Senior Scientist

• Dec 2021 - Present

- Planning, writing protocols and performing laboratory activity and analysis for in vitro characterization of immuno-therapeutical potential of antibodies targeting malignant haematological cell models.- GCP-based PK assessment on blood samples from patients enrolled in clinical trials.- Planned, managed and followed up collaborations with CROs for the execution of mouse experiments (therapy and PK studies) and tissue cross-reactivity (TCR) studies for the development of therapeutic antibodies.- initiated, managed and followed up academic collaborations for testing of the therapeutic antibodies in the Nordic Nanovector discovery pipeline.- Writing and revising scientific reports.- contributed to patent submission with results and reports from in vitro and in vivo antibody characterization- presented results on B-cell targeting antibodies in a scientific poster at the 64th American Society for Haematology annual meeting in New Orleans (December 10-13th 2022)-Planned, written protocols, optimized and tested cytotoxic potential of novelradioimmunotherapy (RIT) in discovery phase.- coordinated the R&D team on the use of electronic laboratory notebook (ELN) (written SOP, contact person with ELN provider) Show less

Lab Manager and Senior Scientist

• Apr 2021 - Dec 2021

Senior Scientist tasks:- Planning, writing protocols and performing laboratory activity and analysis for in vitro characterization of immuno-therapeutical potential of antibodies targeting malignant haematological cell models.- GCP-based PK assessment on blood samples from patients enrolled in clinical trials. Planned and performed method qualification for the instrument used for PK assessment.- Basic expertise with radioactive labelling of antibodies and immuno-reactive fraction (IRF) assessment.- Planned, managed and followed up collaborations with CROs for the execution of mouse experiments for the development of therapeutic antibodies.- Written and revised scientific reportsLab manager duties:- planned and organized periodic maintenance of laboratory equipment. - implemented electronic laboratory notebook (ELN) to be used for recording of R&D experimental activity Show less

Associate Member

• Mar 2020 - Present

Principal Engineer

• Feb 2021 - Feb 2021

Collaboration with research scientists for the design and cloning of fusion protein constructs, cell culture methods and lab management. Collaboration with research scientists for the design and cloning of fusion protein constructs, cell culture methods and lab management.

Postdoctoral Fellow

• May 2016 - Oct 2020

Working in Jørgen Wesche's "Molecular Biology of Sarcomas" Research group. My main project aims at understanding the role of oncogenic mutations of FGFR4 in Rhabdomyosarcoma, by discovering signalling determinants affecting proliferation and migration. During this project I have: § Conceived, troubleshooted and analyzed quantitative proteomics studies (BioID pull down, sample preparation for LC-MS/MS) to discover FGFR4 oncogenic signaling partners in Rhabdomyosarcoma (RMS). § Collaborated with Proteomic experts (Proteomics Core Facility - OUS) in the identification of FGF-induced phosphorylations of signaling proteins. § Collaborated with Genomic experts and bioinformaticians for analysis and critical interpretation of RNA-seq and Exome-seq data § Characterized FGFR4 regulation through cell surface labelling and internalization, confocal imaging and protein-protein interaction (IP) analyses. § Successfully achieved stable expression of engineered proteins by designing and optimizing DNA cloning, point mutagenesis and cell transfections in cancer cell lines. § Profiled oncogenic addition and in vitro drug sensitivity by analysis of viability (MTS), proliferation, adhesion and migration (IncuCyte and ImageJ) upon RNAi and pharmacological treatment in RMS cells. § Promoted the fame of the team by presenting in an international meeting, collaborating on one published paper and writing 2 first-author manuscripts. § Taken active leadership in the organization of seminars and networking events improving collaborations, social interaction and career development of fellow researchers (ICR Post-doc forum and lab retreat). Show less

Consultant

• Jul 2018 - Jul 2018

Surveying methods used by different clinical laboratories for cancer diagnosis and classification. Surveying methods used by different clinical laboratories for cancer diagnosis and classification.

PhD student

• Nov 2011 - Apr 2016

Working in Toni Hurtado's "Breast Cancer" Research group on the functional characterisation of CTCF and FOXA1 transcription factors in breast and endometrial cancer models. § Effectively managed multiple research projects investigating Estrogen dependent gene regulation and the genomic effects of growth factor signaling on breast cancer endocrine therapy response. § Extensive hands-on expertise on various genomic methods (ChIP, FAIRE, DNA and RNA isolations and qPCR). § Successfully generated NGS DNA libraries and performed bioinformatic analyses to profile genome-wide protein-chromatin binding (ChIP-seq) and chromatin accessibility (FAIRE-seq) in breast cancer cell lines. § Collaborated with a bioinformatic team to elucidate SNP variant effects on mRNA expression, chromatin landscape and breast cancer prognosis. § Gained supervision experience by training one Master’s student for cell lab procedures and bioinformatics. § Contributed to the success of the team with an oral presentation in an international meeting, a “Best poster” award at an international course, 3 publications as main author and 2 papers as co-author. Show less

Undergraduate student

• Sep 2008 - Jul 2011

Working in Paola Defilippi's and Sara Cabodi's research group, under the supervision of Pilar Camacho-Leal. My main project elucidated the interplay between p130Cas and Estrogen Receptor in branching morphogenesis by using ex vivo organotypic culture from mouse mammary gland. Here I have: § Introduced in the lab the isolation and ex vivo 3D culture of primary mammary organoids from mice to study adult mammary gland development and breast cancer cell biology. § Developed 3D immunofluorescence and western blot protocols to describe different histological phenotypes and their signaling. Expertise with staining for flow cytometry (FACS) to describe epithelial cell populations. § Contributed to the generation of conditional knock-out mice by collecting and PCR genotyping samples from different organs; kept accurate record of animals used for in vivo and ex vivo studies. Show less

Private Tutor

• Sep 2006 - Jun 2009

One-to-one classes of Latin, Mathematics, Physics and English aimed to help 4 high school and university students to raise their exam grades. One-to-one classes of Latin, Mathematics, Physics and English aimed to help 4 high school and university students to raise their exam grades.