Experience

Am Uniklinikum Leipzig

• Germany
• Research Services
• 1 - 100 Employee

• Researcher PHD Student

  • Aug 2019 - Dec 2019

  Leipzig Area, Germany



OVGU Magdeburg

• Researcher PHD Student

  • Apr 2014 - Jul 2019

  Magdeburg Isolation of single cells from murine kidneys for Single Cell Sequencing Isolation of glomeruli and tubular epithelial cells from murine kidneys Lentiviral transfection and transduction, viral-particles preparation and infection of mammalian cell lines Maintaining mice colonies (T1DM, STZ-model), maintaining urine collection data and analyzing proteinuria; collecting blood and plasma and preserving organs for ex vivo analyses Tail-vein, intraperitoneal, subcutaneous injections of… Show more Isolation of single cells from murine kidneys for Single Cell Sequencing Isolation of glomeruli and tubular epithelial cells from murine kidneys Lentiviral transfection and transduction, viral-particles preparation and infection of mammalian cell lines Maintaining mice colonies (T1DM, STZ-model), maintaining urine collection data and analyzing proteinuria; collecting blood and plasma and preserving organs for ex vivo analyses Tail-vein, intraperitoneal, subcutaneous injections of drugs in mice Proximity Ligation assay ex vivo and in vitro (cells and tissue sections) IF/IHC of paraffin-embedded and cryo- kidney sections ChiP-sequencing of ER stress/UPR promoter regions to identify transcription factors in mice diabetic kidneys qRT-PCRs and RT2 Profiler arrays (Qiagen) PAS-staining and quantification of extracellular matrix depositions using ImageJ/Fiji Quantification of glomerular basement membrane width using ImageJ/Fiji Designing new and innovative tools/ techniques for imaging Scheduling and organizing journal clubs and lab presentations Established regular PCR-based mycoplasma testing of cell lines in culture Maintaining and monitoring the cell culture incubators and hoods Supervising and training medical students in wet lab techniques Designing and planning new research projects Show less



Leibniz Institute on Aging - Fritz Lipmann Institute (FLI)

• Germany
• Research Services
• 100 - 200 Employee

• Research Assistant

  • Apr 2013 - Jan 2014

  Jena Area, Germany Preparing and maintaining keratinocytes raft cultures Confocal microscopy imaging and analysis of the angle of polarized cell divisions in raft cultures



Charité - Universitätsmedizin Berlin

• Germany
• Hospitals and Health Care
• 700 & Above Employee

• Molecular Medicine Masters student

  • Oct 2010 - Oct 2012

  Lab internship (4 months) at the Max Delbrück Center for Molecular Medicine (MDC), Berlin-Buch in Colorectal Cancer Lab internship (6 month) at the Deutsches Herzzentrum Berlin (DHZB) in Atherosclerosis and Nerve Growth Factor in Smooth Muscle Cells. Masters Thesis project in Colorectal Cancer at the MDC, Berlin-Buch



Brandeis University

• United States
• Higher Education
• 700 & Above Employee

• Rosenstiel Basic Medical Sciences Research Center, Lab manager/ Research assistant

  • May 2009 - Jan 2011

  Cryopreservation and maintenance of mammalian cell lines Preparation of bacterial and mammalian tissue culture media and cytokines Trained in mouse genotyping, flow cytometry and bioactivity assays Conducted training of new graduate and undergraduate students Environmental & chemicals safety incharge Maintenance of laboratory instruments such as bench top flow cytometer and Beckman Coulter Counter, spreadsheets and databases, troubleshooted problems associated with equipments and… Show more Cryopreservation and maintenance of mammalian cell lines Preparation of bacterial and mammalian tissue culture media and cytokines Trained in mouse genotyping, flow cytometry and bioactivity assays Conducted training of new graduate and undergraduate students Environmental & chemicals safety incharge Maintenance of laboratory instruments such as bench top flow cytometer and Beckman Coulter Counter, spreadsheets and databases, troubleshooted problems associated with equipments and techniques Show less



Gerstenzang Science Library, Brandeis University

• Student Manager

  • Sep 2005 - May 2009

  Managing the main desk and supervising, training new student employees at the Science library Organizing books by their catalogue numbers Reserving books for the science courses per semester as instructed by Professors Maintaining a clean and quite environment at the library Closing and locking the library after late night shifts on weekdays and weekends Checking the gate count statistics