Diane (Dayeon) Kang
Graduate Student Researcher at California NanoSystems Institute at UCLA- Claim this Profile
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Bio
Experience
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California NanoSystems Institute at UCLA
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United States
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Higher Education
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1 - 100 Employee
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Graduate Student Researcher
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Jul 2023 - Present
∙ Currently developing standard operating procedures to automate bacterial plasmid purification (mini-preps) in the Living Biofoundry, a high-throughput automated platform for synthetic biology. ∙ Gained experience in using the ThermoFisher Laboratory Automation System (LAS) and Tecan Fluent automated liquid handler. ∙ Currently developing standard operating procedures to automate bacterial plasmid purification (mini-preps) in the Living Biofoundry, a high-throughput automated platform for synthetic biology. ∙ Gained experience in using the ThermoFisher Laboratory Automation System (LAS) and Tecan Fluent automated liquid handler.
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UCLA
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United States
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Higher Education
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700 & Above Employee
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Masters Student
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Apr 2023 - Present
∙ Designed and implemented a fully independent study of a novel Pseudomonas aeruginosa receptor-binding protein (“PAB92”), selected from an M13 phage display library, in preparation for master’s thesis. ∙ Successfully constructed a recombinant plasmid encoding PAB92 and performed heterologous protein overexpression in Escherichia coli and purification via IMAC.
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Teaching Assistant
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Apr 2023 - Jun 2023
∙ Taught laboratory sections 8+ hours per week for upper-division undergraduate CBE course 104D Molecular Biotechnology Lecture/Laboratory: From Gene to Product, which covers DNA cloning, cell culturing, fermentation, FPLC, and enzymatic assays for the expression and characterization of recombinant proteins.
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Undergraduate Research Assistant
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Jan 2021 - Mar 2023
∙ Screened for novel receptor-binding proteins targeting clinically relevant bacterial strains, such as Klebsiella pneumoniae, Pseudomonas aeruginosa, and Salmonella enterica, from an M13 phage display library of random peptides. ∙ Developed an assay to quantify M13 bacteriophage binding to target bacterial strains, exploring various experimental techniques such as flow cytometry, qPCR, and co-precipitation with magnetic beads. ∙ Analyzed the physicochemical, antibacterial, and toxicity properties of silver nanoparticle-M13 conjugates as a novel therapeutic for multi-drug resistant bacterial infections, gaining experience in TEM, DLS, bacterial growth curve assays, and cytotoxicity assays. Show less
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California NanoSystems Institute at UCLA
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United States
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Higher Education
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1 - 100 Employee
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Work-study Lab Assistant
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Nov 2021 - Mar 2023
∙ Prepared experiment kits for the CNSI Educational Outreach Program, which aims to encourage K-12 students to pursue studies in STEM fields by providing hands-on nanoscience experiments for local classrooms and on-campus summer programs. ∙ Leveraged academic and research background to revise and troubleshoot protocols for experiments covering a wide range of topics like biosensors, biotoxicity, and superhydrophobic surfaces. ∙ Prepared experiment kits for the CNSI Educational Outreach Program, which aims to encourage K-12 students to pursue studies in STEM fields by providing hands-on nanoscience experiments for local classrooms and on-campus summer programs. ∙ Leveraged academic and research background to revise and troubleshoot protocols for experiments covering a wide range of topics like biosensors, biotoxicity, and superhydrophobic surfaces.
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Sanofi
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France
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Pharmaceutical Manufacturing
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700 & Above Employee
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Medical Affairs Intern
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Mar 2021 - Sep 2021
∙ Presented on literature supporting Adacel® Tdap (tetanus, diphtheria, and pertussis) vaccination in pregnant women for passive neonatal immunity, contributing to regulatory affairs’ decision to push for Korean FDA approval of an expanded indication. ∙ Led scientific education on key evidence supporting the importance of influenza vaccination under the COVID-19 context to cross-functional teams, including marketing, regulatory affairs, and 40+ sales associates. ∙ Presented on literature supporting Adacel® Tdap (tetanus, diphtheria, and pertussis) vaccination in pregnant women for passive neonatal immunity, contributing to regulatory affairs’ decision to push for Korean FDA approval of an expanded indication. ∙ Led scientific education on key evidence supporting the importance of influenza vaccination under the COVID-19 context to cross-functional teams, including marketing, regulatory affairs, and 40+ sales associates.
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Education
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UCLA
Master of Science - MS, Biochemistry, Molecular and Structural Biology (BMSB) -
University of California, Los Angeles
Bachelor of Science - BS, Biochemistry