Eleonora Manitta

Postdoctoral Researcher at Novo Nordisk Foundation Center for Basic Metabolic Research
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Location
Copenhagen, Capital Region, Denmark, DK
Languages
  • Italiano Native or bilingual proficiency
  • Inglese Professional working proficiency
  • Francese Professional working proficiency

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Experience

    • Denmark
    • Research Services
    • 100 - 200 Employee
    • Postdoctoral Researcher
      • Dec 2022 - Present

    • PHD Student
      • Sep 2018 - Present

      PhD thesis title: Effect of parental environmental exposures on the epigenome and mechanisms of epigenetic inheritance. Main techniques: - DNA methylation profiling by RRBS, pyrosequencing, and amplicon sequencing- Mammalian cell culture- Embryonic stem cell culture and neural differentiation- Epigenome editing by CRISPR-based methods- Isolation and purification of spermatozoa and processing for DNA/RNA analysis- RNA sequencing- smallRNA sequencing

    • Research Assistant
      • Nov 2017 - Aug 2018

    • Italy
    • Education Administration Programs
    • 700 & Above Employee
    • Research Assistant
      • Jan 2017 - Jul 2017

      Project: Analysis of long non-coding RNA expression and function in breast cancer cell lines Main techniques: - Mammalian cell culture - Gene knockdown by siRNA - Proliferation assays - qRT-PCR - ChIP-qPCR Project: Analysis of long non-coding RNA expression and function in breast cancer cell lines Main techniques: - Mammalian cell culture - Gene knockdown by siRNA - Proliferation assays - qRT-PCR - ChIP-qPCR

    • Master´s thesis internship
      • Feb 2016 - Sep 2016

      Thesis project: Analysis of the epigenetic modification 5-formylcytosine in colon mucosa biopsies. Main techniques: - DNA purification - Preparation of sequencing libraries for RRBS, fCAB-Seq, MAB-Seq, and amplicon sequencing - PCR - Sample preparation for HPLC Thesis project: Analysis of the epigenetic modification 5-formylcytosine in colon mucosa biopsies. Main techniques: - DNA purification - Preparation of sequencing libraries for RRBS, fCAB-Seq, MAB-Seq, and amplicon sequencing - PCR - Sample preparation for HPLC

    • Internship
      • Jan 2014 - Jun 2014

      Project: Analysis of Single Nucleotide Polymorphisms of cytokine genes and genes involved in xenobiotic metabolism, and cytogenetic analysis of peripheral blood lymphocytes in hospital workers. Main techniques: - DNA extraction and purification - PCR-based SNP detection - Lymphocyte culture - Karyotyping - Micronucleus assay Project: Analysis of Single Nucleotide Polymorphisms of cytokine genes and genes involved in xenobiotic metabolism, and cytogenetic analysis of peripheral blood lymphocytes in hospital workers. Main techniques: - DNA extraction and purification - PCR-based SNP detection - Lymphocyte culture - Karyotyping - Micronucleus assay

Education

  • Università degli Studi di Torino
    Laurea Magistrale LM, Biologia Cellulare e Molecolare
    2014 - 2016
  • Università degli Studi di Torino
    Laurea triennale, Scienze Biologiche
    2011 - 2014

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