Experience

Cellecta, Inc.

• United States
• Biotechnology Research
• 1 - 100 Employee

• Computational Biologist

  • Sep 2021 - Present

  Mountain View, California, United States



Novogene Corporation

• Bioinformatics Engineer

  • Sep 2019 - Sep 2021

  Sacramento, California Area 1. Developed, maintained, and operated various in-house bioinformatics pipelines using various opensource bioinformatics softwares, Python, R packages, HTML/CSS, JavaScript, shell script, MySQL, MongoDB, workflow description language (WDL). Transcriptome(RNAseq): Hisat2, STAR, trinity, featurecount, DESeq2, edgeR, Whole Genome Sequencing(WGS) and Whole Exon Sequencing (WES): BWA-MEM, GATK, samtools, breakdancer, delly, freec, muTect/Strelka 10x… Show more 1. Developed, maintained, and operated various in-house bioinformatics pipelines using various opensource bioinformatics softwares, Python, R packages, HTML/CSS, JavaScript, shell script, MySQL, MongoDB, workflow description language (WDL). Transcriptome(RNAseq): Hisat2, STAR, trinity, featurecount, DESeq2, edgeR, Whole Genome Sequencing(WGS) and Whole Exon Sequencing (WES): BWA-MEM, GATK, samtools, breakdancer, delly, freec, muTect/Strelka 10x Single cell RNAseq: cellranger, seurat Amplicon and Metagenomics: Qiime2, SOAPdenovo, megahit 2. Performed customized bioinformatics data analysis requested by various clients. 3. Troubleshooted cases efficiently and collaborated seamlessly with project manager team and technical support team. 4. Managed large scale sequencing data and delivered data to clients using different tools, such ftp, sftp, aws, rclone et al. Show less



Louisiana State University

• United States
• Higher Education
• 700 & Above Employee

• Research Assistant

  • Aug 2012 - May 2019

  Baton Rouge, Louisiana Area Oct. 2015-May. 2019 Project: Reduction of Phakopsora pachyrhizi infection of soybean through host induced gene silencing and spray induced gene silencing Methods involved: construction of HIGS construct, optimality condition of biolistic particle delivery systems, ELISA, RT-PCR, qPCR, dsRNA in vitro synthesis, small RNA sequencing, bioinformatics analysis of small RNA sequencing (fastqc, cutadapt, bowtie, basic linux command lines, R package for data summary and visualization)… Show more Oct. 2015-May. 2019 Project: Reduction of Phakopsora pachyrhizi infection of soybean through host induced gene silencing and spray induced gene silencing Methods involved: construction of HIGS construct, optimality condition of biolistic particle delivery systems, ELISA, RT-PCR, qPCR, dsRNA in vitro synthesis, small RNA sequencing, bioinformatics analysis of small RNA sequencing (fastqc, cutadapt, bowtie, basic linux command lines, R package for data summary and visualization), manuscript preparation etc. Oct. 2014-May. 2019 Project: Soybean rust resistance related proteins functional study Methods involved: construction of VIGS construct, optimality condition of biolistic particle delivery systems, ELISA, RT-PCR, qPCR, small RNA sequencing, mRNA sequencing, bioinformatics analysis of small RNA sequencing data (fastqc, cutadapt, bowtie, basic linux command line, R package for data summary and visualization ) and mRNA sequencing data (fastqc, hisat2, R package for DEG), DAB staining, SEM, TEM, manuscript preparation etc. Aug. 2013-Aug. 2015: Project: RNA-Seq analysis of soybean recombinant-isogenic line carrying rust-resistant and -susceptible alleles Methods involved: SEM&TEM, DAB staining, RT-PCR, qPCR, RNA-seq, bioinformatics analysis (fastqc, bowtie2, RUM, R package for DEG), manuscript preparation, etc. Aug. 2012- Aug. 2013 Project: Soybean PR10 promoter related transcript factors study Methods involved: nucleus protein extraction, probe design, protein pull down, Show less