs, local start-up leaders) • Compiled a report identifying the challenges faced by biotech start-ups in Michigan and how Nucleate can help bridge these gaps • Organized networking events to connect key stakeholders in academia and the industry
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miLEAD Consulting
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United States
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Market Research
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1 - 100 Employee
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Project Manager
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May 2021 - Apr 2022
Led a team of consultants in conducting primary research for a molecular diagnostics company and prepared a white paper targeted towards primary physicians
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Life Sciences Consultant
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Apr 2020 - May 2021
Worked as part of team for several projects involving market research and competitive landscape analysis, developed and delivered recommendations on business
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Shiv Nadar University
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India
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Higher Education
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700 & Above Employee
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Senior Thesis, Sehrawat Lab
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Aug 2017 - May 2018
Greater Noida Studying the effect of EPAC1 in angiogenesis and metastasis of triple-negative breast cancer • Maintained all mammalian cell culture stocks in the lab, including triple-negative breast cancer cell lines, HER-1+ breast cancer cell lines, and primary endothelial cell line • Performed western blots, MTT assays, angiogenesis assays and cell migration assays to study the effect of EPAC1 inhibition on breast cancer • Received Opportunity for Undergrad Research (OUR) Grant • Resulted in a… Show more Studying the effect of EPAC1 in angiogenesis and metastasis of triple-negative breast cancer • Maintained all mammalian cell culture stocks in the lab, including triple-negative breast cancer cell lines, HER-1+ breast cancer cell lines, and primary endothelial cell line • Performed western blots, MTT assays, angiogenesis assays and cell migration assays to study the effect of EPAC1 inhibition on breast cancer • Received Opportunity for Undergrad Research (OUR) Grant • Resulted in a 3rd author research paper and 2 review papers Won Poster Award at the National Conference on "Recent Trends in Cell and Molecular Biology Research", Shiv Nadar University
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Undergraduate Researcher, Sehrawat Lab
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Aug 2016 - Jul 2017
Greater Noida Received grant of ₹30,000 under Dr. Seema Sehrawat (Associate Professor, Shiv Nadar University). Exploring the role of cilostazol and its derivatives in maintenance of neuroplasticity after ischemic stroke.
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Undergraduate Researcher, Chakrabarty Lab
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Aug 2015 - Jul 2016
Greater Noida Received grant of ₹30,000 under Dr. Anindita Chakrabarty (Assistant Professor, Shiv Nadar University). Cloning of shRNA constructs into lentiviral vectors for transfection into breast cancer cells: Targeting molecular mechanisms for resistance against PI3K inhibitors.
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Microbiology Intern
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May 2016 - Jul 2016
Kolkata Under Dr. Alok K Sil (Associate Professor and HOD, Department of Microbiology). Exploring the mechanism of signal transduction that causes cell death after exposure to cigarette smoke using yeast as a model system.
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Education
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University of Michigan
Doctor of Philosophy - PhD, Molecular, Cellular and Developmental Biology -
Shiv Nadar University
Bachelor of Science, Biotechnology