Eddy Risseeuw

Genomic Solutions Specialist at NRGene Ltd.
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Location
Saskatoon, Saskatchewan, Canada, CA

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Experience

    • Israel
    • Biotechnology
    • 1 - 100 Employee
    • Genomic Solutions Specialist
      • Apr 2021 - Present

    • United States
    • Wellness and Fitness Services
    • 1 - 100 Employee
    • Research Technician
      • Jan 2020 - Nov 2020

      Developed tissue culture methods for cannabis propagation and cryo preservation. Developed tissue culture methods for cannabis propagation and cryo preservation.

    • Technical Officer
      • 2018 - 2019

      • Developed and applied novel DNA library preparation methods for ATAC-seq and DAP-seq. The positions of open chromatin and of transcription factors binding sites were identified throughout the entire canola and wheat genomes using Next Generation Sequencing. In particular the binding sites of the cold tolerance inducing CBF transcription factors were analyzed in the wheat genome. • Developed and applied novel DNA library preparation methods for ATAC-seq and DAP-seq. The positions of open chromatin and of transcription factors binding sites were identified throughout the entire canola and wheat genomes using Next Generation Sequencing. In particular the binding sites of the cold tolerance inducing CBF transcription factors were analyzed in the wheat genome.

    • Molecular Plant Breeder
      • 2014 - 2018

      • Accomplished a 50-80% glucosinolate reduction in elite Brassica carinata breeding lines by identifying the low glucosinolate ‘Bronowski’ alleles in canola and corresponding alleles in Brassica juncea followed by interspecific crosses and marker assisted introgression. • Developed an efficient and economical method for simultaneous SNP, InDel and PAV genotyping for crops with a complex genome. The principles of the KASP and microsattelite PCR methods were combined and multiplexed, followed by capillary electrophoresis and analysis using the GeneMarker software• Introgression of the Ogura hybrid system and herbicide tolerance in carinata. Setup, maintenance and harvest of the nursery and the yield trials. Data collection and statistical analysis.

    • Research Officer
      • 2012 - 2013

      • Executed an industrial project in partnership with Valent Biosciences Inc. on the validation of new variants of the plant hormone abscisic acid. ABA receptors were crystallized and diffracted in the synchrotron. • Executed an industrial project in partnership with Valent Biosciences Inc. on the validation of new variants of the plant hormone abscisic acid. ABA receptors were crystallized and diffracted in the synchrotron.

    • Professional Research Associate
      • 2010 - 2012

      • Assembled a multi-disciplinary research team for a new project on arthritis. A novel auto-antigen based vaccine was developed, produced in tobacco and validated in mice for reducing arthritis. • Assembled a multi-disciplinary research team for a new project on arthritis. A novel auto-antigen based vaccine was developed, produced in tobacco and validated in mice for reducing arthritis.

    • Research Officer
      • 2004 - 2009

      • Managed and executed contract research for DOW Agrosciences and demonstrated gene editing in canola using the zinc-finger nuclease technology. • Characterized transcription factors regulating embryogenesis in Arabidopsis. • Managed and executed contract research for DOW Agrosciences and demonstrated gene editing in canola using the zinc-finger nuclease technology. • Characterized transcription factors regulating embryogenesis in Arabidopsis.

    • United States
    • Biotechnology Research
    • Senior Scientist
      • 2003 - 2004

      • Researched the interaction between the mucosa and small anti-inflammatory proteins in chickens. A method was developed to harvest and culture primary intestinal epithelial cells from chicken embryo’s. • Researched the interaction between the mucosa and small anti-inflammatory proteins in chickens. A method was developed to harvest and culture primary intestinal epithelial cells from chicken embryo’s.

    • Research Associate
      • 1997 - 2003

      • Researched regulatory proteins involved in plant development and growth regulation. • Published research findings in recognized international journals. • Researched regulatory proteins involved in plant development and growth regulation. • Published research findings in recognized international journals.

Education

  • Edwards School of Business
    Master's certificate, Project Management
    2019 - 2020
  • Leiden University
    PhD, Molecular biology
    1991 - 1996
  • Wageningen Agricultural University
    MSc, Plant Breeding
    1985 - 1991

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