Experience

Number 8 bio

• Australia
• Biotechnology
• 1 - 100 Employee

• Senior Metabolic Engineer

  • Aug 2022 - Present

  Sydney, New South Wales, Australia



Bondi Bio

• Australia
• Biotechnology Research
• 1 - 100 Employee

• Strain Engineer

  • May 2019 - Aug 2022

  Sydney, New South Wales, Australia Engineering cyanobacteria for the production of high-value compounds for the food, flavour and fragrance industry. Established a genetic engineering pipeline to efficiently and reliably transform marine and freshwater Cyanobacteria with improved turnaround times and success rates. Generated strains to produce diverse terpenoids for the cosmetic and aquaculture industry. Improved yields of first proof-of-concept strains several fold using iterative metabolic pathway… Show more Engineering cyanobacteria for the production of high-value compounds for the food, flavour and fragrance industry. Established a genetic engineering pipeline to efficiently and reliably transform marine and freshwater Cyanobacteria with improved turnaround times and success rates. Generated strains to produce diverse terpenoids for the cosmetic and aquaculture industry. Improved yields of first proof-of-concept strains several fold using iterative metabolic pathway engineering. Tested performance of strains in medium scale photobioreactors (up to 6L) Show less



Macquarie University

• Australia
• Higher Education
• 700 & Above Employee

• Associate Researcher

  • Feb 2019 - Apr 2019

  Sydney, Australia Protein expression and purification for the construction of nanoscale drug delivery systems.



Macquarie University

• Australia
• Higher Education
• 700 & Above Employee

• PHD Candidate

  • Dec 2015 - Dec 2018

  Sydney, Australia The aim of this 3-year PhD project was to demonstrate a biological conversion of highly abundant waste sources into valuable platform chemicals. During this project several enzymes were discovered, characterised and finally assembled into a synthetic pathway which converts spent coffee grounds into lactic acid. The pathway is based on a putative oxidative mannose metabolism potentially present in thermophilic archaea. A low number of enzymatic steps, cofactor recycling and the use of low-cost… Show more The aim of this 3-year PhD project was to demonstrate a biological conversion of highly abundant waste sources into valuable platform chemicals. During this project several enzymes were discovered, characterised and finally assembled into a synthetic pathway which converts spent coffee grounds into lactic acid. The pathway is based on a putative oxidative mannose metabolism potentially present in thermophilic archaea. A low number of enzymatic steps, cofactor recycling and the use of low-cost substrate make this pathway attractive for further improvement and future bioprocessing systems. Show less



FGen

• Internship

  • Jul 2015 - Nov 2015

  Basel Area, Switzerland Creating and screening of bacterial/fungal/yeast libraries, Production efficiency improvement of different industrial strains.



The University of Freiburg

• Germany
• Research Services
• 700 & Above Employee

• Scientific Assistant

  • Nov 2014 - Feb 2015

  preparation and conceptualisation of a Bachelor's Studies practical course

• Master Student

  • Feb 2014 - Dec 2014

  "PsrR1 - a photosynthesis regulating RNA" Investigated the role of non-coding RNA PsrR1 on photosynthetic functions and its mechanism of target donwregulation in the cyanobacterium Synechocystis sp. PCC 6803 Conducted independent research on non-coding RNA in cyanobacteria using techniques such as molecular cloning, RNA and Protein analysis e.g. RT-PCR, Northern/Western Blot, polysome profiling, physiological measurements e.g. oxygen evolution, chlorophyll quantification.



University of Heidelberg

• Research Services
• 700 & Above Employee

• Bachelor Student

  • Feb 2012 - Sep 2012

  "Signal transduction in yeast pheromone pathway upon pre-stimulation" Investigated the phenotypic response of the yeast pheromone MAPK pathway Conducted high throughput fluorescence microscopy experiments and examined molecular cloning strategies to label proteins flourescently

• Scientific Assistant

  • Oct 2010 - Jan 2012

  Quantifying changes in NF-kB in mice affected with Duchenne Muscular Dystrophy (DMD) work with mammalian proteins; performing protein analysis (e.g. SDS-gel electrophoresis, Western Blot)



University of Oregon

• United States
• Higher Education
• 700 & Above Employee

• Research Scholar

  • Sep 2011 - Oct 2011

  "Thermodynamics of acetoclastic methanogenesis in natural environments" Interrogated the requirement of aceacetoclastic methanogenesis in various natural environments. Worked with anaerobic bacteria; analyzed metabolites with high pressure liquid and gas chromatography (HPLC, GC)