Experience

Tacit Therapeutics

• United States
• Biotechnology Research
• 1 - 100 Employee

• Senior Scientist

  • Sep 2023 - Present

• Scientist II

  • Jun 2023 - Sep 2023



University of California, Berkeley

• United States
• Higher Education
• 700 & Above Employee

• Postdoctoral Scholar

  • Sep 2022 - May 2023

• PHD Student

  • Sep 2015 - Aug 2022

  - Used ribosome profiling, western blot, 5' RACE, polysome analysis, and metabolic labeling to determine the mechanism driving the "neighboring gene effect", a long observed but little understood off-target effect in selection mediated loss of function mutants. Our work demonstrated bidirectional promoter activity inherent to eukaryotic promoters within genome inserted cassettes caused this off-target effect, thus providing a means to prevent it in future studies. We then designed and shared an… Show more - Used ribosome profiling, western blot, 5' RACE, polysome analysis, and metabolic labeling to determine the mechanism driving the "neighboring gene effect", a long observed but little understood off-target effect in selection mediated loss of function mutants. Our work demonstrated bidirectional promoter activity inherent to eukaryotic promoters within genome inserted cassettes caused this off-target effect, thus providing a means to prevent it in future studies. We then designed and shared an improved set of selection cassettes for use in Saccharomyces cerevisiae which prevent this off target effect.