Dominique Gutierrez
Graduate Research Assistant at CUNY Advanced Science Research Center- Claim this Profile
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English Native or bilingual proficiency
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Spanish Native or bilingual proficiency
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German Elementary proficiency
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French Elementary proficiency
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Bio
Experience
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Advanced Science Research Center, GC/CUNY
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United States
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Research
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100 - 200 Employee
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Graduate Research Assistant
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Apr 2017 - Present
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The Graduate Center, City University of New York
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United States
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Higher Education
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500 - 600 Employee
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Graduate Research Assistant
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Sep 2016 - Present
Graduate Student in the Department of Biochemistry Graduate Student in the Department of Biochemistry
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The University of Texas at El Paso
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United States
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Higher Education
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700 & Above Employee
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Undergraduate Research Assistant
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Dec 2014 - Aug 2016
The SUMO-Influenza Laboratory for Cellular and Molecular Virology; PI: Dr. Germán Rosas-Acosta Project: Effects Mediated by the Global Increase in Cellular SUMOylation (GICS) Caused by early Nonstructural Viral Protein (NS1) SUMOylation During Influenza A Viral (IAV) Infection Activities: Purified various recombinant single domain antibody fragments (VHHs) expressed in E.Coli and performed functionality assays, Mentored trainees in the purification of recombinant proteins, Collected data through the use of immunofluorescence microscopy Skills: SDS-PAGE, western blot, bacterial culture, affinity chromatography, protein dialysis, protein purification, recombinant protein design, plasmid DNA purification, restriction analysis, miRNA and plasmid transfections, plasmid cloning by PCR, antibody conjugation, immunofluorescence staining, confocal microscopy, IAV viral infections, mammalian cell culture, mammalian cell lysis and protein extraction, ultra-density centrifugation Show less
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Columbia University
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United States
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Higher Education
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700 & Above Employee
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Amgen Summer Research Scholar
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May 2015 - Aug 2015
Amgen Scholar and Undergraduate Research Assistant in the laboratory of Dr. Joachim Frank Project: Transcription-Translation Coupling Complex Activities: Studied 70S ribosome expression in both wild-type and mutant E.coli strains, Purified 70S ribosome through the utilization of sucrose gradients, Investigated if a minimal coupling complex, RNAP-NusG-70S ribosome, could form in vitro Skills: native gel, ribosome purification, negatively stained cryo-EM sample and grid preparation Amgen Scholar and Undergraduate Research Assistant in the laboratory of Dr. Joachim Frank Project: Transcription-Translation Coupling Complex Activities: Studied 70S ribosome expression in both wild-type and mutant E.coli strains, Purified 70S ribosome through the utilization of sucrose gradients, Investigated if a minimal coupling complex, RNAP-NusG-70S ribosome, could form in vitro Skills: native gel, ribosome purification, negatively stained cryo-EM sample and grid preparation
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Education
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The Graduate Center, City University of New York
Doctor of Philosophy (Ph.D.), Biochemistry -
The University of Texas at El Paso
Bachelor’s Degree, Cellular and Molecular Biochemistry