Experience

CARGO Therapeutics

• United States
• Biotechnology Research
• 1 - 100 Employee

• Scientist I

  • Aug 2023 - Present

• Associate Scientist

  • Mar 2022 - Jul 2023

  • Establishment of in-house cloning capabilities and optimization of workflow to increase production of viral plasmid generation. • Production of CAR-T using viral transduction and characterization via flow cytometry, cytokine secretion and serial killing. • Optimization of CAR-Ts by identifying splice variants via RT-PCR and removal of splice sites.



Anwita Biosciences Inc

• United States
• Biotechnology Research
• 1 - 100 Employee

• Associate Scientist

  • Aug 2021 - Feb 2022

  • Led 2 nanobody discovery projects from construction of phage display library to antibody characterization and development (Titer, Stability, Purity, Epitope binning, Humanization). • Optimization of phage display techniques to identify high affinity antibodies. • Generation of project reports for patent applications/IND filing.

• Senior Research Associate

  • Jan 2020 - Jul 2021

  • Protein engineering to optimize stability, yield, and affinity of cytokine fusion proteins. • Verification of protein-protein interactions and affinity measurements by BLI. • Development of cytokine fusion protein chromatography methods. • Optimization of protein formulation for long term storage and stability.

• Research Associate

  • Apr 2018 - Jan 2020

  • Design and molecular cloning of antibodies and fusion proteins via PCR, Digestion/Ligation, Gibson Assembly. • Cell Culture maintenance and transfections of CHO cells. • Purification of antibodies and fusion proteins with AKTA including: Affinity, Ion-Exchange, SEC, HIC • Characterization of proteins via SDS-PAGE, HPLC, DLS, MALS, ELISA and LAL Assay.



University of California, Santa Cruz

• United States
• Higher Education
• 700 & Above Employee

• Junior Specialist

  • Jun 2016 - Apr 2018

  • Studied Ribosome structure and function by creating mutant ribosomes via mutagenesis, expression, purification, and in-vitro assays to test their functional capabilities in translation and translocation. • Procurement of lab supplies and maintenance of buffers and medias. • Coordinated with Environmental Health and Safety Department to meet lab safety regulations by proper disposal of hazardous/radioactive waste and other safety specifications. • Training new laboratory members on… Show more • Studied Ribosome structure and function by creating mutant ribosomes via mutagenesis, expression, purification, and in-vitro assays to test their functional capabilities in translation and translocation. • Procurement of lab supplies and maintenance of buffers and medias. • Coordinated with Environmental Health and Safety Department to meet lab safety regulations by proper disposal of hazardous/radioactive waste and other safety specifications. • Training new laboratory members on laboratory practices and molecular biology techniques.

• Lab Assistant

  • May 2015 - Jun 2016

  Part-time undergraduate research at the Noller laboratory in the Department of MCD Biology and Center for Molecular Biology of rRNA