Experience

CNCR, Center for Neurogenomics & Cognitive Research

• Netherlands
• Research Services
• 1 - 100 Employee

• PhD candidate

  • Feb 2020 - Present

• Research assistant

  • May 2017 - Feb 2020

  Making 3D reconstructions of neurons (as during the internship). Reconstructions are made from in-vitro recorded neurons, from the temporal cortex, amygdala and hippocampus of humans. Reconstructions were also made for rat and mice neurons. I have introduced bachelor and master students to the process of making these reconstructions, and corresponding programs used for quantification.

• Research Intern

  • Feb 2017 - May 2017

  Explore the morphology of human pyramidal cells in the cortex, and compare this morphology among different layers. 3D reconstructions of these neurons were made using Neurolucida, and the reconstructions were validated using Surveyor, ImageJ, Adobe Ilustrator and Neuroexplorer. Practical work was rewarded with a 9, report with a 7.5.



Utrecht University

• Netherlands
• Higher Education
• 700 & Above Employee

• Research Internship

  • Mar 2019 - Aug 2019

  CRISPR/Cas9 Developing a toolbox to localize the beta subunits of calcium channels in the presynaptic terminal. This toolbox was created with use of CRISPR/Cas9 knockin constructs, combined with a cre-lox system. We have tested this in primary cultured hippocampal rat neurons, with positive outcome, and visualized this with confocal microscopy. In addition, we have assessed subsynaptic localization using STORM microscopy. CRISPR/Cas9 Developing a toolbox to localize the beta subunits of calcium channels in the presynaptic terminal. This toolbox was created with use of CRISPR/Cas9 knockin constructs, combined with a cre-lox system. We have tested this in primary cultured hippocampal rat neurons, with positive outcome, and visualized this with confocal microscopy. In addition, we have assessed subsynaptic localization using STORM microscopy.



Anatomy & Neurosciences at VUmc

• Faculty of Science

• MSc Research intern

  • Feb 2018 - Aug 2018

  Department Clinical Neuroanatomy and Biobanking. Main tasks: - Localize the "Nucleus basalis of Meynert" (nbM) on MRI (AD vs CN) - Obtain mean diffusivity (MD) and fractional anisotropy (FA) using DTI - Stain the nbM of both AD and CN for (p-)Tau, abeta, microglia and ChAT positive neurons Grade: 7.5 Department Clinical Neuroanatomy and Biobanking. Main tasks: - Localize the "Nucleus basalis of Meynert" (nbM) on MRI (AD vs CN) - Obtain mean diffusivity (MD) and fractional anisotropy (FA) using DTI - Stain the nbM of both AD and CN for (p-)Tau, abeta, microglia and ChAT positive neurons Grade: 7.5



Cafe Bubbels Purmerend

• Hospitality
• 1 - 100 Employee

• Barpersoneel

  • Mar 2015 - 2018



Studievereniging Anguilla

• Mentor new upcoming first year students

  • Aug 2016 - Sep 2016

  Guide upcoming first year students through the VU and Amsterdam. Explain the principle of studying Health and life sciences, and everything that comes with it. Take students on multiple tours throughout Amsterdam, to get them familiar with the city as well.

• Penningmeester commissie

  • Sep 2015 - Sep 2016

  (vrijwillig)

• Voorzitter activiteitencommissie

  • Sep 2014 - Sep 2015

  (vrijwillig)