Experience

China Jiliang University

• China
• Higher Education
• 1 - 100 Employee

• Lecturer

  • Sep 2019 - Present

  Stress biology in legume crops.CRISPR/Cas 9 in vegetable crops Stress biology in legume crops.CRISPR/Cas 9 in vegetable crops



International Crops Research Institute for the Semi-Arid Tropics (ICRISAT) Hyderabad-502 324, Telang

• India
• Education Administration Programs
• 1 - 100 Employee

• Visiting Scientist

  • Jul 2016 - Aug 2019

  Moving a step closer towards allergen-free groundnuts using OMICS (MABC and CRISPR/CAS9) approaches.Transcriptome profiling to understand the pre-harvest aflatoxin contamination (PAC) mechanism in groundnut seeds in response to A. flavus infection Genome-wide discovery of non-coding RNAs for IVSC resistance Moving a step closer towards allergen-free groundnuts using OMICS (MABC and CRISPR/CAS9) approaches.Transcriptome profiling to understand the pre-harvest aflatoxin contamination (PAC) mechanism in groundnut seeds in response to A. flavus infection Genome-wide discovery of non-coding RNAs for IVSC resistance



University of Hyderabad

• India
• Higher Education

• Postdoctoral Fellow

  • Feb 2011 - Jun 2016

  Project: Genome Editing using CRISPR/CAS9 in tomato CRISPR/CAS9 modules design, Development of CRISPR vectors, Generation of transgenicplants, Transient studies, Molecular and genetic analysis. Project: Sequence-Indexed mutations Library in the Tomato Genome. Cloning of Ac and Ds element bearing construct in binary vector. Raising of Transgenic lines harboring Ac element, Ds element and empty vector. Molecular characterization of transgenics lines through PCR, qPCR, Standardized TAIL PCR and Southern blotting.Guided grad and undergrad students for research work. Project: Generation of Hypermutable lines in tomato through silencing of MMR gene Cloning of MSH2 gene through Gateway™ System for Making of RNAi vector. Generation of transgenics lines and molecular characterization of transgenics plants Project: Validate the function of gene through VIGS. Making of VIGS vector to know the function of gene in tomato.



Indian Institute of Millets Research (IIMR) Rajendranagar, Hyderabad, India.

• Senior Research Fellow

  • Jul 2005 - Jan 2011

  Project: Developing low HCN accumulating forage sorghum (Sorghum bicolor (L.) Moench.) through antisense approach.  CYP79A1 cDNA gene was isolated and cloned into transformation vector in antisense orientation for genetic transformation of sorghum to reduce dhurrin content. Transgenic plants carrying the antisense CYP79A1 gene were regenerated, characterised and promising events were advanced (now in T4 generation).  Transgenics were confirmed by PCR and Southerns, gene expression studied by Rt-PCR and qPCR, HCN content determined biochemically. The HCN content was reduced in the transgenics (range of 17 -179 µg/g) with a mean of 104 µg/g compared to 192 µg/g in the control Guided master students for research; maintaining chemicals & consumables in the labProject: Down-regulation of somatic embryogenesis receptor kinase (SERK) gene in sorghum ovules. Three members of sorghum SERK gene family were identified and sorghum SERK1 cDNA target for RNA interference was identified and cloned for down-regulation. Ovule-specific promoter was isolated from Arabidopsis and cloned into Agrobacterium transformable vector; transgenics were developed to verify ovule-specific promoter function in sorghum



Defence Research Development & Establishment (DRDE), Gwalior, India

• Project Assistant

  • Jan 2005 - Jun 2006

  Rroject: Development of PCR based detection system of Vibrio cholera.” Multiplex PCR system was developed to detect the Vibrio cholera strain.

• Graduate Trainee

  • Feb 2004 - Dec 2004

  Project: Production of antibodies against Recombinant staphylococcal Enterotoxin B gene. Cloning of Enterotoxin B gene in expression Vector, Express the protein, Purification of the protein, Injection this protein in Mice and Rabbit to produce Antibodies against this protein