Ela Dudek
VP of Research and Development at Future Fields- Claim this Profile
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Bio
Experience
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Future Fields
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Canada
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Biotechnology Research
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1 - 100 Employee
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VP of Research and Development
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May 2022 - Present
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Team Lead, Protein Purification
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Aug 2020 - Present
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University of Alberta
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Canada
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Higher Education
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700 & Above Employee
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Research Associate
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Nov 2016 - Aug 2020
Developing and commercializing a recombinant protein vaccine against Hepatitis C Virus, currently in pre-clinical development. Integral member of protein purification team to purify antigen from mammalian cells.• Responsible for molecular cloning, expression and purification of protease used in purification • Responsible for experimental design and execution of process development studies • Developed knowledge of cGMPs as applied to process development and scale up• Responsible for viral clearance study • Experience writing technical documents including development /tech transfer reports, batch records, and standard operating procedures• Experience working in cleanroom environments• Contributed to process risk assessments• Collaborate with scientific team and quality/regulatory team to develop and implement biochemical tools or assays to characterize product quality attributes• Work in collaboration with colleagues in pilot and manufacturing scale operations to devise scale-up strategies for protein purification Show less
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Postdoctoral Researcher
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Jan 2011 - Oct 2016
Postdoctoral Researcher (Alberta Innovates Health Solutions), University of Alberta, Department of Biochemistry, Edmonton, Canada, Projects: Calreticulin mutants in human sudden death, Calnexin interactions with cytoplasmic and plasma membrane proteins, Mapping ATP-binding site of calnexin• Overexpressed and purified proteins using prokaryotic and eukaryotic expression systems.• Molecular interaction analysis using advanced techniques like Surface Plasmon Resonance and Microscale Thermophoresis.• Worked with mammalian cell cultures, overexpressed proteins for purification and/or visualization using microscopy techniques, including FRET measurements of ion fluxes in single cells.• Established and use CISPR/cas9 technologies. Show less
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Nencki Institute of Experimental Biology PAS
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Poland
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Research
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200 - 300 Employee
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Doctor of Philosophy (PhD) in Biochemistry, PhD thesis defended with honors
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2005 - 2010
PhD studies at the Laboratory of Molecular Motors, Department of Biochemistry, Nencki Institute of Experimental Biology Studies on protein biochemistry. Ph. D thesis: " Ncd – mitotic kinesin’s subunits interaction during mechanochemical cycle" Studies on protein biochemistry. Duties: • independently and collaboratively design and execute experiments in support of research projects - developed protocols and purified homo and heterodimers of kinesin Ncd, made of mutated subunits and characterized them using advanced techniques like TIRF microscopy, FRET techniques - established TIRF microscopy technology in the Nencki Institute of Experimental Biology • analyzing the resulting data • maintaining detailed records • preparing reports and presentations based on the findings • critical review of scientific press • organized, maintained, and ordered lab equipment, supplies and chemicals Core skills: • ability to work well with others & independently • time management skills and a strong attention to detail • organizational skills Show less
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