Eleonora Manitta
Postdoctoral Researcher at Novo Nordisk Foundation Center for Basic Metabolic Research- Claim this Profile
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Italiano Native or bilingual proficiency
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Inglese Professional working proficiency
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Francese Professional working proficiency
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Experience
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Novo Nordisk Foundation Center for Basic Metabolic Research
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Denmark
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Research Services
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100 - 200 Employee
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Postdoctoral Researcher
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Dec 2022 - Present
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PHD Student
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Sep 2018 - Present
PhD thesis title: Effect of parental environmental exposures on the epigenome and mechanisms of epigenetic inheritance. Main techniques: - DNA methylation profiling by RRBS, pyrosequencing, and amplicon sequencing- Mammalian cell culture- Embryonic stem cell culture and neural differentiation- Epigenome editing by CRISPR-based methods- Isolation and purification of spermatozoa and processing for DNA/RNA analysis- RNA sequencing- smallRNA sequencing
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Research Assistant
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Nov 2017 - Aug 2018
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Università degli Studi di Torino
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Italy
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Education Administration Programs
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700 & Above Employee
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Research Assistant
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Jan 2017 - Jul 2017
Project: Analysis of long non-coding RNA expression and function in breast cancer cell lines Main techniques: - Mammalian cell culture - Gene knockdown by siRNA - Proliferation assays - qRT-PCR - ChIP-qPCR Project: Analysis of long non-coding RNA expression and function in breast cancer cell lines Main techniques: - Mammalian cell culture - Gene knockdown by siRNA - Proliferation assays - qRT-PCR - ChIP-qPCR
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Master´s thesis internship
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Feb 2016 - Sep 2016
Thesis project: Analysis of the epigenetic modification 5-formylcytosine in colon mucosa biopsies. Main techniques: - DNA purification - Preparation of sequencing libraries for RRBS, fCAB-Seq, MAB-Seq, and amplicon sequencing - PCR - Sample preparation for HPLC Thesis project: Analysis of the epigenetic modification 5-formylcytosine in colon mucosa biopsies. Main techniques: - DNA purification - Preparation of sequencing libraries for RRBS, fCAB-Seq, MAB-Seq, and amplicon sequencing - PCR - Sample preparation for HPLC
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Internship
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Jan 2014 - Jun 2014
Project: Analysis of Single Nucleotide Polymorphisms of cytokine genes and genes involved in xenobiotic metabolism, and cytogenetic analysis of peripheral blood lymphocytes in hospital workers. Main techniques: - DNA extraction and purification - PCR-based SNP detection - Lymphocyte culture - Karyotyping - Micronucleus assay Project: Analysis of Single Nucleotide Polymorphisms of cytokine genes and genes involved in xenobiotic metabolism, and cytogenetic analysis of peripheral blood lymphocytes in hospital workers. Main techniques: - DNA extraction and purification - PCR-based SNP detection - Lymphocyte culture - Karyotyping - Micronucleus assay
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Education
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Università degli Studi di Torino
Laurea Magistrale LM, Biologia Cellulare e Molecolare -
Università degli Studi di Torino
Laurea triennale, Scienze Biologiche