Senior Scientist

• May 2022 - Present

Scientist 2/Senior Scientist

• Sep 2020 - May 2022

Scientist within the Functional Genomic Screening team, working as both project lead and as part of a team to conduct pooled CRISPR screens (drug-gene and gene-gene) in a wide range of cell lines. Experimental techniques used:- Pooled CRISPR screens (CRISPRko, CRISPRa, CRISPRi) using either cell proliferation or phenotypic readouts - Mammalian cell culture- Lentiviral production and cell line transduction- Molecular cloning- Stable cell line generation- Genomic DNA extraction, PCR and NGS sample preparation- Assay development and optimisation- Flow cytometryAdditional skills and responsibilities:- Client interactions – data presentations and answering queries - Data analysis and production of reports/presentations- Actively involved in process improvements e.g. using R skills to improve NGS analysis pipeline (automated graphs/plots)- Operational project lead – use organisation and troubleshooting skills to ensure projects kept to timelines- Team member – assist with lab work and share knowledge/expertise as needed

Postdoctoral Research Associate

• Oct 2018 - Sep 2020

Research project investigated the effect of the bacterial pathogen Salmonella on gene expression within host immune cells.Experimental techniques used include:- Next-generation sequencing (NGS) library preparation - RNA-Seq, Ribo-Seq (ribosome profiling)- Nucleic acid extraction/purification - Proficient in RNA handling- PCR/qPCR- Cell culture (cell lines and primary cells)- Immunofluorescence microscopy, flow cytometry- Cell based assaysDeveloped and improved bioinformatic skills:- Experienced with using R for data analysis and presentation- Comfortable with command line tools, and bash/C shell scripting (tcsh) - e.g. for the analysis of RNA-Seq experimentsAdditional skills and responsibilities:- Lab management, including health and safety compliance and maintaining lab inventory- Supervising and advising junior lab members

Research Associate

• Oct 2017 - Sep 2018

Worked to understand the virulence mechanisms of the bacterial pathogen, Salmonella Typhi. Experimental techniques used include:- bacterial culture, aseptic technique- construction of bacterial strains- cell culture (cell lines)- plate-reader based assays Worked to understand the virulence mechanisms of the bacterial pathogen, Salmonella Typhi. Experimental techniques used include:- bacterial culture, aseptic technique- construction of bacterial strains- cell culture (cell lines)- plate-reader based assays

Summer Intern

• Jul 2012 - Aug 2012

Eight week research project working on improving the existing genome annotation of Xanthomonas campestris using RNA sequencing data. Gained experience in Gbrowse, Artemis and BLAST.Additional training in bioenterprise, science communication, bioinformatics, plant pathology and critical evaluation of scientific literature. Eight week research project working on improving the existing genome annotation of Xanthomonas campestris using RNA sequencing data. Gained experience in Gbrowse, Artemis and BLAST.Additional training in bioenterprise, science communication, bioinformatics, plant pathology and critical evaluation of scientific literature.