Dorothy Brach

Senior Scientist at NextRNA Therapeutics
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us****@****om
(386) 825-5501

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5.0

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Kamala Kesavan

Dorothy is a skilled biologist in assay development, detail-oriented execution, and analysis of both cell biology and biochemical assays. She has a great positive-attitude and took the initiative to research, demo and incorporate new liquid handling and robotic technologies. It was great to work with Dorothy and I highly recommend her work.

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Experience

    • United States
    • Biotechnology Research
    • 1 - 100 Employee
    • Senior Scientist
      • Nov 2022 - Present

    • United States
    • Biotechnology Research
    • 1 - 100 Employee
    • Senior Scientist
      • May 2022 - Nov 2022

    • United States
    • Biotechnology Research
    • 1 - 100 Employee
    • Scientist
      • Sep 2018 - May 2022

    • Principal Research Associate
      • Apr 2015 - Sep 2018

    • Sr. Research Associate
      • Jul 2013 - Mar 2015

    • United States
    • Biotechnology Research
    • 1 - 100 Employee
    • Research Associate
      • May 2012 - Jun 2013

      •Worked on several cancer targets and maintained various human cancer cell lines: MDA-MB-231, SJSA-1, MCF-7, H23, H196, Raji, FaDu, MV-4-11, and RS4;11 as some examples. •Developed and conducted routine viability, apoptosis and LDH cell based assays and HTRF enzyme assays for screening. •Routinely looked at downstream markers by western blot and ELISA techniques. •Analyzed protein-protein interactions by IP in intact cells and in cell lysates. •Gained basic knowledge of stapled peptide chemistry. Show less

    • Switzerland
    • Pharmaceutical Manufacturing
    • 700 & Above Employee
    • Scientific Associate II
      • Jan 2012 - May 2012

      •Performed immunoprecipitations to determine interacting proteins with target protein. •Gained experience with mammalian cell culture and human breast cancer cell lines. •Developed TR-FRET based enzyme assays.

    • Scientific Associate II
      • Mar 2010 - Dec 2011

    • Scientific Associate I
      • Jul 2007 - Mar 2010

      •Constructed clones, over expressed and purified various proteins for assay purposes. •Developed individual enzyme assays and medium throughput enzyme screens. •Supported Microbiology lab in various tasks including routine MIC testing, PAE testing, bactericidal vs. bacteriostatic determination, strain identification using API strips and 16s sequencing.•Developed and implemented system for the tracking of hundreds of compounds.•Assisted as HSE (Health, Safety, and Environment) representative for our department. •Analyzed all data and regularly presented to project teams. •Demonstrated and instructed other colleagues in study protocol. Show less

    • Research Assistant in the laboratory of Dr. Susan B. Leschine (Department of Microbiology)
      • Jan 2005 - Jun 2007

      • Isolated, characterized, extracted DNA, and ran PCR on isolates from Pulque. • Performed reducing sugar, protein, and enzyme assays on Clostridium phytofermentans. • Analyzed DNA of Clostridium phytofermentans using the BLAST program. • Used HPLC to analyze fermentation products of C. phytofermentans. • Presented research findings to laboratory members during meetings. • Isolated, characterized, extracted DNA, and ran PCR on isolates from Pulque. • Performed reducing sugar, protein, and enzyme assays on Clostridium phytofermentans. • Analyzed DNA of Clostridium phytofermentans using the BLAST program. • Used HPLC to analyze fermentation products of C. phytofermentans. • Presented research findings to laboratory members during meetings.

Education

  • University of Massachusetts Amherst
    MS, Microbiology
    2006 - 2007
  • University of Massachusetts Amherst
    BS, Microbiology
    2002 - 2006

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