Dimosthenis Grapsas
Biologist at Bioiatriki Healthcare Group- Claim this Profile
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English Professional working proficiency
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French Limited working proficiency
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Greek Native or bilingual proficiency
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Bio
Experience
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Bioiatriki Healthcare Group
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Greece
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Hospitals and Health Care
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400 - 500 Employee
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Biologist
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Jun 2022 - Present
A permanent laboratory position in the Department of Electrophoreses- Daily use of the Sebia Capillary 2 Flex Piercing Electrophoresis Analyzer, Capillarys 3 Tera ImmunoFixation Electrophoresis (IFE) Analyzer and IFE Capillarys 3 Octa Analyzer for analysis of blood serum patient samples.- Assessment and validation of serum protein electrophoresis graphs. - Monthly preparation of Normal and Hypergamma control samples for two-level Quality Control and preparation of cleaning solution for tri-weekly cleaning of the analyzer's capillaries.- Weekly agarose gel electrophoresis of lipoproteins, LDH isoenzymes and alkaline phosphatases along with scanning of produced gels and editing of curves in the Platinum 6 computer software- Analysis of CSF and serum samples via IsoElectric Focusing for determination of Multiple Sclerosis (MS) disease- Extensive serum sample handling, worklist creation and patient sample searching on two Laboratory Information Systems (LIS), Biomed and SLiS.- Daily ImmunoFixation Electrophoresis (IFE) and Bence - Jones electrophoresis of urine samples with the use of SAS - 3 analyzer and SAS - 4 stainer.- Handling of Pasteur pipettes, vortex machines- Good Laboratory Practices Show less
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Biologist
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Jul 2021 - Jun 2022
A permanent laboratory position in the Department of Haematology- Daily use of the Abbott Alinity h-series haematological analyzer for determination of general blood test parameters: white cell type, red blood cell type and platelet count. Troubleshooting for analytical issues derived from damaged samples e.g. containing blood clot or inadequate quantity etc.- Extensive use of the Sysmex haematological analyzer and stainer for measuring general blood test parameters.- Everyday handling of the Siemens BCS XP analyzer for measuring the following coagulation parameters: QUICK (Prethrombin Time), fibrinogen, aPTT and D-Dimers. Supplementary use of the Siemens BFT II spectrophotometer for measurement of the first three aforementioned coagulation parameters. - Implementation of the wet lab techniques of direct and indirect Coombs. Upon positive result of indirect Coombs, determination of patient serum dilution in which negative result emerges through titration.- Use of the Siemens PFA-100 analyzer for measurement of platelet functionality.- Identification of patient blood group and Rhesus type (positive or negative) in whole blood samples using the classic "smear on a glass" technique.- Handling of pippetes, vortex machines and centrifuges- Good Laboratory Practices (GLP) Show less
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Locus Medicus S.E. Diagnostics Centre
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Cholargos, Attiki, Greece
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Internship Trainee
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Feb 2021 - Apr 2021
Training on the sperm diagram technique in a male infertility laboratory: - Assessment of sperm motility with the use of a lab counter through optical microscopy - Measuring of sperm count using the Neubauer haemocytometer - Assessment of sperm morphology - Special treatment of biological fluids (semen) Training on the sperm diagram technique in a male infertility laboratory: - Assessment of sperm motility with the use of a lab counter through optical microscopy - Measuring of sperm count using the Neubauer haemocytometer - Assessment of sperm morphology - Special treatment of biological fluids (semen)
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Ethnikon kai Kapodistriakon Panepistimion Athinon
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Greece
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Higher Education
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300 - 400 Employee
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Observer at Microbiology Laboratory
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Oct 2019 - Jan 2020
- Participation in various educational exercises of the Post-graduate Program "Molecular Medicine and Biopathology" conducted on the premises of the Microbiology Laboratory, School of Medicine, at Ethnikon and Kapodistriakon Panepistimion Athinon. - At the same time, I attended a significant part of the theoretical lectures of the Post-graduate Program "Pharmaceutical Microbiology", which were given at the Laboratory and on the premises of the company QACS (2 lectures on the quality assessment of pharmaceutical products, food and, cosmetics), familiarizing me with the practices in a clinical biochemistry and molecular diagnostics laboratory. Show less
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University of Patras
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Greece
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Higher Education
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700 & Above Employee
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Undergraduate Research Assistant
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Sep 2014 - Oct 2016
Experiments on the analysis of elongation translation factors through the Western blot method. An essential part of my Bachelor's thesis conducted at the Laboratory of Biological Chemistry under the supervision of both late Professor Dimitrios Kalpaxis and Professor Georgios Dinos of the Medical School, University of Patras The goal of the thesis was to study the effects of bioaccumulation of 3 heavy metals (copper, mercury and cadmium) on the modification of the expression and function of translation factors eEF2, (p)eEF2 and eEF1A1 at the elongation stage, via mechanisms of induced oxidative stress, in cells of the digestive gland of the Mediterranean mussel (Mytilus galloprovincialis). An additional goal was to quantify the oxidative damage, by measuring the carbonylation levels of total proteins, the FWR fraction, as well as the S-100 fraction, of the same mussels' cells after long-term exposure to heavy metals. Show less
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Education
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University of Patras
Bachelor of Science - BS, Biology/Biological Sciences, General