Experience

Technical University of Munich

• Germany
• Research Services
• 700 & Above Employee

• Lab Leader, Prof. Johannes Buchner lab

  • Apr 2021 - Present

  • Actively carrying out research in the area of protein biochemistry • In charge for implementation of best laboratory working practices• Supervising lab organization

• Postdoctoral Scientist, Prof. Johannes Buchner Lab

  • Feb 2015 - Present

  • Studying mechanistic details of the molecular interactions between the tumor suppressor protein p53, steroid hormone receptors and cellular chaperone systems• Investigating protein conformational dynamics by modern single molecule fluorescent techniques• Protein structure-function investigations• Characterization of dynamic multi-protein complexes, protein-DNA binding• Cloning, mutagenesis, expression and purification of proteins by AKTA FPLC systems• Protein characterization and analysis• Development of assays to study protein conformational dynamics and specific protein interactions in multi-protein complexes• Autonomous designing and planning of experiments and analysis• Managing inter-disciplinary collaborations• Maintenance of spectrophotometers and fluorescence dyes• Supervising student interns, master thesis projects• Publishing and Reviewing of scientific work• Writing of weekly progress reports and presentation of results in team meetings



Indian Institute of Technology, Delhi

• Higher Education
• 700 & Above Employee

• PhD Research Fellow

  • Aug 2008 - Nov 2014

  • Investigated spontaneous and chaperone assisted folding mechanism of a multi-domain protein • Studied thermodynamics and kinetics of protein folding (calculation of free energy of protein folding, kinetic rate constants)• Characterized folding pathway intermediates and developed refolding strategies• Characterized proteins by structural probes (fluorescence, CD spectroscopy) and enzymatic activity assays• Planned, designed, executed and managed independently my research project• Initiated and coordinated productive research collaborations



National Institute of Communicable Diseases

• 1 - 100 Employee

• Summer Intern

  • Jun 2005 - Jul 2005

  • Diagnosis of tuberculosis using conventional and molecular diagnostic techniques like PCR, ELISA • Diagnosis of tuberculosis using conventional and molecular diagnostic techniques like PCR, ELISA