Dorothy Keine, PhD

Medical Writer/Founder at 3Prime Medical Writing, LLC
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Location
Durham, North Carolina, United States, US

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I worked with Dorothy at uMETHOD Health, a small health-services start-up. Dorothy was a very valuable contributor to our team who brought a wealth of knowledge, insight, and expertise to our company in many medical-related areas. At start-ups, we all have to wear many hats and Dorothy was no exception. Dorothy provided guidance and insight into human biology and health, medicine, genomics, research studies, and regulations. She demonstrated a unique ability to concisely explain complex topics in understandable terms, but could easily convey those same concepts to researchers or doctors in their terms. Dorothy worked with our team to analyze and tune our processing, and wrote a number of research studies and papers that were presented at conferences or published in peer reviewed journals. Her work was thorough, careful, insightful, and accurate. She played an invaluable role in shaping the character, credibility, and strong scientific basis of our company. As a member of the team, Dorothy also took on other required roles and performed them exceptionally. She was our HIPAA officer, defined our patient on-boarding process, crafted many of our medical questionnaires, crafted most of the information we presented to potential clinic partners and patients, and acted as a liaison with clinic partners. She also managed all the references to the research behind our work and reviewed new research for applicability for inclusion in our work. She worked extremely well with our entire team and demonstrated incredible grace under difficult start-up pressures - and I never once saw her miss a proper Oxford comma. I have great respect for Dorothy and appreciation for her knowledge and abilities. I would work with Dorothy again without hesitation and I am certain she will be a strong, capable contributor in any setting.

Dorothy is a detailed-oriented, hard working, team player who can leverage her scientific background to quickly become an integral part of any team. At Intstrux, she was able to contribute day-1 as an Assistant Account Executive for an oncology account. She picked up the science effortlessly and helped manage the account with little supervision. It was a pleasure having Dorothy on our team, and she would make a great addition to any organization looking to have a smart, dedicated and medically-savvy professional.

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Experience

    • United States
    • Writing and Editing
    • 1 - 100 Employee
    • Medical Writer/Founder
      • Jan 2019 - Present

      3Prime creates accurate and well-written scientific and medical deliverables customized to meet your needs. Whether you’re communicating with experts, sales teams, or patients, we craft the story and approach to reach your audience. We work with all clients, from Fortune 500 pharma companies, start-ups, and non-profits in a wide array of therapeutic areas.Writing services include publications (manuscripts, posters), product support (slide decks, SRLs, literature searches, blog posts), educational materials for physicians and MSLs, training modules for sales and MSL teams, and academic books and book chapters.Please message me to inquire about availability.

    • United States
    • Medical and Diagnostic Laboratories
    • 1 - 100 Employee
    • Medical Affairs Manager
      • Sep 2014 - Nov 2018

      •Nation-wide physician liaison•Authored and presented papers and posters for publication•Edited creative and publications for scientific rigor and brand compliance•Collaborated with scientific advisory board to develop clinical study methodologies for grants•Developed campaigns and materials for education, PR, and marketingAchievements:•Brought in initial physician and patient customers•2018 SBIR grant application project lead•Designed and implemented educational and sales outreach to physicians and patients•Created and carried out clinical training programs for physicians and staff•Developed all initial materials, website, and social media•Developed and ran business operations

    • United States
    • Advertising Services
    • 1 - 100 Employee
    • Assistant Account Executive
      • Mar 2014 - May 2014

      -Collaborated with clients and key opinion leaders to optimize digital marketing strategy and develop timely, quality content for clients- Supported account leads on all projects- Managed internal team on projects- Ensured client expectations are met and managed- Edited projects for quality assurance

    • Pakistan
    • Advertising Services
    • Scientific Affairs Associate
      • Sep 2013 - Jan 2014

      -Collaborated with clients and key opinion leaders to optimize medical strategy and develop timely, quality scientific and clinical content for pharmaceutical and non-profit organizations- Scientific editorial writing included (but was not limited to) slide kits, data mining, virtual advisory board meeting preparations, video scripts, review articles, newsletters- Client counsel included establishing/maintaining relationship of trust with clients, identifying unmet needs, disease and cause marketing, and offering professional/technical leadership across multiple diverse projects

    • Research Technician
      • Jun 2012 - Jun 2013

      The amyloid-beta peptide accumulates in the brain of Alzheimer’s disease patients and initiates disease onset. Our lab studies the cellular pathways that regulate the formation and clearance of amyloid-beta. I am the lead technician performing in vivo microdialysis in mouse models of AD; this technique enables us to study how amyloid-beta changes every hour over several days in response to genetic, drug, or behavioral manipulation. The amyloid-beta microdialysis technique was originally developed in our lab and is only used by 7 other groups in the world; including Case Western Reserve and various other universities who visited Washington University so I could teach them this technique. In addition to performing our own experiments, I am also responsible for organizing, coordinating, and executing research projects in collaboration with academic and corporate groups. Techniques used: In vivo Aβ microdialysis, small animal stereotaxic surgery, intracerebral injections, drug administration to mice, sandwich ELISA, custom construction of Electroencephalography probes, and data analysis.

    • Research Intern
      • May 2011 - Aug 2011

      Huntington’s disease derives from a polyglutamine expansion in the huntingtin gene. This expansion results in the misfolding and aggregation of the protein in the cell where it becomes neurotoxic. Accurate quantification of the protein in in vitro is difficult. I constructed a huntingtin protein with a Cyc-Cys-Gly-Gly motif attached to either the amino or the carboxyl terminal of the gene so that aggregates could then be labeled using the biarsenical reagent FlAsH-EDT2. This reagent binds only to a tetracysteine motif which the huntingtin aggregates reconstituted, coordinating with FlAsH-EDT2 and fluorescing. Wild type huntingtin, which does not aggregate, did not fluoresce. Techniques used: PCR, ligation, designed primer, gel electrophoresis, bacterial transformation, plasmid purification, transfection, cell culture, western blot,

    • United States
    • Veterinary Services
    • Laboratory Technician
      • May 2009 - Aug 2010

      The Human Microbiome Project is a study to develop a reference of the microbial genome sequences that colonize the human body. The goal of the reference is to learn what microbes are present and colonizing in both health and disease states in order to better personalize medicine and nutrition. The microbes are identified by sequencing the 16s ribosomal RNA, chosen for its stability and conservation between different species of bacteria and Achaea. I prepared samples for sequencing through amplification and purification of samples, which I then used in transforming E. coli competent cells. Techniques used: PCR, gel electrophoresis, DNA purification, DNA ligation, electroporation.

Education

  • University of Brighton
    Doctor of Philosophy - PhD
    -
  • Boston University
    Master’s Degree, MS in Health Communication
    2014 - 2016
  • Hendrix College
    Bachelor of Arts, Biochemistry and Molecular Biology
    -
  • Incarnate Word Academy
    -

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